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[1]马贵党 李崇华 王 飞 董彬彬 沈 越 沈 燕 葛 滢**.一株铜抗性细菌的分离鉴定及其耐铜机制*[J].应用与环境生物学报,2019,25(02):1-11.[doi:10.19675/j.cnki.1006-687x.2018.06025]
 MA Gui-dang,LI Chong-hua,WANG Fei,et al.Isolation and Identification of a Cu R esistant Bacterial Strain and Its Cu Resistance Mechanism*[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):1-11.[doi:10.19675/j.cnki.1006-687x.2018.06025]
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一株铜抗性细菌的分离鉴定及其耐铜机制*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
1-11
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Isolation and Identification of a Cu R esistant Bacterial Strain and Its Cu Resistance Mechanism*
文章编号:
201806025
作者:
马贵党1 李崇华1 王 飞2 董彬彬1 沈 越1 沈 燕1 葛 滢1**
1南京农业大学资源与环境科学学院 江苏省海洋生物学重点实验室 南京 210095?
2中钢集团马鞍山矿山研究院有限公司 马鞍山 243000
Author(s):
MA Gui-dang1 LI Chong-hua1 WANG Fei2 DONG Bin-bin1 SHEN Yue1 SHEN Yan1 & GE Ying1**
1College of Resource of Environmental Science, Nanjing agricultual university , Nanjing 210095, China?
2Sinosteel Maanshan Mining Research Institute Co.Ltd, Maanshan 243000, China
关键词:
不动杆菌生物修复耐性机制 胞外聚合物
Keywords:
Acinetobacter sp. copper bioremediation mechanisms of resistance EPS
DOI:
10.19675/j.cnki.1006-687x.2018.06025
摘要:
利用微生物修复铜污染环境是当前研究的热点之一,筛选铜污染环境生物修复的菌种,研究其耐铜机制对铜污染土壤修复具有重要的意义。本文从铜矿附近的土壤中分离得到一株具有较强铜抗性的细菌,经形态观察、生理生化实验及系统发育分析,鉴定该菌株为不动杆菌属,命名为Acinetobacter sp. MA9。为探索MA9对铜的耐性、富集及其可能机制,研究了不同浓度铜处理下菌株的生长和细菌对培养基铜的去除效率,同时分析了铜胁迫下MA9胞外聚合物的含量、菌体表面形貌和官能团的变化。结果表明,铜处理细菌36 h后,以培养基铜浓度表征的半数效应浓度(EC50)值为251 mg/L,细菌对溶液铜的去除量最高达到了溶液铜浓度的68%。与对照相比,铜胁迫使得MA9产生了更多的胞外聚合物,其中多糖增加62%,蛋白增加185%。利用扫描电镜观察发现,与无铜处理的菌株相比,铜处理菌体表面存在大量颗粒物,能谱分析也显示,MA9细胞表面检测到铜,这说明铜可能与细菌代谢物结合产生胞外吸附。傅里叶红外光谱分析结果表明,菌株在铜处理后细胞表面与醛基官能团相关的吸收峰消失,说明铜主要和菌体表面的醛基官能团结合。综上所述,胞外吸附和胞外沉淀是不动杆菌MA9主要的耐铜机制,醛基是参与MA9和铜反应的主要基团,这一研究结论为MA9在铜污染环境修复中的应用奠定了基础。图6表2参36
Abstract:
Microbial remediation of copper (Cu) pollution is one of the key topics and received massive attentions. Screening the bacterial strains that are able to remediate Cu contaminated environment, and uncoupling the underlying Cu resistance mechanisms are of great significance for engineering practices to clean up Cu polluted soils. A bacterial strain with strong Cu resistance was isolated from a soil close to a Cu mine. This bacterium was identified and named as Acinetobacter sp. MA9, based on the morphological, physiological and biochemical experiments and 16S rRNA gene sequence analysis. In order to explore the Cu tolerance, enrichment and their possible mechanism, the growth of MA9 and its Cu removal efficiency from the culture medium were studied under different Cu treatments. Variations of the extracellular polymeric substances (EPS), surface morphology and functional groups of MA9 under Cu stress were also analyzed. The results showed that the concentration for 50% of maximal effect (EC50) value of the MA9 treated with Cu for 36 h was 251mg/L, and the highest Cu removal rate was 68% of the Cu concentration in the solution. Compared with the control, Cu stress resulted in more EPS production by MA9, in which polysaccharide increased by 62% and protein increased by 185%. Scanning electronic microscope (SEM) showed that a large number of particles formed on the surface of the bacterial cells treated with Cu. Energy Dispersive Spectrometry (EDS) analysis also showed that Cu was detected on the surface of MA9 cells, suggesting that Cu might be bound extracellularly with bacterial metabolites. Fourier Transform Infrared Spectroscopy (FTIR) demonstrated that the absorption peaks related to aldehyde functional groups disappeared after Cu treatment, indicating that these groups on the cell surface were involved in the Cu binding. In conclusion, extracellular adsorption and precipitation may be the main Cu resistance mechanism of Acinetobacter sp. MA9. Aldehyde group may be the major group involved in the reaction of MA9 and Cu. The results provide a foundation for the application of MA9 in the remediation of Cu polluted environment.

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备注/Memo

备注/Memo:
收稿日期: 2018-06-18 接受日期 Accepted: 2018-07-04
*国家重点研发计划(2017YFD0800305)、南京农业大学--中钢集团马鞍山矿山研究院有限公司合作项目(030-HM0027)和江苏省大学生创业训练项目(201710307005T)资助?
**通讯作者(E-mail: yingge711@njau.edu.cn)
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更新日期/Last Update: 2018-08-02