|本期目录/Table of Contents|

[1]邱吉国,郑金伟,张隽,等.乙羧氟草醚降解菌Pseudomonas sp. YF1的分离、鉴定与降解特性[J].应用与环境生物学报,2009,15(05):686-691.[doi:10.3724/SP.J.1145.2009.00686]
 QIU Jiguo,ZHENG Jinwei,ZHANG Jun,et al.Isolation, Identification and Characteristics of a Fluoroglycofen-ethyl-degrading Bacterium YF1[J].Chinese Journal of Applied & Environmental Biology,2009,15(05):686-691.[doi:10.3724/SP.J.1145.2009.00686]
点击复制

乙羧氟草醚降解菌Pseudomonas sp. YF1的分离、鉴定与降解特性()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年05期
页码:
686-691
栏目:
研究论文
出版日期:
2009-10-25

文章信息/Info

Title:
Isolation, Identification and Characteristics of a Fluoroglycofen-ethyl-degrading Bacterium YF1
作者:
邱吉国郑金伟张隽李顺鹏何健
(南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室 南京 210095)
Author(s):
QIU Jiguo ZHENG Jinwei ZHANG Jun LI Shunpeng & HE Jian
(Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
乙羧氟草醚假单胞菌生物降解降解特性
Keywords:
Fluoroglycofen-ethyl Pseudomonas biodegradation degradation characteristics
分类号:
X172
DOI:
10.3724/SP.J.1145.2009.00686
文献标志码:
A
摘要:
从生产乙羧氟草醚工厂的污水处理池污泥中分离到一株乙羧氟草醚降解细菌,命名为YF1. 根据表型特征、生理生化特性和16S rDNA序列系统发育分析,将其鉴定为假单胞菌属(Pseudomonas sp.). 接种量为5%时,菌株YF1在含200 mg/L乙羧氟草醚的基础盐液体培养基中降解乙羧氟草醚,7 d后降解率约80%. 加大接种量和外加营养碳氮源可以促进乙羧氟草醚的降解. 该菌株降解乙羧氟草醚的最适pH为7.0,最适温度为30 ℃. 菌株YF1能利用苯酚、邻苯二酚、对苯二酚、苯甲酸、龙胆酸、对硝基苯酚和邻氯苯酚为底物生长,不能利用3-苯氧基苯甲酸为碳源生长,菌株YF1细胞内邻苯二酚1,2-双加氧酶受到乙羧氟草醚或其代谢产物的诱导. 图5 表1 参25
Abstract:
The use of organofluorine compounds has been increased in recent years, and some of the compouds are now ubiquitous environmental contaminants. Although generally viewed as recalcitrant because of their lack of chemical reactivity, a lot of fluorinated organisms are biologically active. The pesticides containing fluorine is now the hot spot of research and development. Fluoroglycofen-ethyl is one kind of herbicides having been widely used recently. A bacterial strain designated as strain YF1, capable of degrading fluoroglycofen-ethyl efficiently, was isolated from sludge in a wastewater treatment installation that had long been used to treat fluoroglycofen-ethyl polluted water. Strain YF1 was identified preliminarily as Pseudomonas sp. based on its physiological and biochemical characters and the result of the 16S rDNA homologue sequence analysis. This bacterium could degrade 80% of 200 mg/L fluoroglycofen-ethyl in 7 d. Large initial inoculum size and additional nutrients could promote the degradation. The optimal pH and temperature for fluoroglycofen-ethyl degradation were 7.0 and 30 ℃, respectively. Strain YF1 used phenol, catechol, hydroquinol, benzoic acid, gentisic acid, p-nitrophenol and o-chlorophenol, but not 3-phenoxybenzoate as sole carbon sources. Catechol 1,2-dioxygenase, but not catechol 2,3-dioxygenase and gentisic acid 1,2-dioxygenase, was induced by fluoroglycofen-ethyl in strain YF1 cells. Fig 5, Tab 1, Ref 25

参考文献/References:

1 Zhang YB (张一宾), Qian YY (钱跃言). Structure speciality and researching development survey of pesticide containing fluorine. Jiangsu Chem Ind (江苏化工), 2002, 30 (2): 13~15
2 Zhang GZ (张广忠), Yang YR (杨燕茹), Hao YA (郝爱友). 含氟农药发展概况. Organo-Fluorine Ind (有机氟工业), 2004, 2: 28~29
3 Key BD, Howell RD, Criddle CS. Fluorinated organics in the biosphere. Environ Sci Technol, 1997, 31 (9): 2445~2454
4 Yu H (余慧), Zhou Q (周琪). Biological transformation of organofluorine compounds. Sichuan Environ (四川环境), 2005, 24 (4): 54~58
5 Ling Y (凌云), Chu XG (储晓刚), Yong W (雍炜), Wang DN (王大宁), Cui XY (崔新仪). Degradation of fipronil during making pickle. Chin J Food Hygiene (中国食品卫生杂志), 2007, 19 (6): 495~498
6 Guo M (郭敏), Shan ZJ (单正军), Shi LL (石利利). Studies on the degradation characteristics of Fipronil in three kinds of soils. Chin J Pesticide Sci (农药学学报), 2008, 10 (3): 335~342
7 Zong HM (宗虎民), Ma DY (马德毅), Wang JY (王菊英), Hu JT (户江涛). Effects of florfenicol on microbial activity in marine sediment. Chin J Appl Environ Biol (应用与环境生物学报), 2008, 14 (3): 408~412
8 Yu HF (于海峰). 乙羧氟草醚的生物学特性研究. Pesticide (农药), 2001, 40 (5): 31~36
9 Liu CL (刘长令). 世界农药大全除草剂卷. Beijing, China (北京): Chemical Industry Press (化学工业出版社), 2002. 190~192
10 Wu LY (吴凌云), Tao CJ (陶传江), Pu XY (朴秀英), Jiang H (姜辉), Li XF (李学锋), Wang CJ (王成菊). Residue analysis and degradation dynamics of fluoroglycofen-ethyl in soils. J Agro-Environ Sci (农业环境科学学报), 2006, 25 (6): 1659~1662
11 Wu LY (吴凌云), Tao CJ (陶传江), Zong FL (宗伏霖), Pu XY (朴秀英), Jiang H (姜辉), Li XF (李学锋), Wang CJ (王成菊). Degradation dynamics of fluoroglycofen-ethyl in water. Chin J Pesticide Sci (农药学学报), 2007, 9 (1): 96~99
12 Laganà A, Fago G, Fasciani L, Marino A, Mosso M. Determination of diphenyl-ether herbicides and metabolites in natural waters using high-performance liquid chromatography with diode array tandem mass spectrometric detection. Anal Chim Acta, 2000, 414 : 79~94
13 Cheng GF (程国锋), Li SP (李顺鹏), Shen B (沈标), Yang JH (杨军辉). Microbial degradation of residual pesticides in vegetable. Chin J Appl Environ Biol (应用与环境生物学报), 1998, 4 (1): 81~84
14 Topp E, Zhu H, Nour S, Houot S, Lewis M, Cuppels D. Characterization of an atrazine-degrading Pseudaminobacter sp. isolated from Canadian and French agricultural soils. Appl Envir Microbiol, 2000, 66 (7): 2773~2782
15 Zheng JL (郑金来), Li JW (李君文), Chao FH (晁福寰). Advance and prospect of microorganism degrading common pesticide. Res Environ Sci (环境科学研究), 2001, 14 (2): 62~64
16 Yang XH (杨小红), Li J (李俊), Ge C (葛诚), Shen DL (沈德龙). Nonel advanceson pesticides degradation by microognisms. Microbiology (微生物学通报), 2003, 30 (6): 93~96
17 Gou M (苟敏), Qu YY (曲媛媛), Yang H (杨桦), Zhou JT (周集体), Li A (李昂), Guan XY (关晓燕), Ai FF (艾芳芳). Sphingomonas sp.: A novel microbial resource for biodegradation of aromatic compounds. Chin J Appl Environ Biol (应用与环境生物学报), 2008, 14 (2): 276~282
18 Dong XZ (东秀珠), Cai MY (蔡妙英). Identification Manual of Common Bacteria System. Beijing, China (北京): Science Press (科学出版社), 2001. 370~410
19 奥斯伯F, 布伦特R, 金斯顿RE. Short Protocols in Molecular Biology. Beijing, China (北京): Science Press (科学出版社), 1999. 39
20 Shen XH (沈锡辉), Liu ZP (刘志培), Wang BJ (王保军), Liu SJ (刘双江). Isolation, identification of phenol-degrading Rhodococcus sp. strain PNAN5 and characterization of its ring-cleavage dioxygenases. Acta Sci Circumst (环境科学学报), 2004, 24 (3): 482~486
21 Ren HS (任河山), Wang Y (王颖), Zhao HB (赵化冰), Cai BL (蔡宝立). Isolation and identification of phenol-degrading strains and the application in biotreatment of phenol-containing wastewater. Environ Sci (环境科学), 2008, 29 (2): 482~487
22 Tallur PN, Megadi VB, Ninnekar HZ. Biodegradation of cypermethrin by Micrococcus sp. strain CPN1. Biodegradation, 2008, 19 (1): 77~82
23 Zhou NY, Fuenmayor SL, Williams PA. nag genes of Ralstonia (formerly Pseudomonas) sp. strain U2 encoding nzymes for gentisate catabolism. J Bacteriol, 2001, 183: 700~708
24 Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem, 1976, 72 (1): 248~254
25 Yang SS (杨苏声). 细菌分类学. Beijing, China (北京): China Agriculture Press (中国农业出版社), 1997. 1~7

相似文献/References:

[1]叶婷 姚陈 李茜茜 张红星 李 林.假单胞菌表达载体pYMB03的构建与性能分析[J].应用与环境生物学报,2008,14(04):528.
[2]陈谦,张新雄,赵海,等.生物有机肥中几种功能微生物的研究及应用概况[J].应用与环境生物学报,2010,16(02):294.[doi:10.3724/SP.J.1145.2010.00294]
 CHEN Qian,ZHANG Xinxiong,ZHAO Hai,et al.Advance in Research and Application of Some Functional Microbes in Bio-organic Fertilizer[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):294.[doi:10.3724/SP.J.1145.2010.00294]
[3]张亮,何晓红,张礼霞,等.一株假单胞菌MBR对亚碲酸盐的好氧还原特征[J].应用与环境生物学报,2011,17(01):126.[doi:10.3724/SP.J.1145.2011.00126]
 ZHANG Liang,HE Xiaohong,ZHANG Lixia,et al.Characterization of Aerobic Reduction of Tellurite by Pseudomonas sp. MBR[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):126.[doi:10.3724/SP.J.1145.2011.00126]
[4]杨薇薇,张亮,占国强,等.假单胞菌MBR还原钛(IV)合成钛纳米粒子[J].应用与环境生物学报,2012,18(02):276.[doi:10.3724/SP.J.1145.2012.00276]
 YANG Weiwei,ZHANG Liang,ZHAN Guoqiang,et al.Reduction of Ti(IV) and Synthesis of Titanium Nanoparticles by Pseudomonas sp. MBR[J].Chinese Journal of Applied & Environmental Biology,2012,18(05):276.[doi:10.3724/SP.J.1145.2012.00276]
[5]龙建友,罗定贵,陈永亨.一株Tl+抗性菌株的鉴定及吸附特性[J].应用与环境生物学报,2014,20(03):426.[doi:10.3724/SP.J.1145.2014.10004]
 LONG Jianyou,LUO Dinggui,CHEN Yongheng.Identification and biosorption characterization of a thallium-resistant strain[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):426.[doi:10.3724/SP.J.1145.2014.10004]
[6]徐梦宇,刘艳杰,林晖,等.假单胞菌催化的碳碳双键不对称还原[J].应用与环境生物学报,2014,20(05):798.[doi:10.3724/SP.J.1145.2014.03034]
 XU Mengyu,LIU Yanjie,LIN Hui,et al. Asymmetric reduction of C=C bond catalyzed with Pseudomonas species[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):798.[doi:10.3724/SP.J.1145.2014.03034]

备注/Memo

备注/Memo:
国家高技术研究发展计划(863计划,No. 2006AA10Z402)和国家自然科技平台项目(No. 2005DKA21201-2) Supported by the National High-tech Research and Development Program of China (863 Program, No. 2006AA10Z402) and the National Technology Platform Program of China (No. 2005DKA21201-2)
更新日期/Last Update: 2009-10-26