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[1]柴淑丽 陈亚菲 樊晓旭 杜泽萱 王 丽**.地钱 (Marchantia polymorpha)雌雄配子体对酸雨胁迫的耐受性差异*[J].应用与环境生物学报,2018,24(06):1-13.[doi:10.19675/j.cnki.1006-687x.2018.03010]
 CHAI Shuli,CHEN Yafei,FAN Xiaoxu,et al.Gender differences of Marchantia polymorpha in tolerance to acid rain stress[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-13.[doi:10.19675/j.cnki.1006-687x.2018.03010]
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地钱 (Marchantia polymorpha)雌雄配子体对酸雨胁迫的耐受性差异*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-13
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Gender differences of Marchantia polymorpha in tolerance to acid rain stress
文章编号:
201803010
作者:
柴淑丽 陈亚菲 樊晓旭 杜泽萱 王 丽**
四川大学生命科学学院 成都 610064
Author(s):
CHAI Shuli CHEN Yafei FAN Xiaoxu DU Zexuan &WANG Li **
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610064, China
关键词:
酸雨胁迫地钱雌雄异株叶绿素荧光参数抗氧化物酶
Keywords:
AR stress Marchantia polymorpha dioecy fluorescence kinetic parameters antioxidase
DOI:
10.19675/j.cnki.1006-687x.2018.03010
摘要:
酸雨是抑制植物生长以及破坏植被覆盖的主要环境因子。在长期的进化过程中,植物进化出了精细而又复杂的防御机制来应对酸雨胁迫。植物可以在个体,细胞及生理生化等多个水平上对酸雨胁迫作出响应。然而,关于雌雄异株的苔藓植物对酸雨胁迫的响应以及雌雄配子体之间的耐受性差异目前还不清楚。在本研究中,我们用不同pH的模拟酸雨对地钱 ( Marchantia polymorpha ) 雌雄配子体进行处理,并对两者之间的耐受性差异进行比较。结果表明:(1)与对照(pH6.8)相比,酸雨胁迫可对地钱造成可见伤害症状;存活率、叶绿素含量、PSII原初光能转化效率(Fv/Fm)和实际光化学量子产量(Yeild)均降低,而丙二醛含量(MDA)、抗氧化物酶(POD 、SOD、CAT)活性、基础荧光(F0)和非光化学淬灭系数(NPQ)均升高。(2)与对照相比,中( pH4.0 )、重度( pH2.5 )酸雨胁迫可对地钱的上述测定值造成显著影响;(3)中度酸雨胁迫下,雌雄之间的存活率、MDA、POD、SOD、光合色素含量、Fv/Fm值、F0 、NPQ值和Yeild值的变化均达到了显著性差异水平,其中雌性的存活率、POD、SOD 、Fv/Fm值和Yeild值均低于雄性。综上所述,酸雨胁迫会对地钱的植株、细胞膜系统以及光合系统造成不同程度的损伤,且雌雄配子体之间存在差异。此外,在酸雨胁迫下雄配子体抗褪绿和抗氧化的能力要高于雌配子体,表现出对酸雨胁迫更强的耐受性。该结论为深入研究植物的性别生态适应性以及酸雨对植被的影响提供了重要的理论依据。图3表1参52
Abstract:
Acid rain (AR) is a major environmental factor s that significantly limit plants growth and lead to vegetation destruction. Plants have evolved elaborate and intricate mechanisms to achieve optimal adaption to AR stress. Plants can respond to AR at multiple levels, including individual, cellular and physiological and biochemical processes. However, the responses of dioecious bryophytes to AR stress and tolerance between female and male gametophytes are not fully understood. In this study, Marchantia polymorpha were treated with different pH levels of AR, and gender difference of tolerance to AR were compared. The results showed as follows: ( 1 ) Compared with the control of pH 6.8, AR caused the visible symptoms of injury to female and male gametophytes; survival rate ( % ) , chlorophyll content, the maximum efficiency of PSII photochemistry ( Fv/Fm ) and actual PSII photochemical quantum yield ( Y ield ) decreased, while malondialdehyde ( MDA ) content, antioxidant enzyme activities ( POD, SOD , CAT ), initial fluorescence ( F 0 ) and non-photochemical quenching coefficient ( NPQ ) increased. ( 2 ) Compared with the control , the above-mentioned parameters under moderate level ( pH4.0 ) and severe level ( pH2.5 ) of AR were significantly different. ( 3 ) Under moderate level , female gametophyte displayed a lower survival rate, antioxidant enzyme activities, chlorophyll content , fluorescence parameters and a higher MDA content than that of male. In summary, AR causes severe damages to individual, photosynthetic system and cellular membrane system of M. polymorpha, and the damage degree is different between female and male gametophyte. Furthermore, male gametophyte has higher resistance to chlorosis and antioxidation than female gametophyte under AR stress, which indicate higher tolerance to AR stress . These conclusions will lead to a better understanding of ecological adaptability of dioecious plants and a daptation AR effects on vegetation.

备注/Memo

备注/Memo:
收稿日期: 2018-03-08 接受日期: 2018-04-08
*四川省科技厅项目(2017SZ01860)资助
**通讯作者(E-mail: yzxj@vip.163.com)
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更新日期/Last Update: 2018-06-05