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[1]鲁帅领 朱 慧 符 波,** 刘宏波,刘 和,等.高温条件下混菌发酵合成气产乙酸及其群落结构*[J].应用与环境生物学报,2019,25(01):1-10.[doi:10.19675/j.cnki.1006-687x.2018.04035]
 LU Shuailing,ZHU Hui,FU Bo**,et al.Thermophilic fermentation of syngas to acetate by mixed culture s and their microbial community analysis*[J].Chinese Journal of Applied & Environmental Biology,2019,25(01):1-10.[doi:10.19675/j.cnki.1006-687x.2018.04035]
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高温条件下混菌发酵合成气产乙酸及其群落结构*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年01期
页码:
1-10
栏目:
研究论文
出版日期:
2019-02-25

文章信息/Info

Title:
Thermophilic fermentation of syngas to acetate by mixed culture s and their microbial community analysis*
文章编号:
201804035
作者:
鲁帅领1 朱 慧1 符 波1 2** 刘宏波1 2 刘 和1 2
1江南大学环境与土木工程学院 江苏省厌氧生物技术重点实验室 无锡 214122?
2江苏省水处理技术与材料协同创新中心 苏州 215009
Author(s):
LU Shuailing1 ZHU Hui 1 FU Bo12** LIU Hongbo12 & LIU He12
1 School of Environmental and Civil Engineering, Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, China ?
2 Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou 215009, China
关键词:
合成气发酵混培物乙酸高温菌微生物群落
Keywords:
syngas fermentation mixed culture acetate t hermophiles microbial community
DOI:
10.19675/j.cnki.1006-687x.2018.04035
摘要:
合成气(主要包括CO、H2和CO2)通过生物转化生产高附加值的生物燃料和化学品已引起人们广泛关注,微生物菌群作为生物转化的酶催化剂对合成气发酵产物组成和效率十分关键。通过富集得到高温条件下分别稳定转化CO、甲酸钠和合成气的厌氧菌群,探究CO与甲酸钠转化菌混培物和合成气转化菌发酵合成气生成乙酸的能力,并分析其微生物群落结构。结果显示,CO-甲酸钠转化菌混培物与合成气转化菌在合成气发酵前期主要进行CO的产氢反应生成H2和CO2以及同型产乙酸反应生成乙酸,CO利用率为100%,CO反应速率分别为6.93和6.34 mmol/(L·d);随后同型产乙酸菌利用H2和CO2继续合成乙酸,两者的乙酸最大累积量分别为9.11和8.01 mmol/L。CO-甲酸钠转化菌混培物主要菌群为Thermoanaerobacterium、Romboutsia、Ruminococcus、Clostridium、Eubacterium、Moorella和Desulfotomaculum属,合成气转化混菌则主要含有Romboutsia、Thermoanaerobacterium、Moorella、Eubacterium、Acetonema和Clostridium属,其中同型产乙酸菌广泛分布于Ruminococcus、Clostridium、Eubacterium、Moorella和Acetonema属。本研究表明复配CO和甲酸钠转化菌可用于合成气高温发酵产乙酸,且转化能力优于合成气转化菌,可为合成气混菌发酵提供微生物资源和技术参考。(图3 表3 参32)
Abstract:
Syngas is a mixture of mainly CO , H2 and CO2, which can be produced from gasification of various organic materials . Syngas fermentation has attracted lots of interests for its ability to produce valuable biofuels and biochemicals. As the biocatalyst for syngas fermentation, microorganisms are important to product profile and conversion efficiency. In this research, thermophilic CO-, formate- and syngas-converting microorganisms were enrichened from cow manure, and the capability for acetate production from syngas by the CO-formate converting bacteria mixture and syngas-converting bacteria as well as their microbial community were analyzed. The results showed that CO-driven hydrogenogenesis and acetogenesis occurred during the initial of syngas fermentation and resulted in the formation of H2+CO2 and acetate , respectively. The CO conversion efficiency was 100 % and CO-converting rates were 6.93 and 6.34 mmol/(L·d) for the CO-formate converting bacteria mixture and syngas-converting bacteria, respectively. Subsequently, H2+CO2 were mainly converted into acetate via acetogenic Wood–Ljungdahl pathway with the final acetate accumulation of 9.11 and 8.01 mmol/L, respectively. Genus Thermoanaerobacterium, Romboutsia, Ruminococcus, Clostridium, Eubacterium, Moorella and Desulfotomaculum dominated in CO-formate converting bacteria mixture , and Romboutsia, Thermoanaerobacterium, Moorella, Eubacterium, Acetonema and Clostridium were main genera in the syngas-converting bacteria, in which Ruminococcus, Clostridium, Eubacterium, Moorella and Acetonema harbor known homoacetogenic species. It is shown that the mixture of CO- and formate-converting bacteria can be used for thermophilic syngas fermentation, and its conversion ability is higher than that of the syngas-converting bacteria. This result provides potential microorganisms resources and technical references for valuable chemicals and bioenergy production by syngas mixed-culture fermentation.

备注/Memo

备注/Memo:
收稿日期: 2018-04-24 接收日期Accepted: 2018-06-19
*国家自然科学基金项目(No. 21206056; 51678280)、江苏省厌氧生物技术重点实验室开放课题基金(No. JKLAB201711)资助?
**通讯作者(E-mail: fubo@jiangnan.edu.cn)
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更新日期/Last Update: 2018-08-02