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[1]敬双怡 李 岩 于玲红** 杨文焕 朱浩君 李卫平.SMBBR工艺处理生活污水脱氮效能及其微生物多样性*[J].应用与环境生物学报,2018,24(06):1-13.[doi:10.19675/j.cnki.1006-687x.2018.04039]
 JING Shuang-yi,LI Yan,YU Ling-hong**,et al.Analysis of Denitrification Efficiency of SMBBR Process for Domestic Sewage Treatment and Its Microbial Diversity[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-13.[doi:10.19675/j.cnki.1006-687x.2018.04039]
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SMBBR工艺处理生活污水脱氮效能及其微生物多样性*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-13
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Analysis of Denitrification Efficiency of SMBBR Process for Domestic Sewage Treatment and Its Microbial Diversity
文章编号:
201804039
作者:
敬双怡1 李 岩1 于玲红1** 杨文焕1 朱浩君12 李卫平1
1.内蒙古科技大学能源与环境学院 包头 0140102.中丹康灵(北京)生物技术有限公司 北京 100085
Author(s):
JING Shuang-yi1 LI Yan1 YU Ling-hong1**YANG Wen- huan ZHU Hao-jun12&LI Wei-ping1
1. School of Energy and Environment, Inner Mongolia University of Science and Technology , Baotou 014010,China?
2.Sino-Danske Cloning(Beijing) Biological Technology Co., Ltd, Beijing 100085,China
关键词:
特异性流化床生物膜法高通量测序微生物群落多样性环境因子
Keywords:
Special Moving Bed Biofilm Reactor High-Sequencing Microbial community diversity Environmental factors
DOI:
10.19675/j.cnki.1006-687x.2018.04039
摘要:
微生物是生物膜法处理技术的核心,微生物多样性的研究对于生物膜法去除污染物的机理探讨具有重要意义。采用特异性流化床生物膜反应器(Special Moving Bed Biofilm Reactor ,SMBBR)处理城市生活用水,并利用Illumina HiSeq高通量测序技术对各反应器的微生物分布以及菌群与环境因子的相关性进行了研究。结果显示:在水温为20~30 ℃,上清液回流比为150%,DO为4 mg/L,水力停留时间为18 h的条件下,SMBBR对氨氮的去除率高达96.7%;SMBBR的好氧反应器和厌氧反应器的生物膜微生物种群结构组成存在不同,但优势微生物种群均为变形菌门(Proteobacteria)和厚壁菌门(Firmicutes)。在属的水平检测到好氧反硝化菌红细菌(Rhodobacter)、陶厄氏菌属(Thauera),以及氨氧化细菌 ( Ammonia-oxidizing bacteria,AOB) 亚硝化单胞菌(Nitrosomonadales)等。另外, DO是影响微生物群落结构最显著的环境因子。研究表明,环境因子会影响微生物群落替演,好氧反硝化以及氨氧化反应可能是SMBBR工艺中重要的脱氮机制。
Abstract:
Microorganism is the core of biofilm treatment technology, so the study of microbial diversity is of great significance to the mechanism of removing the pollutants by biofilm treatment method. In this paper, the Special Moving Bed Biofilm Reactor (SMBBR) was used to treat urban domestic sewage, and Illumina HiSeq high-throughput sequencing technology was used to study the microorganism distribution and the correlation between microflora and environmental factors in each reactor. The results showed under the conditions that water temperature is 20~30 ℃, reflux ratio of liquid supernatant is 150%, DO is 4 mg/L, the hydraulic retention time (HRT) is 18 h, removal rate of ammonia and nitrogen by SMBBR is as high as 96.7%; the structures of biofilm microbial population in aerobic reactor and anaerobic reactor of SMBBR are different, but the dominant microbial population are Proteobacteria and Firmicutes. Aerobic anti-nitrifying bacteria Rhodobacter, Thauera, and ammonia-oxidizing bacteria Nitrosomonadales were detected at the genus level. In addition, DO was the most significant environmental factor affecting the structure of microflora. The results showed that environmental factors affected microbial community substitution, aerobic denitrification and ammoxidation maybe were i mportant denitrification mechanism in SMBBR.

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备注/Memo

备注/Memo:
收稿日期: 2018-04-25 接受日期: 2018-05-09
*内蒙古科技大学产学研合作培育基金项目(PY-201401)资助?
**通讯作者(E-mail:ylh0730@163.com)
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更新日期/Last Update: 2018-06-05