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[1]张毓森,叶军 苏建强**.粪肥与铜停止施用后对农田土壤抗生素抗性基因的长期影响*[J].应用与环境生物学报,2019,25(02):1-9.[doi:10.19675/j.cnki.1006-687x.2018.06020]
 ZHANG Yusen,YE Jun & SU Jianqiang **.Long-term effect of one-time application of manure or copper on antibiotic resistance genes in agricultural soil *[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):1-9.[doi:10.19675/j.cnki.1006-687x.2018.06020]
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粪肥与铜停止施用后对农田土壤抗生素抗性基因的长期影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
1-9
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Long-term effect of one-time application of manure or copper on antibiotic resistance genes in agricultural soil *
文章编号:
201806020
作者:
张毓森1 2 叶军1 苏建强1**
1中国科学院城市环境研究所 厦门 361021
2中国科学院大学 北京 100049
Author(s):
ZHANG Yusen1 2 YE Jun1 & SU Jianqiang 1**
1Institute of Urban Environment, Chinese academy of sciences, Xiamen 361021, China?
2University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
抗生素抗性基因粪肥高通量定量聚合酶链式反应
Keywords:
Antibiotic resistance genes manure copper high-throughput quantitative polymerase chain reaction
DOI:
10.19675/j.cnki.1006-687x.2018.06020
摘要:
人类农业生产活动显著影响农田生态系统中抗生素抗性基因的增殖与扩散,很多研究表明重金属和粪肥施用会导致农田生态系统抗生素抗性基因的增殖,关于停止施用猪粪和重金属后对土壤抗性基因长期影响的研究较为匮乏。为探究粪肥与铜施加对土壤抗生素抗性基因增殖与传播的长期影响,采用高通量荧光定量聚合酶链式反应分析了猪粪和铜停施10年后土壤中抗生素抗性基因多样性和丰度。结果显示:猪粪和铜停施十年后,土壤抗生素抗性基因相对丰度仍分别处于2.16倍和2.01倍于对照组的较高水平,说明猪粪和铜施用可导致土壤抗生素抗性基因的长期存在,其中ycel_mdtH和cphA基因在猪粪和铜施用两种处理中均显著富集。PCA结果显示,猪粪和铜处理土壤中抗生素抗性基因的分布格局无明显差别,铜处理土壤中所检出的抗生素抗性基因数量的90%以上均可在猪粪施用土壤中检出,而铜是唯一在两种处理土壤中检出浓度均显著高于(P<0.001)对照并处于同一浓度水平的元素。研究表明铜可能是导致土壤中抗生素抗性基因增殖扩散的主要因素之一,揭示了施加重金属含量较高的粪肥对农田土壤抗生素抗性基因有着长期影响的风险,从而对农业中的粪肥施用提供了新的风险评价参数。(图4 参24)
Abstract:
The proliferation and spread of antibiotic resistance genes (ARGs) in agricultural ecosystem have been significantly impacted by human agricultural activities, including heavy metal and animal manure application. However, previous studies mainly focus on the impact of periodically application of manures on ARGs in soil, the persistence of ARGs after one-time application of manure and heavy metals remain poorly understood. In this study, high throughput quantitative polymerase chain reaction was performed to investigate the diversity and relative abundance of ARGs in long-term experimental soils, which had been treated by manure or copper 10 years ago. The results showed higher relative abundance of ARGs were sustainable after stopping treating for 10 years, in which the relative abundance of ARGs were 2.16 and 2.01 times of control soil, indicating the persistence and enrichment of ARGs in manure or copper treated soil, especially ycel_mdtH and cphA gene. Principal component analysis showed similar ARG profiles between manure treatments and copper treatments, with 90% of detected ARGs were shared between copper treatments and manure treatment. Additionally, copper was the unique element that were frequently detected from both manure and copper treatments, suggesting that that copper might be one of the major factors that influence on the enrichment of ARGs in soil. The results revealed the long-term risk of applying metal enriched manure into agricultural soil, so that provided a new parameters to assessment of manure applications in agriculture.

相似文献/References:

[1]王柳红 奚 慧 黄兴华 孙金昭 谢 冰**.城市垃圾填埋场抗生素抗性基因的污染特征*[J].应用与环境生物学报,2019,25(02):1.[doi:10.19675/j.cnki.1006-687x.2018.06039]
 WANG Liuhong,X I Hui,H UANG Xinghua,et al.Characteristics of Antibiotic Resistance Genes in Municipal Solid Waste Landfill*[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):1.[doi:10.19675/j.cnki.1006-687x.2018.06039]

备注/Memo

备注/Memo:
收稿日期: 2018-06-11 接受日期 Accepted: 2018-08-06
*国家重点研发计划重点专项项目(2016YFD0800205)资助
**通讯作者(E-mail: jqsu@iue.ac.cn)
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更新日期/Last Update: 2018-08-30