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[1]宋永亭.不同配气条件下微生物驱油生长代谢规律[J].应用与环境生物学报,2017,23(06):974-978.[doi:10.3724/SP.J.1145.2016.12036]
 SONG Yongting**.Regulation of microbial growth and metabolism and oil displacement under different gas distribution conditions[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):974-978.[doi:10.3724/SP.J.1145.2016.12036]
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不同配气条件下微生物驱油生长代谢规律()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年06期
页码:
974-978
栏目:
微生物资源发掘与生物合成专栏论文
出版日期:
2017-12-25

文章信息/Info

Title:
Regulation of microbial growth and metabolism and oil displacement under different gas distribution conditions
作者:
宋永亭
中国石化胜利油田分公司石油工程技术研究院 东营 257000
Author(s):
SONG Yongting**
Research Institute of Petroleum Engineering, Shengli Oilfield Company, Sinopec, Dongying 257000, China
关键词:
配气氧化还原电位高通量测序功能菌物模驱油实验
Keywords:
gas distribution oxidation-reduction potential high-throughput sequencing functional bacteria physical simulation experiment
分类号:
Q939.97
DOI:
10.3724/SP.J.1145.2016.12036
摘要:
氧气是影响油藏内源微生物生长代谢及驱油效果的关键因素. 通过理论计算得出微生物生长代谢过程中对氧气的需要量,设计不同配气比实验,利用测定氧化还原电位的方法分析配气量对微生物生长代谢变化过程的影响,通过分子生物学高通量测序方法解析不同配气条件下微生物群落结构特征. 结果表明不同液气比条件下微生物群落结构组成存在差异,但随着培养时间的增加(30 d),氧气逐渐消耗,微生物群落趋向一致,微生物群落中主要存在6种驱油功能菌,其比例占到了所有种群的70%以上. 物模驱油实验得出液气比为1:5时不同种类的微生物能形成彼此相互稳定的微生物群落构成,驱替效率最高11.08%. 本研究明确了微生物驱油过程中适合的氧气配比,可为微生物驱油现场实施配气工艺参数的确定提供理论和数据支撑. (图8 表3 参26)
Abstract:
Oxygen is the key factor affecting growth and metabolism of microorganisms; hence, the influence of gas distribution on microbial growth and metabolism and oil displacement effect must be clarified before implementing microbial oil displacement in the field. In this study, the oxygen requirement for microbial growth and metabolism was determined, and a variety of gas distribution experiments were designed. Under different gas distribution conditions, the microbial growth state was determined using the oxidation-reduction potential, and microbial community structure characteristics were analyzed using high-throughput sequencing. Differences in the composition of the microbial community were found under different liquid–gas ratios. However, when the incubation time was increased (30 days), the composition of the microbial community under different liquid–gas ratios remained consistent because of oxygen consumption. Analysis of microbial community structure characteristics revealed six types of functional bacteria, and these accounted for > 70% of the community. The physical simulation experiment showed that the optimal displacement efficiency was 11.08%, when the liquid–gas ratio was 1:5. This study provides a reference for implementation of microbial oil displacement in the field.

参考文献/References:

1 Bao MT, Liu T, Chen Z. Guo LY, Jiang GC, Li YM, Li XM. A laboratory study for assessing microbial enhanced oil recovery [J]. Energy Sources, 2013, 35 (22): 2141-2148
2 曹嫣镔, 刘涛, 李彩风, 胡婧, 巴燕, 吴昕宇. 一株嗜热脂肪地芽孢杆菌的驱油性能及机理[J]. 应用与环境生物学报, 2015, 21(6): 1060-1064 [Cao YB, Liu T, Li CF, Hu J, Ba Y, Wu XY. Efficiency and mechanism of oil displacement by a strain of Geobacillus stearothermophilus [J]. Chin J Appl Environ Biol, 2015, 21 (6): 1060-1064]
3 王庭, 严群, 阮文权, 邹华. 生物法生成单质硫过程中氧化还原电位的研究[J]. 环境污染与防治, 2008, 30 (4): 44-47 [Wang T, Yan Q, Ruan WQ Zou H. Effect of ORP on biological oxidation of sulf ide species [J]. Environm Pollu Contr, 2008, 30 (4): 44-47]
4 黄志华, 张延平, 杜晨宇, 黄星, 曹竹安. 利用氧化还原电位调控基因工程菌株Klebsiella pneumoniae F-1合成1,3-丙二醇[J]. 过程工程学报, 2007, 7 (5): 1014-1017 [Huang ZH, Zhang YP, Du CY Huang X, Cao ZA. Improvement of 1,3-propanediol production with a recombinant strain klebsiella pneumoniae f-1 by regulating redox potential [J]. J Pro Eng, 2007, 7 (5): 1014-1017]
5 吉庆生, 高彦楼. 氧化还原电位法在复杂岩性油藏开发中的应用[J]. 大庆石油地质与开发, 2001, 20 (3): 71-73 [Ji QS, Gao YL. Application of oxidation reduction potential method in the development of complex lithologicreservoir [J]. Petrol GeolDev Daqing, 2001, 20 (3): 71-73]
6 Sunde E, Beeder J. Aerobic microbial enhanced oil recovery for offshore use [C]//SPE/DOE 24204. SPE/DOE Eighth Symposium on Enhanced Oil Recovery, Tulsa, Oklahoma, 1992
7 Zeng J, Zhao DY, Huang R Wu QL. Abundance and community composition of ammonia-oxidizing archaea and bacteria in two different zones [J]. Can J Microbiol, 2012, 58: 1018-1026
8 Schuster KC, Urlaub E, Gapes JR. Single-cell analysis of bacteria by Raman microscopy: spectral information on the chemical composition of cells and on the heterogeneity in a culture [J]. Microbiol Methods, 2000, 42 (1): 29-38
9 SY/T 6888-2012. 微生物驱油技术规范[S] [SY/T 6888-2012. Technical specification for microbial oil displacement [S]]
10 SY/T 5523-2000. 油气田水分析方法[S] [SY/T 5523-2000. Water analysis method for oil and gas fields [S]]
11 汪卫东, 魏斌, 谭云贤, 王修林. 微生物采油需要进一步解决的问题[J]. 石油勘探与开发, 2004, 31 (6): 88-91 [Wang WD, Wei B, Tan YX Wang XL. Problems confronted in microbial enhanced oil recovery [J]. Petrol Explor Dev, 2004, 31 (6): 88-91]
12 王慧, 宋智勇, 郝滨 刘涛, 高光军, 郭省学, 汪卫东. 微生物驱产出液群落结构与现场生产动态的关系[J]. 石油学报, 2013, 34 (3): 535-538 [Wang H, Song ZY, Hao BLiu T, Gao GJ, Guo SX, Wang WD. Study of the relationship between petroleum microbial community structure and oil production performance at microbial flooding oil field [J]. Petrol J, 2013, 34 (3): 535-538]
13 Hossein G, Farzaneh V, Alireza KS. Enhanced oil recovery from low permeability dolomite cores using biosurfactant produced by a Bacillus mojavensis (PTCC 1696) isolated from Masjed-I Soleyman field [J]. J Petrol Sci Eng, 2012, 81: 24-30
14 曾涛涛, 李冬, 谢水波 张杰. 厌氧氨氧化菌微生物特性研究进展[J]. 应用与环境生物学报, 2014, 20 (6): 1111-1116 [Zeng TT, Li D, Xie SB, Zhang J. A review on microbial properties of anaerobic ammonium oxidation (ANAMMOX) bacteria [J]. J Appl Environ Biol, 2014, 20 (6): 1111-1116]
15 宋智勇, 郭辽原, 袁书文, 郝斌, 吴晓玲. 高温油藏内源微生物的堵调及种群分布[J]. 石油学报, 2010, 31 (6): 975-979 [Song ZY, Guo LY, Yuan SW, Hao B, Wu XL. Microbial plugging and community distribution of indigenous thermophilic microbes in high-temperature oil reservoirs [J]. Acta Petrol Sin, 2010 , 31 (6): 975-979]
16 Tanwar P, Nandy T, Ukey P. Correlating on-line monitoring parameters, pH, DO and ORP with nutrient removal in an intermittent cyclic process bioreactor system [J]. Biores Technol, 2008, 99 (16): 7630-7635
17 Lo CK, Yu CW, Tam NFY. Enhanced nutrient removal by oxidation-reduction potential (ORP) controlled aeration in a laboratory scale extended aeration treatment system original [J]. Water Res, 1994, 28 (10): 2087-2094
18 Ellerby LM, Ellerby HM, Park SM. Shift of the cellular oxidation-reduction potential in neural cells expressing bcl-2 [J]. J Neurochem, 2002, 23: 1259-1267
19 Wang CH, Sinskey AJ. The role of oxidation-reduction potential in monitoring growth of cultured mammalian cells [J]. ProdBiol Anim Cells Cult, 1991, 15: 548-568
20 Felsberga J, Jelínkováa M, Kubizniakováb P. Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for identification of strictly anaerobic bacterium Zymophilus [J]. Anaerobe, 2015, 33: 85-89
21 ?atera M, Fanedla LJ, Malovrhb ?, Logar RM. Biogas production from brewery spent grain enhanced by bioaugmentation with hydrolytic anaerobic bacteria [J]. Biores Technol, 2015, 186: 261-269
22 Kumaki1 Y, Ogawa1 M, Hirano T. Family M42 aminopeptidase from the syntrophic bacterium Symbiobacterium thermophilum: characterization using recombinant protein [J]. J Biosci Bioeng, 2011, 111 (2): 134-139
23 Aizawa SI. Symbiobacterium thermophilum - a gram-negative, high (G+C) firmicutes [J]. Elec Microscop Images Bacter Flag Relat Surf Struc, 2014, 26: 84-85
24 乐建君, 刘芳, 张继元, 柏璐璐, 王蕊, 刘晓波, 侯兆伟, 伍晓林. 聚合物驱后油藏激活内源微生物驱油现场试验[J]. 石油学报, 2014, 35 (1): 99-106 [Le JJ, Liu F, Zhang JY Bo LL, Wang R, Liu XB, Hou ZW, Wu XL. A field test of activation indigenous microorganism for microbial enhanced oil recovery in reservoir after polymer flooding [J]. Petrol J, 2014, 35 (1): 99-106]
25 Nayak AS, Vijaykumar MH, Karegoudar TB. Characterization of biosurfactant produced by Pseudoxanthomonas sp. PNK-04 and its application in bioremediation [J]. Int Biodeterior Biodegrad, 2009, 63 (1): 73-79
26 Zhang F, Zhang Y, Chen Y. Simultaneous production of acetate and methane from glycerol by selective enrichment of hydrogenotrophic methanogens in extreme-thermophilic (70 ℃) mixed culture fermentation [J]. Appl Energy, 2015, 148 (15): 326-333

更新日期/Last Update: 2017-12-25