|本期目录/Table of Contents|

[1]宋永亭,胡婧,吴晓玲,等.室温条件下油藏采出液微生物群落结构稳定性[J].应用与环境生物学报,2017,23(03):495-501.[doi:2016.07026]
 SONG Yongting,HU Jing,WU Xiaoling,et al.Stability of microbial community structure in reservoir water samples at room temperature[J].Chinese Journal of Applied & Environmental Biology,2017,23(03):495-501.[doi:2016.07026]
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室温条件下油藏采出液微生物群落结构稳定性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年03期
页码:
495-501
栏目:
研究论文
出版日期:
2017-06-25

文章信息/Info

Title:
Stability of microbial community structure in reservoir water samples at room temperature
作者:
宋永亭胡婧吴晓玲曹嫣镔高光军孙刚正
中石化胜利油田石油工程技术研究院 东营 257000
Author(s):
SONG Yongting HU Jing WU Xiaoling CAO Yanbin GAO Guangjun & Sun Gangzheng
Research Institute of Petroleum Engineering and Technology, Shengli Oilfield Company, Sinopec, Dongying 257000, China
关键词:
高通量测序油藏微生物微生物多样性优势菌属群落结构稳定性
Keywords:
high-throughput sequencing reservoir microorganism microbial diversity predominant genus stability of community structure
分类号:
Q939.97 : TE357.9
DOI:
2016.07026
摘要:
获得准确的油藏微生物群落信息是开展油藏微生物工业化应用的重要前提. 为了解油藏采出液脱离油藏环境后微生物群落结构的变化以及一个时间点的单次取样的代表性,利用目前最先进的高通量测序技术,对胜利油田油藏采出液室温放置5 d内的微生物群落结构以及连续5 d独立取样的同一油井水样间的微生物群落结构开展研究. 结果显示,室内放置0-5 d过程中,细菌和古菌群落结构中的优势菌群没有发生明显改变,细菌的优势菌为热硫还原杆菌属(Thermodesulforhabdus)、无色杆菌属(Achromobacter)、沙雷氏菌(Serratia)以及一些未分类的细菌,古菌的优势菌为古生球菌属(Archaeoglobus)、产甲烷球菌属(Methanococcus)和甲烷鬃毛菌属(Methanosaeta). 但是细菌和古菌的多样性存在逐渐降低的趋势,其中古菌多样性的变化比细菌更为明显. 同时研究也证实同一口油井连续5 d独立取样的样品间,细菌和古菌群落结构中的优势菌群也没有发生较大的变化,与室内放置样品的优势菌属一致,多样性分析同样发现油藏内不同时间点古菌多样性的波动较细菌的大. 本研究说明油藏采出液室温放置短期内优势菌群结构具有稳定性,一次取样的油水井样品具有阶段代表性,这可为高通量测序技术在油藏微生物群落结构解析中的应用提供重要理论支撑. (图7 表1 参24)
Abstract:
The microbial community of reservoir water samples is the biological basis for industrial applications. In order to understand the stability of microbial community structure in reservoir samples after they are separated from the reservoir and the representativeness of single sampling, the microbial community structures of reservoir-produced fluid that was placed indoors for 5 days and continuous independent samples taken over 5 days were both analyzed by high-throughput sequencing technologies. The study confirmed that there was no obvious change in the dominant microbial community structure but that the diversity of both bacteria and archaea decreased with the extension of indoor placement time. Dominant bacteria in the fluid produced by Z3-X24 were Thermodesulforhabdus, Achromobacter, Serratia, and some unclassified bacteria. Dominant archaea were Archaeoglobus, Methanococcus, and Methanosaeta. The stability of bacterial diversity was higher than that of archaea in the course of indoor placement. The study also confirmed that the 5 days of independent samples also had a stable dominant microbial community structure. However, microbial diversity changed between samples from different sampling times. The study also found that the fluctuation of bacterial diversity in the samples was less than that of archaea. This study shows that the microbial community structure of reservoir fluid is stable in the short term, and that single sampling of reservoir fluid is representative. It provides important theoretical support for the application of high-throughput sequencing technology in the analysis of reservoir microbial community structure.

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更新日期/Last Update: 2017-06-25