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[1]涂德辉李德强宋诗昀黄 富陈光登**.水稻砷耐性差异及砷高耐性材料筛选*[J].应用与环境生物学报,2019,25(01):1-13.[doi:10.19675/j.cnki.1006-687x.2018.04046]
 TU Dehui,LI Deqiang,SONG Shiyun,et al.Differences of arsenic tolerance and screening of high arsenic-tolerant material in Rice (Oryza sativa L.) *[J].Chinese Journal of Applied & Environmental Biology,2019,25(01):1-13.[doi:10.19675/j.cnki.1006-687x.2018.04046]
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水稻砷耐性差异及砷高耐性材料筛选*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年01期
页码:
1-13
栏目:
研究论文
出版日期:
2019-01-30

文章信息/Info

Title:
Differences of arsenic tolerance and screening of high arsenic-tolerant material in Rice (Oryza sativa L.) *
文章编号:
201804046
作者:
涂德辉1李德强2宋诗昀1黄 富2陈光登1**
1.四川农业大学资源学院 成都 6111302.四川农业大学农学院生态农业研究所 成都 6111303.成都土壤肥料测试中心 成都 610041
Author(s):
TU Dehui1 LI Deqiang2 SONG Shiyun1 HUANG Fu2 ZHANG Lan3& CHEN Guangdeng1**
1.College of Resource Science, Sichuan Agricultural University, Chengdu 611130, China;?
2.Institute of Ecology and Agriculture, Sichuan Agricultural University, Chengdu 611130, China?
3.Detection Center for Qualitation and Monitoring of Fertilizer, Chengdu 610041 , China
关键词:
水稻耐性指数砷含量砷积累量
Keywords:
rice (Oryza sativa L.) tolerance index arsenic content arsenic accumulation
DOI:
10.19675/j.cnki.1006-687x.2018.04046
摘要:
【目的】为了探讨不同水稻对砷吸收、积累的差异性,聚类筛选获取砷高耐性材料,为进一步培育砷耐性品种提供材料。【方法】研究以85 份水稻亲本材料为研究对象,通过水培试验,研究砷处理下不同水稻植株生长性状和砷积累特征,比较不同材料的砷耐性和砷积累量差异,并以三种耐性指数为指标,综合筛选砷高耐性材料。【结果】(1) 与对照相比,砷处理下水稻的生物量、总根长和株高受到不同程度的抑制。水稻地上部砷含量和积累量在2 mg/L 砷处理下最大值分别是最小值的7.41倍和18.21倍,而8 mg/L 砷处理下水稻地上部砷含量和积累量最大值分别是最小值的10.01倍和49.90倍,说明材料间耐性差异显著,有利于砷高耐性水稻材料的筛选。(2) 综合三种耐性指数进行聚类分析,得到华航35号、五山丰占 、蒲江抗源-5-2、CHETUMALA-86、雅康2A和雅康3A等6种砷高耐性材料和雅恢2119、Wxj-74、Wxj-380、MR183-2和IR28153等5种砷低耐性材料。在2 mg/L 砷处理下,砷低耐性材料平均砷含量、积累量和生物量分别为高耐性材料3.11倍、1.71倍和0.49倍;8mg/L 砷处理下,砷低耐性材料平均砷含量、积累量和生物量分别为高耐性材料1.85倍、1.34倍和0.77倍,说明高耐性材料较低耐性材料能更好适应砷胁迫。(3) 高砷浓度处理下的水稻地上部平均砷含量和积累量显著高于低砷浓度处理;两类耐性水稻材料在砷处理下其地上部平均砷含量、积累量和生物量差异显著,表现为高耐性材料平均砷含量、积累量显著低于低耐性材料,而生物量显著高于低耐性材料。【结论】本研究在不同砷浓度处理下,根据耐性指数差异进行聚类,得到6种砷高耐性材料,可作为砷抗性育种的亲本材料,为中轻度砷污染农田水稻生产提供砷耐性种质资源。(图2 表4 参39)
Abstract:
In order to investigate the differences in arsenic absorption and accumulation of different rice materials, arsenic high tolerance materials were selected by cluster screening to provide materials for the further cultivation of arsenic tolerance varieties. In this study, based on the 85 rice parental materials with significant genetic differences, the characteristics of plant growth traits and arsenic accumulation were studied by hydroponic culture. Arsenic tolerance and arsenic accumulation differences in different materials were compared, and rice germplasms with high tolerance of arsenic were also identified according to the arsenic tolerance indices. The results indicated that: (1) Compared with the control group, the dry weight (DW), total root length (RL) and plant height (PH) of rice materials were inhibited by different degrees under the stress of arsenic ions. The maximum value of arsenic content and accumulation in rice was 7.41 times and 18.21 times of the minimum value under 2 mg/L arsenic treatment, and 10.01 times and 49.90 times under 8 mg/L arsenic treatment respectively. (2) Six high tolerance of arsenic, Huahang35, Wushanfengzhan, Pujiangkangyuan-5-2, CHETUMALA-86, Yakang2A, Yakang3A and five materials with low tolerance of arsenic, Yahui2119, Wxj-74, Wxj-380, MR183-2, IR28153, were obtained according to the clustering of tolerance index differences. The average arsenic content, accumulation and biomass of arsenic low tolerance materials under 2 mg/L arsenic treatment were 3.11 times, 1.71 times and 0.49 times of arsenic high tolerance materials respectively. The average arsenic content, accumulation and biomass of arsenic low tolerance materials under 8 mg/L arsenic treatment were 1.85 times, 1.34 times and 0.77 times of arsenic high tolerance materials respectively. This indicated that arsenic high tolerance materials were more able to adapt to the arsenic stress. (3) The average arsenic content and accumulation amount of rice under high arsenic concentration treatment was higher than that of low arsenic concentration treatment. The average arsenic content, accumulation and biomass of two tolerant rice materials under arsenic treatment were significantly different, the average arsenic content of the high-tolerance material was significantly lower than that of the low-tolerance material, while the biomass was significantly higher than that of the low-tolerance material. In this study, six arsenic high tolerance materials were obtained by clustering according to the difference of tolerance index under different arsenic concentration treatments, which could be used as a parent material for arsenic resistance breeding to provide arsenic-resistant germplasm resources for rice production on the mild and moderate As-contaminated farmland.

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备注/Memo

备注/Memo:
收稿日期: 2018-04-29 接受日期 Accepted: 2018-06-12?
*国家自然科学基金项目(31401377)和四川省科技计划项目(2017JY0126)资助?
**通讯作者(E-mail:gdchen@sicau.edu.cn)
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更新日期/Last Update: 2018-07-13