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 FU Bo,WANG Weiwei,HAO Ying,et al.Isolation and Identification of Hydrogen-oxidizing Bacteria in Medicago sativa Rhizosphere[J].Chinese Journal of Applied & Environmental Biology,2009,15(05):650-654.[doi:10.3724/SP.J.1145.2009.00650]





Isolation and Identification of Hydrogen-oxidizing Bacteria in Medicago sativa Rhizosphere
付博王卫卫郝莹王莉娟唐明 陈兴都
(1西北大学生命科学学院,西部资源与现代生物技术教育部重点实验室 西安 710069)
(2西北农林科技大学林学部 杨凌 712100 )
(3西安建筑科技大学环境与市政工程学院 西安 710055)
FU Bo WANG Weiwei HAO Ying WANG Lijuan TANG Ming & CHEN Xingdu
(1Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’ an 710069, China)
(2College of Forest, Northwest A & F University, Yangling 712100, Shaanxi, China)
(3School of Environment and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China)
紫花苜蓿氢氧化细菌生理生化特性16S rDNA系统发育树假单胞菌属
Medicago sativa hydrogen-oxidizing bacteria physiological and biochemical characteristics 16S rDNA phylogenetic tree Pseudomonas
利用气体循环培养体系从陕西乾县HUP—豆科植物紫花苜蓿(Medicago sativa)根际土壤中分离获得37株细菌. 菌株氧化氢能力测定结果表明,8株菌氧化氢和自养生长能力较强,初步确定为氢氧化细菌类群;根据其形态特征、培养特征和生理生化特性,鉴定为7个不同属:假单胞菌属(Pseudomonas)、邻单胞菌属(Plesiomonas)、脂肪杆菌属(Pimelobacter)、黄色杆菌属(Xanthobacter)、勒米诺氏菌属(Leminorella)、地杆菌属(Terrabacter)和稀有杆菌属(Rarobacter);其中氧化氢能力最强的优势菌株WMQ-7 16S rDNA序列(GenBank登录号为EU807744)长度为1 451 bp,GC含量为53.8%,其核苷酸序列与假单胞菌属同源性高于99%,在系统发育树上位于同一分支,将WMQ-7菌株鉴定为假单胞菌属(Pseudomonas). 图2 表5 参16
Gas-cycle incubation system was used to enrich the Medicago sativa rhizosphere soil collected in Shaanxi Province, China and 37 strains of bacteria were isolated. Among them, 8 stains had stronger ability to oxidize hydrogen and could grow chemolithoautotrophically, so they were preliminarily identified as hydrogen-oxidizing bacteria. According to their cells, colony forms and biochemical characteristics, the 8 strains were identified as Pseudomonas, Plesiomonas, Pimelobacter, Xanthobacter, Leminorella, Terrabacter and Rarobacte. Strain WMQ-7 had the strongest ability to oxidize hydrogen, and its 16S rDNA sequence (GenBank accession number EU807744) was 1 451 bp and GC content was 53.8%. Strain WMQ-7 was clustered together with Pseudomonas in phylogenic tree, with the sequence identity of 99%. Therefore, strain WMQ-7 was identified as Pseudomonas. Fig 2, Tab 5, Ref 16


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国家自然科学基金重点项目(No. 30630054),科技部自然科技资源共享平台项目(No. DKA21207-7)和陕西省自然科学基金项目(No. SJ08-ZT03)资助 Supported by the Key Project of the National Natural Science Foundation of China ( No. 30630054), the Natural Resources Sharing Platform of the Ministry of Science and Technology of China (No. DKA21207-7), and the Natural Science Foundation of Shaanxi, China (No. SJ08-ZT03)
更新日期/Last Update: 2009-10-26