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[1]叶义全 罗红艳 刘晓霞 李 茂 曹光球 杨 海 许珊珊**.乙烯参与钾对拟南芥缺铁响应的调控*[J].应用与环境生物学报,2019,25(01):1-13.[doi:10.19675/j.cnki.1006-687x.2018.05021]
 YE Yiquan,LUO Hongyan,LIU Xiaoxia,et al.Ethylene is involved in the regulation of potassium on iron-deficiency-induced responses in Arabidopsis thaniala*[J].Chinese Journal of Applied & Environmental Biology,2019,25(01):1-13.[doi:10.19675/j.cnki.1006-687x.2018.05021]
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乙烯参与钾对拟南芥缺铁响应的调控*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年01期
页码:
1-13
栏目:
研究论文
出版日期:
2019-02-25

文章信息/Info

Title:
Ethylene is involved in the regulation of potassium on iron-deficiency-induced responses in Arabidopsis thaniala*
文章编号:
201805021
作者:
叶义全12 罗红艳12 刘晓霞3 李 茂12 曹光球12 杨 海1 许珊珊12**
1福建农林大学林学院 福州 350002
2国家林业局杉木工程技术研究中心 福州 350002
3浙江省农业技术推广中心,浙江杭州 310020
Author(s):
YE Yiquan12 LUO Hongyan12 LIU Xiaoxia12 LI Mao12 CAO Guangqiu12 Yang Hai1&XU Shanshan12**
1 Forestry College of Fujian Agriculture and Forestry University, Fuzhou 350002, China?
2 Chinese Fir Engineering Technology Research Center, State Forestry Administration, Fuzhou 350002, China?
3 Zhejiang Provincial Agricultural Technology Extension Center, Hangzhou 310020, China
关键词:
缺铁响应缺钾质子乙烯根系质外体
Keywords:
Fe-deficiency-induced response potassium deficiency proton ethylene root apoplast
DOI:
10.19675/j.cnki.1006-687x.2018.05021
摘要:
为了解乙烯是否作为信号分子参与钾对植物耐缺铁响应的调控,以拟南芥为研究材料,分析钾在调控植物缺铁响应过程中根中乙烯含量与缺铁响应的变化及其相互关系。结果表明:(1)与缺铁正常供钾相比,缺铁同时缺钾显著增加根中和根系质外体铁含量,降低植株地上部铁含量,加剧植株地上部缺铁,从而进一步增强根中铁还原酶活性促进铁吸收相关基因表达。(2)在缺铁同时缺钾下添加不同浓度的钾则显著促进根系质外体铁的再利用,降低根中铁和根系质外体铁的含量,增加地上部铁含量,改善植株地上部铁营养状况,从而根系铁还原酶活性。(3)与缺铁正常供钾相比,缺铁和缺钾共同胁迫显著增加根中乙烯含量,在条件下外源添加乙烯合成前体均能进一步增加根中乙烯含量并增强铁还原酶活性,而外源添加乙烯合成抑制剂则进一步降低根中乙烯含量并抑制铁还原酶活性。(4)与对照相比,缺铁和缺钾共同胁迫下乙烯合成基因ACS4、ACS6和ACS7显著上调。综上所述,钾对拟南芥缺铁响应具有重要调控作用,ACS6、ACS4和ACS7基因介导合成的乙烯作为信号分子参与钾对拟南芥耐缺铁响应的调控。(图5 表1 参43)
Abstract:
In order to understand whether ethylene acts as a signal molecule involved in the regulation of potassium (K) on iron (Fe) -deficiency-induced responses. In this study, the changes of both ethylene levels and Fe-deficiency-responses and their relationship were analyzed under Fe and/or K deficiency stresses by using Arabidopsis thaliana. The results showed that (1) Fe and K deficiency (-Fe-K) significantly increased the Fe content both in root and root apoplast, and decreased Fe content in shoots, which aggraveated the Fe deficiency in shoots, thus further increased the ferric-chelate reductase (FCR) activity and Fe-uptake related genes expression as compared with Fe deficiency (-Fe+K). (2) Under Fe and K deficiency stress (-Fe-K), exogenous application of various concentrations of K+ greatly promoted the reutilization of Fe in root apoplast, hence the Fe content in both root and root apoplast were decreased, which in turn increased the Fe content in shoots, ameliorate the Fe deficiency in shoot, and ultimately decreased roots FCR activity. (3) The ethylene levels was significantly increased in roots of –Fe-K treatment as compared with –Fe+K, moreover, we also found that exogenous application of ethylene synthesis precursor greatly increased, while ethylene synthesis inhibitor greatly decreased the FCR activity and ethylene levels under both -Fe+K and –Fe-K stresses. (4) We also observed that the higher accumulation of ethylene in roots under Fe and K deficiency stress was accompanied by the higher expression of ethylene synthesis genes ACS4, ACS6, and ACS7 as compared with control. In conclusion, these results suggested that K plays an important role in regulation of Fe-deficiency-induced responses, ACS4, ACS6, and ACS7 mediated the accumulation of ethylene ultimately act as a signal molecule involved in the regulation of K on Fe-deficiency-induced responses.

备注/Memo

备注/Memo:
收稿日期: 2018-05-15 接受日期 Accepted: 20178-07-02?
*国家重点研发计划项目(2016YFD0600301)和福建省自然科学基金(2017J01608)资助?
**通讯作者(E-mail: shi_xu_1987@163.com)
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更新日期/Last Update: 2018-08-02