|本期目录/Table of Contents|

[1]陈文峰,李洪波.3株紫花苜蓿根瘤菌sinR基因的克隆、序列测定及系统发育分析[J].应用与环境生物学报,2008,14(02):240-243.
 CHEN Wenfeng,et al..Cloning, Sequencing and Phylogenetic Analysis of sinR Gene in Three Rhizobia Associated with Alfalfa[J].Chinese Journal of Applied & Environmental Biology,2008,14(02):240-243.
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3株紫花苜蓿根瘤菌sinR基因的克隆、序列测定及系统发育分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
14卷
期数:
2008年02期
页码:
240-243
栏目:
综述
出版日期:
2008-02-15

文章信息/Info

Title:
Cloning, Sequencing and Phylogenetic Analysis of sinR Gene in Three Rhizobia Associated with Alfalfa
作者:
陈文峰李洪波
Author(s):
CHEN Wenfenget al.
(Key Laboratory of AgroMicrobial Resource and Application, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100094, China)
关键词:
关键词 群体感应根瘤菌 sinR基因紫花苜蓿草木樨中华根瘤菌
Keywords:
Keywords quorum sensing rhizobia sinR gene Medicago sativa Sinorhizobium meliloti
Abstract:
Abstract In order to clone sinR gene from the three different strains of rhizobia (CCBAU 30085, USDA 1002 and CCBAU QH180) associated with alfalfa, one pair of primers to sinR gene was designed and synthesized according to the literature[1]. The sinR genes of the three strains were amplified by PCR and the PCR products were ligated into pGEM-T-easy vector, which was then transformed into Escherichia coli DH5α. Positive bacterial clones were screened by bluewhite colony and Amp resistance. The sinR genes of the three strains were sequenced and compared with the known sinR gene (GenBank No. AL591788) homologously and phylogenetically. The sinR genes of these three strains were cloned successfully. The results from the homologous and phylogenetic analyses showed that the sinR genes of the three rhizobial strains had very high similarity of 99% and had a similarity of 99.7%~100% to the known sinR gene in the strain of Sinorhizobium meliloti 1021. However, they had only 45% similarity to the raiR genes in the Rhizobium leguminosarum and R. etli. This may suggest that though long evolution of rhizobia associated with alfalfa, the sinR genes in these strains were still considerably conservative. Fig 2, Ref 17

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更新日期/Last Update: 2008-04-23