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[1]王 云 彭丽云 孙雪丽 高玉莹 陈晓慧 陈裕坤 林玉玲 赖钟雄**.龙眼Hsf基因家族全基因组鉴定及体胚发生过程中的表达分析*[J].应用与环境生物学报,2019,25(02):1-17.[doi:10.19675/j.cnki.1006-687x.2018.06004]
 WANG Yun,PENG Liyun,SUN Xueli,et al.Genome-wide identification of longan Hsf family members and their functional analysis during somatic embryogenesis in longan*[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):1-17.[doi:10.19675/j.cnki.1006-687x.2018.06004]
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龙眼Hsf基因家族全基因组鉴定及体胚发生过程中的表达分析*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
1-17
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Genome-wide identification of longan Hsf family members and their functional analysis during somatic embryogenesis in longan*
文章编号:
201806004
作者:
王 云 彭丽云 孙雪丽 高玉莹 陈晓慧 陈裕坤 林玉玲 赖钟雄**
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
WANG Yun PENG Liyun SUN Xueli GAO Yuying CHEN Xiaohui LIN Yuling & LAI Zhongxiong**
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
龙眼热激转录因子家族DlHsfs成员鉴定表达分析miRNA预测
Keywords:
Dimocarpus longan Lour. heat shock transcription factor family DlHsfs gene identification expression analysis miRNA prediction
DOI:
10.19675/j.cnki.1006-687x.2018.06004
摘要:
基于龙眼基因组数据库对龙眼Hsfs(DlHsfs)基因家族进行全基因组鉴定,并对其基因结构、启动子作用元件和CpG岛分布情况、编码蛋白的理化性质和进化情况以及它们在不同体胚发生阶段和不同组织器官的表达情况进行研究,同时对靶定DlHsfs的miRNA进行预测分析。结果表明:DlHsf基因家族共20个成员,基因结构高度保守。它们编码的蛋白具有典型的DNA结合域(DBD),且均为不含信号肽的不稳定蛋白。启动子分析发现,该基因家族成员的启动子除典型的热激元件(HSE)外,还含有MYB元件等与逆境胁迫及生长发育相关的元件,DlHsfA1d、DlHsfA9a、DlHsfA9b和DlHsfC1启动子存在典型的CpG岛。进化树分析表明DlHsfs可分为DlHsfA、DlHsfB和DlHsfC三类。qRT-PCR结果表明: DlHsfs在胚性阶段(EC)和球形胚阶段(GE)表达量较高,在不完全胚性紧实结构阶段(ICpEC)表达量较低,在龙眼体胚发育过程中呈现“V”状趋势,表明DlHsfs可能主要在EC、GE阶段发挥作用。此外,DlHsfs家族成员在花芽和花蕾中表达量较高,其次是在果肉中,推测DlHsfs可能与龙眼花发育等生长过程相关。psRNA Target预测结果表明:DlHsfs基因家族受到miRNA调控,且调控方式多样。本研究表明DlHsfs功能多样,可能在龙眼生长发育、胁迫响应、DNA甲基化、组织器官特异表达等生物学过程发挥作用。(图8 表5 参38)
Abstract:
Identifying and investigating the biological functions of the Heat Shock Transcription Factor (DlHsfs) family in Dimocarpus longan Lour. Based on the longan genome database, DlHsf gene family members were screened, analyzed with their gene structure, promoter acting elements and CpG island distribution, physicochemical properties and evolution analysis of their encoded proteins. Their expression in different somatic embryogenesis stages and in different tissues and organs were analyzed by quantitative real-time PCR (qRT-PCR). The miRNA targeting DlHsfs family members were predicted and analysed by psRNA Target. In total, 20 DlHsf gene family members were identified. The structures of DlHsfs were highly conserved. The encoding proteins of DlHsfs were all unstable hydrophobic proteins with DNA-binding domain (DBD) and without signal peptides. In addition, all of their promoters contained a heat-stress-related heat shock elements (HSE) and some stress and growth and development related elements, such as MYB related elements, suggesting DlHsfs to be involved in a variety of stress and growth and development related conditions. There were also typical CpG islands in DlHsfA1d, DlHsfA9a, DlHsfA9b and DlHsfC1 promoters. Phylogenetic relationship analysis showed that the DlHsfs were mainly classified into three categories, i.e. DlHsfA, DlHsfB and DlHsfC. The result of qRT-PCR showed that the expression of DlHsf family members varied at different somatic embryogenesis stages and in different tissues and organs. Their expression levels in the embryogenic callus (EC) and globular embryos (GE) were higher than that in incomplete compact pro-embryogenic cultures (ICpEC). With the development of longan somatic embryos, the expression of DlHsfs showed a “V”-like curve, indicating that DlHsfs may play a major role in the EC and GE stages during somatic embryogenesis. Higher expression levels in flower buds and Alabastrums, followed by flesh indicated that the DlHsfs family members influenced the process of flowering and other growth processes during the growth and development of longan tissues and organs. psRNA Target results indicated that miRNA showed diverse regulations on DlHsfs. DlHsfs family members are versatile and may play a role in longan growth, stress response, DNA methylation and tissue-specific expression, and other important biological functions.

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备注/Memo

备注/Memo:
收稿日期: 2018-06-04 接受日期 Accepted: 2018-08-07
*国家自然科学基金(31572088,31672127);福建省重大专项(2015NZ000-1);福建省自然科学基金杰出青年项目(2015J06004)资助?
**通讯作者(E-mail: Laizx01@163.com)
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更新日期/Last Update: 2018-08-30