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[1]苟学磊 钟 娟** 周 金燕 舒 丹 赵 杰 杨 杰 谭 红**.卵孢菌素产生菌株角毛壳菌(Chaetomium cupreum)CH21-20发酵培养条件优化*[J].应用与环境生物学报,2018,24(06):1-12.[doi:10.19675/j.cnki.1006-687x.2018.03043]
 GOU Xuelei,ZHONG Juan**,SHU Dan,et al.Fermentation optimization of oosporein producing by Chaetomium cupreum CH21-20 *[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-12.[doi:10.19675/j.cnki.1006-687x.2018.03043]
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卵孢菌素产生菌株角毛壳菌(Chaetomium cupreum)CH21-20发酵培养条件优化*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-12
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Fermentation optimization of oosporein producing by Chaetomium cupreum CH21-20 *
文章编号:
201803043
作者:
苟学磊12 钟 娟1** 周 金燕1 舒 丹1 赵 杰12 杨 杰 1 谭 红1**
1中国科学院成都生物研究所,中国科学院环境与应用微生物重点实验室 成都 610041
2中国科学院大学 北京 100039
Author(s):
GOU Xuelei12 ZHONG Juan1** SHU Dan1 ZHAO Jie12 YANG Jie1 ZHOU Jinyan1 & TAN Hong 1**
1Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology of Chinese Academy of Sciences, Chengdu 610041, China?
?2University of Chinese Academy of Sciences, Beijing 100039, China
关键词:
角毛壳菌卵孢菌素发酵条件培养基成分响应面优化
Keywords:
Chaetomium cupreum oosporein fermentation parameters compositions of culture media response surface methodology
DOI:
10.19675/j.cnki.1006-687x.2018.03043
摘要:
卵孢菌素(oosporein)是具有广谱抗菌活性的二联苯醌类化合物;角毛壳菌(Chaetomium cupreum)CH21-20为本实验室通过遗传改良获得的卵孢菌素高产菌株。利用该菌株进行发酵条件研究,获得其最适发酵条件为接种量(体积分数)3%、初始pH 7.0、装液量为60 mL/250 mL、摇床转速180 r/min、培养温度24 ℃。对培养基碳氮源和无机盐筛选结果表明,15.0 g/L蔗糖、5.0 g/L蛋白粉及0.5 g/L氯化钠能显著提高卵孢菌素产量。以卵孢菌素为响应值,对蔗糖、蛋白粉、氯化钠进行了三因素三水平的Box-Behnken实验设计及响应面法优化研究,得到蔗糖、蛋白粉及氯化钠的最佳配比分别为15.63 g/L、5.22 g/L、0.53 g/L,预测在此发酵条件下卵孢菌素最高产量可达2 547 μg/mL,实验验证得到卵孢菌素产量为2 478 μg/mL,与预测值接近;发酵条件优化后,卵孢菌素产量提高了56.14%。本研究获得了卵孢菌素稳定高产的摇瓶发酵技术参数。(图7 表7 参25)
Abstract:
oosporein is a dibenzoquinone pigment with broad-spectrum antimicrobial activities. The oosporein hyper-producing mutant strain Chaetomium cupreum CH21-20 was obtained by genetically-modified. We studied the effects of the fermentation parameters on the produc tion of oosporein in the fermentation process of CH21-20. The results showed that the optimal fermentation parameters were: 3% as the inoculum size, 7.0 as the initial pH, 60 mL per 250 mL shake flask as the culture volume, 24 ℃ and 180 rpm as the optimal culture temperature and rotation ratio, respectively. The results of the experiments of screening the carbon sources, nitrogen sources and inorganic salts revealed that 15.0 g/L sucrose, 5.0 g/L protein powder and 0.5 g/L sodium chloride had significant influences on the yield of oosporein. According to that, the production of oosporein as the response, a Box- Behnken Design of three factors and three levels was designed. The highest production of oosporein was 2 547 μg/mL when the concentration of sucrose, protein powder and sodium chloride was 15.63 g/L, 5.22 g/L, 0.53 g/L, respectively, after response surface prediction. T he terminal production of oosporein reached 2 478 μg/mL after confirmatory experiment, enhanced almost 56.14% compared to the production before the conditions were optimized. Stable and high-producing fermentation parameters of oosporein were acquired at shake-flask level.

备注/Memo

备注/Memo:
收稿日期: 2018-03-27 接受日期 Accepted: 2018-04-16
*中国科学院环境与应用微生物重点实验室、环境微生物四川省重点实验室开放研究基金(KLEAMCAS201502)资助?
**通讯作者(E-mail: abath@cib.ac.cn, zhongjuan@cib.ac.cn)
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更新日期/Last Update: 2018-07-13