|本期目录/Table of Contents|

[1]李毳,柴宝峰.基于分子标记的油松种群遗传保护分析[J].应用与环境生物学报,2009,15(04):459-463.[doi:10.3724/SP.J.1145.2009.00459]
 LI Cui,CHAI Baofeng.Genetic Conservation of Pinus tabulaeformis Population Implicated by Molecular Markers[J].Chinese Journal of Applied & Environmental Biology,2009,15(04):459-463.[doi:10.3724/SP.J.1145.2009.00459]
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基于分子标记的油松种群遗传保护分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年04期
页码:
459-463
栏目:
研究论文
出版日期:
2009-08-25

文章信息/Info

Title:
Genetic Conservation of Pinus tabulaeformis Population Implicated by Molecular Markers
作者:
李毳柴宝峰
1山西财经大学环境经济学院,2山西大学生物技术研究所 太原 030006
Author(s):
LI CuiCHAI Baofeng
1School of Environment Economics, Shanxi University of Finance and Economics, Taiyuan 030006, China
2Institute of Biotechnology, Shanxi University, Taiyuan 030006, China
关键词:
油松分子标记遗传多样性种群结构遗传保护
Keywords:
Pinus tabulaeformismolecular markergenetic diversitypopulation structuregenetic conservation
分类号:
S791.254.01
DOI:
10.3724/SP.J.1145.2009.00459
文献标志码:
A
摘要:
利用分子标记技术分析了山西5个天然油松种群140个个体的遗传特征,比较了不同种群的多态位点显性频率、遗传多样性指数、基因流和分化系数. 筛选的5个ISSR引物和5个RAPD引物共扩增到位点68个,其中多态位点50. 遗传参数的统计分析显示,多态位点的显性频率、遗传多样性指数、分化系数分别介于0.000 0~1.000 0、0.020 0~0.500 0和0.003 7~0.615 6之间,表明油松种群遗传多样性的主要来源是等位基因频率的不同和部分位点较大程度的遗传分化. 根据油松的种群遗传学特征,本研究认为只有加强天然油松林的生境保护,使其不产生片断化,才能使油松的遗传资源得到有效保护. 图2 表4 参16
Abstract:
Pinus tabulaeformis widely distributions in China from northeast to southwest, and plays an important role in natural forests and artificial plantations. Its genetic characteristics of DNA in 140 samples from 5 populations in Shanxi were investigated by using molecular technique. A total of 68 reproducible bands were identified using 5 RAPD primers and 5 ISSR primers, in which 50 bands were polymorphicl loci. The statistic analysis of the genetic parameters indicated that gene frequency of dominants, genetic diversity index and the relative magnitude of differentiation among populations were 0.000 0~1.000 0, 0.020 0~0.500 0 and 0.003 7~0.615 6, respectively, suggesting that the genetic diversity of P. tabulaeformis populations in Shanxi mainly lies on different allelic frequencies and on some high level genetic differentiating locus. Based on the characteristics of P. tabulaeformis populations in Shanxi, we suggested that only by protecting it’s territory and keeping them integration, could the genetic resource of pine be well conserved. Fig 2, Tab 4, Ref 16

参考文献/References:

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备注/Memo

备注/Memo:
山西省回国留学人员科研基金(No. 20 0846)和国家自然科学基金(No. 30770294)资助
更新日期/Last Update: 2009-08-26