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[1]韩雪花 胡瀚文 罗 禹** 邓 赟**.白及内生菌Penicillium oxalicum化学成分*[J].应用与环境生物学报,2019,25(02):1-9.[doi:10.19675/j.cnki.1006-687x.2018.07020]
 HAN XueHua,HU HanWen,LUO Yu**& DENG Yun**.CLC R914hemical constituents of Penicillium oxalicum, an endophytic fungus isolated from Bletilla striata (Thunb.) Reichb. f.[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):1-9.[doi:10.19675/j.cnki.1006-687x.2018.07020]
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白及内生菌Penicillium oxalicum化学成分*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
1-9
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
CLC R914hemical constituents of Penicillium oxalicum, an endophytic fungus isolated from Bletilla striata (Thunb.) Reichb. f.
文章编号:
201807020
作者:
韩雪花1 胡瀚文1 罗 禹2** 邓 赟1**
1 成都中医药大学药学院 中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室省部共建国家重点实验室培育基地 成都 611137
2 西南医科大学药学院 泸州 646000
Author(s):
HAN XueHua1 HU HanWen1 LUO Yu2**& DENG Yun1**
1Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, State Key Laboratory Breeding of Systematic Research Development and Utilization of Chinese Medical Resources, Co-founded by Sichuan Province and MOST, Pharmacy Department, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China?
2School of Pharmacy, Southwest Medical University, Luzhou 646000, China
关键词:
白及草酸青霉菌化学成分分离鉴定
Keywords:
Penicillium oxalicum Bletilla striata (Thunb.) Reichb. f. chemical constituents isolation and elucidation
DOI:
10.19675/j.cnki.1006-687x.2018.07020
摘要:
从中药白及新鲜药材中分离得到一株内生菌草酸青霉菌Penicillium oxalicum进行固体发酵40发酵产物采用75%乙醇渗漉提取后用乙酸乙酯萃取,并利用硅胶柱色谱、Sephadex LH-20凝胶、HPLC等方法从中分离得到15个单体化合物,化合物经质谱、核磁共振等波谱方法鉴定为:α-亚麻酸甲酯(1)、亚油酸甲酯(2)、benzoic acid(3)、4-hydroxybenzaldehyde(4)、ganodermasides D(5)、dankasterone A(6)、calvasterol B(7)、cyclo-(Pro-Phe)(8)、penioxalicin(9)、5α-麦角甾-7,22-二烯-3β,5,6β-三醇(10)、cis-4-hydroxyscytalone(11)、3,4-dihydroxyphenylacetic acid methyl ester (12)、protocatechuic acid(13)、cyclo-(4-S-hydroxy-R-proline-R-isoleucine)(14)、cyclo-L-(4-hydroxyprolinyl)-L-leucine(15)。其中化合物14-15均为首次从P. oxalicum中分离得到。
Abstract:
To study the isolation and elucidation of chemical constituents of Penicillium oxalicum, an endophytic fungus isolated from Bletilla striata (Thunb.) Reichb. f.. After solid state fermentating for 40 days, secondary metabolites were percolated by ethanol-water(75:25 V/V), extracted with ethyol acetate and purified by column chromatography methods such as silica gel, Sephadex LH-20 as well as HPLC, 15 compounds were isolated. Their structures were elucidated through extensive spectroscopic analyses as follows: α-methyl linolenic acid (1), methyl linoleate (2), benzoic acid (3), 4-hydroxybenzaldehyde (4), ganodermasides D (5), dankasterone A (6), calvasterol B (7), cyclo-(Pro-Phe) (8), penioxalicin (9), 5α-ergoside-7,22-diene-3β,5,6β-triol (10), cis-4-hydroxyscytalone (11), 3,4-dihydroxyphenylacetic acid methyl ester (12), protocatechuic acid (13), cyclo-(4-S-hydroxy-R-proline-R-isoleucine) (14), cyclo-L-(4-hydroxyprolinyl)-L-leucine (15). Among them, compounds 1 and 4-15 were isolated from P. oxalicum for the first time. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ??

备注/Memo

备注/Memo:
收稿日期: 2018-07-13 接受日期 Accepted: 2018-08-10*国家自然科学基金面上项目(81373961)、国家自然基金国家基础科学人才培养项目(J13100340)和四川省科技厅省青年科技创新研究团队专项计划(2014TD0007)资助 **通讯作者(E-mail: dengyun2000@hotmail.com; uoyu@163.com)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-08-30