|本期目录/Table of Contents|

[1]冯殿宝 王维红** 王燕杉 苏馈足.以黏土为载体好氧颗粒污泥培养及其对番茄废水处理的研究*[J].应用与环境生物学报,2018,24(06):1-11.[doi:10.19675/j.cnki.1006-687x.2018.03033]
 FENG Dianbao,WANG Weihong**,WANG Yanshan & SU Kuizu.Study on aerobic granular sludge is cultured with clay and its treatment of tomato paste processing wastewater*[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-11.[doi:10.19675/j.cnki.1006-687x.2018.03033]
点击复制

以黏土为载体好氧颗粒污泥培养及其对番茄废水处理的研究*()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-11
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Study on aerobic granular sludge is cultured with clay and its treatment of tomato paste processing wastewater*
文章编号:
201803033
作者:
冯殿宝1 王维红1** 王燕杉1 苏馈足2
1新疆农业大学水利与土木工程学院 乌鲁木齐 830052?
2合肥工业大学土木与水利工程学院 合肥 230009
Author(s):
FENG Dianbao1 WANG Weihong1** WANG Yanshan1 & SU Kuizu 2
1College of Hydraulic and Civil Engineering, Xinjiang Agricultural University, Urumqi 830052, China ?
2School of Civil Engineering, Hefei University of Technology , Hefei 230009, China
关键词:
番茄酱加工废水好氧颗粒污泥黏土高通量测序技术
Keywords:
tomato paste processing wastewater aerobic granular sludge clay high-throughput sequencing
DOI:
10.19675/j.cnki.1006-687x.2018.03033
摘要:
针对新疆番茄酱加工废水排放量大、有机物浓度高等问题,采用好氧颗粒污泥技术。为了研究黏土对于污泥好氧颗粒化进程的影响,在SBR反应器中接种絮体污泥并投加黏土,以人工合成番茄酱加工废水为基质培养好氧颗粒污泥,并采用扫描电镜、激光共聚焦(CLSM)、死活细菌染色以及高通量测序等技术表征活性污泥的颗粒化过程。结果显示,在反应器中投加黏土运行20 d时获得平均粒径0.54 mm左右的好氧颗粒污泥。颗粒污泥成熟后,污泥沉降性能较好,COD、NH4+-N、PO43--P的平均去除率分别达到90%、85%、40%以上。扫描电镜下,颗粒污泥轮廓清晰,结构密实。CLSM显示,α-多糖、β-多糖和蛋白质作为胞外聚合物(EPS)的组成部分在颗粒中分布广泛,贯穿整个颗粒截面。添加黏土培养的颗粒污泥死活细菌染色显示其死细菌数量积累程度更高,活细菌多位于外层并包裹着死细菌。高通量测序结果显示,黏土投加后微生物多样性提高,Shannon指数由4.50增至4.79,动胶菌属和产黄菌属的相对丰度分别为37.87%和8.79%。本研究表明,投加黏土可促进好氧颗粒污泥形成,维持体系稳定运行及有机物降解效果主要源于微生物群落的共同作用。(图8 表1 参25)
Abstract:
Aerobic granular sludge technology was adopted to treat tomato paste processing wastewater in Xinjiang due to its high amount of discharge and high concentration of organics. A sequencing batch reactor (SBR) was operated to investigate the effect of clay augmentation on aerobic granulation. Flocculent sludge and clay were fed into synthetic tomato paste processing wastewater in the SBR. The combined bio-analytical techniques of scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), live/dead staining and high-throughput sequencing were used to characterize the granulation process of activated sludge. As showed by the results, the average particle size of the granules in the clay-added reactor was about 0.54 mm in 20 days. After granular sludge matured, it had a good settling ability, the average removal rate of COD, NH4+-N, PO43--P reached 90%, 85% and 40%, respectively. The SEM showed that the granules had a clear outline and a compacting structure. The CLSM revealed that α-D-glucopyranose polysaccharide, β-D- glucopyranose polysaccharide, and protein, as components of EPS, were widely distributed throughout the whole granule. Live/dead bacteria staining images of the clay-added granular sludge showed a higher accumulation of dead bacteria. The live bacteria were distributed more closely to the edge of the granule while the dead bacteria were wrapped inside. High-throughput sequencing results showed that the microbial diversity increased after the addition of clay, Shannon index increased from 4.50 to 4.79. The relative abundance of the Zoogloea and Flavobacterium were 37.87% and 8.79%, respectively. The study demonstrated that clay could accelerate the formation of aerobic granular sludge , and the effect of maintaining the stability of the system and degrading organic mainly came from the combined action of microbial communities.

相似文献/References:

[1]李军,** 周延年 何梅 王亚宜 韦苏.城市污水处理厂好氧颗粒污泥的特性*[J].应用与环境生物学报,2008,14(05):640.
[2]陈国科,黄钧,毕京芳,等.好氧颗粒污泥耐受高碳氮负荷过程中的群体感应[J].应用与环境生物学报,2014,20(01):73.[doi:10.3724/SP.J.1145.2014.00073]
 CHEN Guoke,HUANG Jun,BI Jingfang,et al.Quorum sensing of aerobic granular sludge tolerating high carbon and nitrogen loads[J].Chinese Journal of Applied & Environmental Biology,2014,20(06):73.[doi:10.3724/SP.J.1145.2014.00073]
[3]王硕,于水利,徐巧,等.好氧颗粒污泥特性、应用及形成机理研究进展[J].应用与环境生物学报,2014,20(04):732.[doi:10.3724/SP.J.1145.2014.01009]
 WANG Shuo,YU Shuili,XU Qiao,et al.Characteristics, application and formation mechanisms of aerobic granular sludge: recent advances[J].Chinese Journal of Applied & Environmental Biology,2014,20(06):732.[doi:10.3724/SP.J.1145.2014.01009]
[4]关梦龙,黄钧,毕京芳,等.好氧颗粒污泥代谢高浓度有机废水的数学模拟[J].应用与环境生物学报,2014,20(06):1063.[doi:10.3724/SP.J.1145.2014.04005]
 GUAN Menglong,HUANG Jun,BI Jingfang,et al.Mathematical simulation of aerobic granular sludge metabolizing high-concentration organic wastewater[J].Chinese Journal of Applied & Environmental Biology,2014,20(06):1063.[doi:10.3724/SP.J.1145.2014.04005]
[5]董晶晶,吴迪,马柯,等.好氧颗粒污泥工艺强化脱氮研究进展[J].应用与环境生物学报,2018,24(01):177.[doi:10.19675/j.cnki.1006-687x.2017.02008]
 DONG Jingjing,WU Di,MA Ke,et al.Review on enhanced denitrification of aerobic granular sludge technology[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):177.[doi:10.19675/j.cnki.1006-687x.2017.02008]

备注/Memo

备注/Memo:
收稿日期: 2018-03-22 接受日期: 2018-04-26
*新疆农业大学研究生科研创新项目(XJGRI2016066)和国家自然科学基金项目(51668062)资助?
**通讯作者(E-mail:wangwh95515@163.com)
点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-06-05