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[1]晋 蕾# 周应敏# 李才武 吴代福 李 果 何永果 李 蓓 黄 炎 张和民** 邹立扣**.野化培训与放归、野生大熊猫肠道菌群的组成和变化*[J].应用与环境生物学报,2019,25(01):1-10.[doi:10.19675/j.cnki.1006-687x.2018.06007]
 JIN Lei#,ZHOU Yingmin#,LI Caiwu,et al.Composition and variation of giant pandas gut microbiome between trained, preparatory reintroduction, reintroduction and wild[J].Chinese Journal of Applied & Environmental Biology,2019,25(01):1-10.[doi:10.19675/j.cnki.1006-687x.2018.06007]
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野化培训与放归、野生大熊猫肠道菌群的组成和变化*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年01期
页码:
1-10
栏目:
研究论文
出版日期:
2019-02-25

文章信息/Info

Title:
Composition and variation of giant pandas gut microbiome between trained, preparatory reintroduction, reintroduction and wild
文章编号:
201806007
作者:
晋 蕾1# 周应敏2# 李才武2 吴代福2 李 果2 何永果2 李 蓓1 黄 炎2 张和民2** 邹立扣1**
1四川农业大学资源学院 成都 611130?
2中国大熊猫保护研究中心 卧龙 623006
Author(s):
JIN Lei1# ZHOU Yingmin2# LI Caiwu2 WU Daifu2 LI Guo2 LI Bei1 HUANG Yan2 ZOU Likou1 & ZHANG Hemin2?
1 College of Resources, Sichuan Agricultural University, Chengdu 611130, China?
2 China Conservation and Research Center for the Giant Panda , Wolong 623006, China
关键词:
培训预放归放归野外大熊猫肠道菌群
Keywords:
trained preparatory reintroduction reintroduction wild GPs gut microbiome
DOI:
10.19675/j.cnki.1006-687x.2018.06007
摘要:
追踪采集1只亚成年大熊猫(Ailuropoda melanoleuca)在野化培训(n=4)、预放归(n=4)和放归(n=5)过程的粪样,及5个野生大熊猫粪样,基于高通量测序技术,研究大熊猫肠道细菌、真菌的组成和变化。结果发现,亚成年大熊猫肠道内细菌以 Firmicutes(61.33%)和Proteobacteria(35.09%)门,Clostridium(39.68%)、Escherichia(20.94%)和Leuconostoc(8.75%)属为主。真菌以Ascomycota(81.56%)门,Humicola(26.43%)、Sphaerulina(12.42%)和Aspergillus(4.49%)属为主。预防归大熊猫肠道细菌菌群的组成和放归大熊猫相似,但真菌菌群存在差异。随着培训-放归-野外的过程变化,细菌菌群丰富度和多样性差异不显著(p > 0.05),其中Chao-1指数呈上升趋势,而Shannon指数呈下降趋势。而野外和培训大熊猫肠道真菌菌群的丰富度差异显著(p < 0.05) ,Chao-1和Shannon指数均呈上升趋势。同时发现,在细菌菌群中Leuconostoc的比例持续上升而Acinetobacter和Zea比例持续下降。真菌菌群中Stachybotrys、Fusarium、Retroconis 和Debaryomyces的比例持续上升,Cryptococcus 和Lulwoayna比例持续下降。Clostridium、Bacillus、Sphaerulina和Aspergillus在放归过程中比例最高。本研究结果表明预防归过程中喂食竹子的变化、抓捕和转运中的应激反应等可能导致肠道菌群的变化,且真菌菌群的变化较明显。总体而言,野化培训与放归大熊猫肠道菌群的多样性、丰富度和结构组成逐渐向野生大熊猫趋近,野化培训与放归有利于大熊猫肠道菌群的重建,提高放归大熊猫的存活率。(图6 表2 参35)
Abstract:
To investigate the composition and variation of bacteria and fungi in one juvenile giant pandas (GPs) from trained (n=4), preparatory reintroduction (n=4) to reintroduction (n=5) and 5 feces from wild GPs by using high-throughput sequencing technology and the relationship between this study and GPs survival rate. The result showed that the bacteria mainly in juvenile giant pandas consisted of Firmicutes (61.33%) and Proteobacteria (35.09%), and the fungi mainly consisted of Ascomycota (81.56%) at phyla level. At genus level, the bacteria mainly consisted of Clostridium (39.68%), Escherichia (20.94%) and Leuconostoc (8.75%), the fungi mainly consisted of Humicola (26.43%), Sphaerulina (12.42%) and Aspergillus (4.49%). Simultaneously, the composition of bacteria in preparatory reintroduction GPs were similar with reintroduction GPs, contrarily, the result in fungal communities was opposite. The richness and diversity of bacteria had no significant differences among trained, preparatory reintroduction and wild, while the richness of fungi had significant differences between wild vs trained, and wild vs preparatory reintroduction. However, in bacterial communities, the proportion of Leuconostoc with a continuous increased in these four processes while Acinetobacter and Zea in decreased, and in fungal communities, the proportion of Stachybotrys, Fusarium, Retroconis and Debaryomyces with a continuous increased while Cryptococcus and Lulwoayna in decreased. Besides, the propotion of Clostridium , Bacillus, Sphaerulina and Aspergillus in reintroduction GPs were higher than other groups. Therefore, the study showed that GPs may will stress response to different bamboos feeding, arresting and transferring in preparatory reintroduction process and the variation in fungi was more obviously. In general, the richness, diversity and composition of gut microbiome in trained and reintroduction GPs were approached to wild GPs, gradually. Besides, trained and reintroduction can help the GPs reestablish its gut microbiome and improve its survival rate. ? ? ??

备注/Memo

备注/Memo:
收稿日期:2018-06-05 接受日期 Accepted: 2018-06-27*中国大熊猫保护研究中心项目(CCRCGP181918)、卧龙大熊猫俱乐部项目资助 **通讯作者(E-mail:Wolong_zhm@126.com; zoulikou@sicau.edu.cn)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-07-13