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[1]李会琳,路璐.多环芳烃对不同类型稻田土壤硝化潜势及氨氧化微生物的影响[J].应用与环境生物学报,2019,25(03):584-592.[doi:10.19675/j.cnki.1006-687x.201808011]
 LI Huilin,& LU Lu**.Effects of polycyclic aromatic hydrocarbons (PAHs) on nitrification potential and the abundance and community composition of ammonia-oxidizing* microorganisms in different types of paddy soils in China[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):584-592.[doi:10.19675/j.cnki.1006-687x.201808011]
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多环芳烃对不同类型稻田土壤硝化潜势及氨氧化微生物的影响
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
584-592
栏目:
研究论文
出版日期:
2019-06-25

文章信息/Info

Title:
Effects of polycyclic aromatic hydrocarbons (PAHs) on nitrification potential and the abundance and community composition of ammonia-oxidizing* microorganisms in different types of paddy soils in China
作者:
李会琳路璐
1西华师范大学环境科学与工程学院 南充 637009 2西华师范大学生命科学学院,西南野生动植物保护重点实验室 南充 637002
Author(s):
LI Huilin1 2 & LU Lu1**
1 College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China 2 Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong 637002, China
关键词:
农田土壤多环芳烃污染氨氧化细菌氨氧化古菌生态效应
Keywords:
agriculture soil PAHs contamination ammonia-oxidizing bacteria ammonia-oxidizing archaea ecological effect
分类号:
S154.1
DOI:
10.19675/j.cnki.1006-687x.201808011
摘要:
为揭示多环芳烃(PAHs)污染对我国不同区域稻田土壤氨氧化过程的影响,以8个省市的稻田土壤为研究对象,通过室内培养方式添加菲、荧蒽、苯并[a]蒽等3种PAHs进行28 d的培养实验,探究PAHs对不同土壤类型硝化潜势、氨氧化细菌(AOB)和古菌(AOA)丰度和群落结构的影响. 结果显示,8种土壤的理化性质、PAHs降解行为以及AOA和AOB菌群结构和丰度都有所差异,PAHs对硝化潜势的影响也表现为因土而异. PAHs显著抑制了黑龙江(黑土)、山西(褐土)、安徽(黄褐土)和湖南(黄壤)等4种土壤样品的硝化潜势(P < 0.05),显著促进了四川(石灰性紫色土)的硝化潜势,而对北京(潮土)、河南(潮土)和重庆(中性紫色土)土壤则没有显著影响. 荧光定量PCR分析amoA基因的丰度结果表明,在添加PAHs后,AOB的丰度显著增加,而AOA的丰度显著减少. 相关性分析表明土壤AOA的丰度与硝化潜势的变化规律有显著相关性(P < 0.05). 除重庆和四川土壤外,PAHs的添加降低了AOA和AOB群落Shannon多样性指数. 群落结构分析发现PAHs的添加促进了隶属于Nitrosospira cluster 3 的AOB相对丰度增加,而隶属于Nitosomonas cluster 7的AOB相对丰度则降低;然而PAHs对AOA群落结构则没有显著影响. 综上所述,PAHs污染可能通过改变土壤的氨氧化微生物的丰度和群落结构,进而在宏观上影响稻田土壤的硝化能力. (图7 表3 参46)
Abstract:
To reveal the effects of polycyclic aromatic hydrocarbons (PAHs) on ammonia oxidation processes in paddy soils, a laboratory incubation experiment was conducted to investigate the response of soil potential nitrification, ammonia-oxidizing archaea (AOA), and bacteria (AOB) to the addition of phenanthrene, fluoranthene, and benzo[a]pyrene in eight geographically different paddy soils in China. The results showed that the eight soils were different in physicochemical properties, PAHs degradation behaviors, and the structure and abundance of AOA and AOB. The impact of PAHs on soil nitrification potential activity also varied among different soils. The nitrification potential activities of soils in Heilongjiang (black soils), Shanxi (cinnamon soils), Anhui (yellow-cinnamon soils), and Hunan (yellow soils) soils were significantly inhibited by PAHs (P < 0.05), whereas the nitrification potential activity of Sichuan (calcareous purple soils) was stimulated. PAHs showed no significant effect on the nitrification potential activities of Beijing (fluvo-auric soils), Henan (fluvo-aquic soils) and Chongqing (neutral purple soils) soils. The abundance of AOA decreased significantly after the addition of PAHs, whereas the abundance of AOB increased. The correlation analysis showed there was a significant correlation between the abundance of AOA and nitrification potential activity in the soils (P < 0.05). The Shannon diversity index of AOA and AOB decreased remarkably in the presence of PAHs in the soils, except for Sichuan and Chongqing soil. AOB community structure was more affected by PAHs compared with that of AOA. The addition of PAHs stimulated the growth of AOB belonging to Nitrosospira cluster 3 and inhibited the growth of AOB belonging to Nitrosomonas cluster 7. PAHs had no significant effect on the AOA community structure. PAHs may alter the abundance and community structure of ammonia-oxidizing microorganisms in a directed manner, which could macroscopically affect the ecological functions of nitrification in paddy fields.

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更新日期/Last Update: 2019-06-25