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[1]崔永亮 李艳梅 李利军 游棵 郑晓琴 涂卫国 王琼瑶 庄启国.钒钛磁铁矿尾矿土壤解钾细菌的多样性及镉对其解钾能力的影响*[J].应用与环境生物学报,2019,25(01):1-12.[doi:10.19675/j.cnki.1006-687x.2018.04016]
 CUI Yongliang,LI Yanmei,LI Lijun,et al.The effect on the potassium-dissolving ability of the potassium-dissolving bacterium by Cadmium in the V-Ti t ailings soil[J].Chinese Journal of Applied & Environmental Biology,2019,25(01):1-12.[doi:10.19675/j.cnki.1006-687x.2018.04016]
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钒钛磁铁矿尾矿土壤解钾细菌的多样性及镉对其解钾能力的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年01期
页码:
1-12
栏目:
研究论文
出版日期:
2019-02-25

文章信息/Info

Title:
The effect on the potassium-dissolving ability of the potassium-dissolving bacterium by Cadmium in the V-Ti t ailings soil
文章编号:
201804016
作者:
崔永亮 李艳梅2 李利军1 游棵2 郑晓琴1 涂卫国1 王琼瑶1 庄启国1
王永志1 吴帆1 余秀梅2** 1 四川省自然资源科学研究院 成都 6100 15
2 四川农业大学资源学院 成都 61 1130
Author(s):
CUI Yongliang1 LI Yanmei 2 LI Lijun1 YOU Ke 2 ZHENG Xiaoqin 1 TU Weiguo1 WANG Qiongyao1 ZHUANG Qiguo1 WANG Yongzhi1 WU Fan1& YU Xiumei1**
1 Sichuan Provincial Academy Of Natural Resource Sciences, Chengdu 610015, China?
?2 Department of resources, Sichuan agricultural university, Wenjiang 611130, China
关键词:
解钾细菌产碱杆菌尾矿土壤
Keywords:
Potassium-dissolving bacteria Cadmium Advenella Tailing soil
DOI:
10.19675/j.cnki.1006-687x.2018.04016
摘要:
解钾菌能分解钾长石、磷灰石等不溶硅铝酸盐无机矿物,使土壤难溶性钾转化为可溶性养分,促进作物生长发育,而钒钛磁铁矿尾矿土壤中解钾细菌的多样性及重金属镉对细菌解钾能力是否有影响尚不清楚。本文从攀枝花钒钛磁铁尾矿土壤中分离纯化细菌资源,通过对细菌解钾能力的定性筛选和定量测定获得高效解钾细菌,利用16S rRNA基因相似性与系统发育分析明确解钾细菌的进化地位,研究不同浓度镉胁迫下高效解钾细菌解钾效率的变化规律,揭示镉对细菌解钾能力的影响。结果显示:通过钾长石粉筛选培养基点接细菌,从136株钒钛磁铁尾矿土壤细菌中筛选出7株解钾细菌,包括根瘤菌(Rhizobium)3株、芽孢杆菌(Bacillus)2株、苍白杆菌(Ochrobactrum)1株、产碱杆菌(Advenella)1株,其在钾长石粉液体培养基中的解钾量为18.63-31.32 mg/L。其中,产碱杆菌KT106解钾能力最强,在培养第6d时解钾量最大;KT106对镉表现出一定的耐受性,其在不同浓度的镉胁迫下解钾效率降低。本研究表明,攀枝花钒钛磁铁尾矿土中仅有5% 细菌具有解钾能力,在较高的重金属镉对细菌解钾能力有抑制作用,这将为解钾促生细菌强化植物修复重金属污染土壤提供科学意见。
Abstract:
Potassium-dissolving bacterium can decompose potassium feldspar, apatite and other insoluble silicon aluminate inorganic mineral . The insoluble potassium in soil is converted into soluble nutrient for promoting crop plant growth and development. However, the diversity of potassium-dissolving bacteria in the V-Ti magnetite tailings soil and the effects of heavy metal cadmium on the potassium-dissolving ability of bacteria remain unclear.Bacteria resources were isolated from the V-Ti magnetite tailing soil in Panzhihua. The high efficient potassium-dissolving bacteria were selected by qualitatively screening and quantificationally measurement of potassium-dissolving activity for isolates. T he evolution status of potassium-dissolving bacteria was determined by using the similarity and phylogenetic analysis of 16S rRNA genes . The potassium-dissolving efficiency change rule of the high efficient potassium-dissolving bacteria was measured in the different concentrations of cadmium stress, revealing the influence of cadmium on potassium-dissolving ability of bacteria.A total of 136 bacteria strains were isolated from the V-Ti magnetite tailings soil in Panzhihua by inoculating bacteria on potassium feldspar . Seven potassium-dissolving bacteria strains were selected among them, including three Rhizobium strains, two Bacillus strains, one Ochrobactrum strains and one Advenella strains. Their soluble potassium content was 18.63-31.32 mg/L in the potassium feldspar liquid medium. Advenella KT106 showed the highest potassium-dissolving capacity among them, and its highest potassium -dissolving content was performed at sixth hour. KT106 showed tolerance against cadmium. The potassium-dissolving efficiency decreased in different concentration of cadmium stress. Only 5% bacteria from the V-Ti magnetite tailings of Panzhihua showed potassium-dissolving ability , but these potassium-dissolving bacteria had certain diversity. Heavy metal cadmium showed inhibiting effect on the potassium-dissolving activity of bacteria. This study provided a scientific basis for the potassium-dissolving bacterium strengthen phytoremediation of heavy metal contaminated soil.

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备注/Memo

备注/Memo:
收稿日期: 2018-04-12 接受日期 Accepted: 2018-05-13
*四川省科技厅重点研发项目(2017SZ0183,2017SZ0087,2017FZ0026)、四川省重大科技专项课题(2017NZDZX0003)和四川省科技创新苗子工程项目(2017RZ0064)资助?
**通讯作者(E-mail: yuxiumeicool@163.com)
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更新日期/Last Update: 2018-07-13