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[1]廖 丹 黄华斌 庄峙厦 洪有为**.多环芳烃(PAHs)污染对滨海湿地入侵植物互花米草的影响[J].应用与环境生物学报,2018,24(04):1-16.[doi:10.19675/j.cnki.1006-687x.2018.03021]
 LIAO Dan,HUANG Huabin,ZHUANG Shixia,et al.A comprehensive study of the impact of polycyclic aromatic hydrocarbons (PAHs) contamination on invasive plant Spartina alterniflora in coastal wetland[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):1-16.[doi:10.19675/j.cnki.1006-687x.2018.03021]
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多环芳烃(PAHs)污染对滨海湿地入侵植物互花米草的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
1-16
栏目:
研究论文
出版日期:
2018-08-25

文章信息/Info

Title:
A comprehensive study of the impact of polycyclic aromatic hydrocarbons (PAHs) contamination on invasive plant Spartina alterniflora in coastal wetland
文章编号:
201803021
作者:
廖 丹1 黄华斌1 庄峙厦1 洪有为2**
1厦门华厦学院检验科学与技术系 厦门 361024?
2中国科学院城市环境研究所城市环境与健康重点实验室 厦门 361021
Author(s):
LIAO Dan1 HUANG Huabin1 ZHUANG Shixia1 HONG Youwei 2**
1Department of Science and Technology for Inspection of Xiamen Huaxia University, Xiamen 3610242Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021
关键词:
多环芳烃互花米草根内细菌PAHs-环羟基双加氧酶荧光定量PCR
Keywords:
PAHsSpartina alternifloraendophytic bacteria PAH-ring hydroxylating dioxygenases Real-Time PCR
DOI:
10.19675/j.cnki.1006-687x.2018.03021
摘要:
滨海湿地生态系统正遭受着人为活动或自然因素的威胁,本研究旨在探究多环芳烃(PAHs)污染对滨海湿地入侵植物互花米草根际、根内微生物及其在植物-微生物联合修复PAHs潜力的影响。设置含有不同浓度菲和芘的沉积物处理,通过盆栽实验对互花米草幼苗进行暴露。结果表明,培养70天后,菲和芘的去除率分别为13-36%和11-30%;菲处理的互花米草根际沉积物中脱氢酶活性显著高于对照(P < 0.05),非根际沉积物多酚氧化酶活性则降低10%。基于磷脂脂肪酸(PLFA)分析,菲处理显著降低了根际沉积物微生物量(P< 0.05),尤其是革兰氏阴性菌下降了24%;而芘处理对脱氢酶、多酚氧化酶以及总微生物量的影响相对较小。对于100 mg/kg菲处理,使得根际与根内革兰氏阴性菌PAHs-环羟基双加氧酶基因(PAH-RHDα-GN)丰度比对照组分别增加了100倍和3倍;而对于100 mg/kg芘处理,根际与非根际沉积物中革兰氏阳性菌PAHs-环羟基双加氧酶基因(PAH-RHDα-GP)丰度显著升高(p<0.05)。相关研究结果表明,入侵植物互花米草对PAHs污染存在明显的响应,其根内细菌在植物-微生物联合修复PAHs污染具有重要的作用。(图5表4参37)
Abstract:
The functions of coastal wetland ecosystem are increasingly threatening due to several anthropogenic and natural factors. This study aimed to investigate the effects of polycyclic aromatic hydrocarbons (PAHs) on invasive plants (Spartina alterniflora) uptake, rhizophere, endophytic bacteria , and phytoremediation potentials of contaminated sediments. Based on pot experiments, Spartina alterniflora was selected and cultivated in different concentrations of phenanthrene (PHE) and pyrene (PYR) contaminated sediments (for 70 days). The results indicated that the amount of PHE removed from the sediments ranged from 13 to 36%, while PYR ranged from 11 to 30%. In rhizophere sediment, dehydrogenase activities were significantly (P<0.05) enhanced by higher concentration of PHE treatments, while polyphenol oxidase activities were prohibited more than 10% in non- rhizophere sediment. Compare with the control, PHE treatments had also significantly (P<0.05) lower total microbial biomass, especially for gram-negative bacteria this decrease was more than 24%. However, the PYR treatments had little effect on the dehydrogenase, polyphenol oxidase and total PLFA biomass. The greatest abundance of PAH-ring hydroxylating dioxygenases isolated from gram negative bacteria (PAH-RHDα-GN) of rhizoplane and endophyte in roots were found at high concentration of PHE and increased by more than 100- and 3-fold, respectively. These results suggested that PAH pollution would result in the comprehensive effect on Spartina alterniflora, whose endophytic bacteria might play important roles in the phytoremediation potential of PAH contaminated sediments.

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备注/Memo

备注/Memo:
收稿日期: 2018-03-15 接受日期: 2018-04-11
*国家自然科学基金项目(41006064)、福建省教育厅中青年科技项目(JAT170817)、黄土与第四纪地质国家重点实验室开放基金(SKLLQG1607)和2017年厦门市留学人员专项科研项目资助
**通讯作者(E-mail: ywhong@iue.ac.cn)
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更新日期/Last Update: 2018-06-05