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[1]徐珍媚,邓光兵** 张海莉 梁俊俊 龙 海 余懋群.基于BSA分析定位控制西藏大麦侧小穗发育的基因*[J].应用与环境生物学报,2018,24(06):1-14.[doi:10.19675/j.cnki.1006-687x.2018.03032]
 XU Zhenmei,Deng Guangbing,Zhang Haili,et al.Mapping loci controlling lateral spike development in Tibetan barley by bulked segregant analysis *[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-14.[doi:10.19675/j.cnki.1006-687x.2018.03032]
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基于BSA分析定位控制西藏大麦侧小穗发育的基因*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-14
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Mapping loci controlling lateral spike development in Tibetan barley by bulked segregant analysis *
文章编号:
201803032
作者:
徐珍媚1 2 邓光兵1** 张海莉1 梁俊俊1 龙 海1 余懋群1
1中国科学院成都生物研究所 成都 610041?
2中国科学院大学 北京 100049
Author(s):
XU Zhenmei12 Deng Guangbing1 Zhang Haili1 Liang Junjun1 Long Hai1 Yu Maoqun 1
1Chinese Academy of Sciences, Chengdu Institute of Biology, Chengdu 610041, China ?
2University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
西藏大麦棱数性状BSA(集群分离法)基因定位
Keywords:
Tibetan barley row type bulked segregant analysis (BSA) SLAF-seq gene mapping
DOI:
10.19675/j.cnki.1006-687x.2018.03032
摘要:
大麦侧小穗结实与否产生了二棱/六棱性状,从而显著影响其籽粒产量,因此大麦二棱到六棱的变化具有显著驯化特征。青藏高原野生和栽培大麦资源丰富,被认为是栽培大麦的驯化和遗传多样性中心之一。为了进一步了解大麦棱数调控的遗传基础以及西藏栽培大麦驯化的过程,我们以西藏野生二棱大麦和六棱大麦地方品种为亲本构建遗传分离群体,遗传分析发现二棱性状受单个显性基因位点控制。通过集群分离(BSA,Bulked segregant analysis )法分别建立含有22个F2单株的二棱混池和六棱混池,基于SLAF-seq(Specific-locus amplified fragment sequencing)技术共获得456 691个SLAF标签,通过SNP-index和ED两种关联算法交集得到3个与棱数性状相关的侯选区域,总长度为53.84 Mb,包含536个基因,其中能分别被GO、KEGG和COG三个数据库注释的基因有413、189和160个基因。上述研究结果实现了对控制西藏大麦侧小穗发育性状相关基因的初步定位,可为后续目标基因的精细定位和克隆提供理论参考。
Abstract:
Fertility of the lateral spikelets determines the two-rowed or six-rowed spikes in barley ( Hordeum vulgare L.) , which results in significantly different grain yields. The change of row type from two-rowed to six-rowed shows remarkable domestication characteristics. Qinghai-Tibet plateau has abundant resources of wild and cultivated barley, considered as one of the centers of domestication and genetic diversity of cultivated barley. In order to get a primary understanding of the genetic basis on lateral spike development regulation and the domestication process in cultivated Tibetan barley, an F2 segregation population was constructed by crossing the two-rowed wild barley accession ZYM0083 and six-rowed landraces Linzhiheiliuleng. The genetic analysis showed that the row type was controlled by a single gene. By using specific-locus amplified fragment sequencing (SLAF-seq) based on the methods of BSA, two DNA pools from 22 two-rowed spike individuals and 22 six-rowed spike individuals of F2 population were constru cted and sequenced. A total of 456 691 SLAF tags were obtained. By adopting ED and SNP-index for association analysis, three candidate regions with 53.84 Mb interval containing 536 genes were obtained. 413, 189 and 160 annotated genes were acquired by GO, KEGG and COG library, respectively. Loci that control lateral spike development in Tibetan barley were primarily mapped by SLAF-seq, and the results presented in this study will facilitate the fine mapping and cloning of target gene.

备注/Memo

备注/Memo:
收稿日期: 2018-03-21 接受日期: 2018-04-10
*国家自然科学基金面上项目(31771790)、四川省科技支撑计划-四川省农作物分子育种平台项目(2016NZ0103)和四川省育种公关项目(2016NYZ0030)资助?
**通讯作者(E-mail: denggb@cib.ac.cn )
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更新日期/Last Update: 2018-05-02