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[1]佘晨兴,王静 苏玉萍,等.三十六脚湖水库沉积物聚磷菌多样性及群落组成[J].应用与环境生物学报,2018,24(06):1-12.[doi:10.19675/j.cnki.1006-687x.2018.03001]
 SHE Chenxing,WANG Jing,et al.Diversity and community composition of phosphorus accumulating organisms(PAOs) in the sediments of Sanshiliujiao Lake reservoir *[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-12.[doi:10.19675/j.cnki.1006-687x.2018.03001]
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三十六脚湖水库沉积物聚磷菌多样性及群落组成()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-12
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Diversity and community composition of phosphorus accumulating organisms(PAOs) in the sediments of Sanshiliujiao Lake reservoir *
文章编号:
201803001
作者:
佘晨兴1 2 王静1 苏玉萍1 2 林婉珍1 2 兰瑞芳1 2 刘键熙1 2 林佳1 2
1福建师范大学环境科学与工程学院 福州 350007?
2福建省污染控制与资源循环利用重点实验室 福州 350007
Author(s):
SHE Chenxing1 2 WANG Jing1 SU Yuping1 2 LIN Wanzhen1 2 LAN Ruifang1 2 LIU Jianxi1 2 & LIN Jia1 2
1College of Environmental Science and Engineering, Fujian Normal University , Fuzhou 350007, China.?
2Fujian Key Laboratory of Pollution Control and REsource Reuse, Fuzhou 350007, China.
关键词:
水库沉积物聚磷菌多样性T-RFLP群落组成
Keywords:
reservoir sediment phosphorus accumulating organismsPAOs diversity terminal restriction fragment length polymorphism (T-RFLP) community composition
DOI:
10.19675/j.cnki.1006-687x.2018.03001
摘要:
深入研究聚磷菌的多样性及群落组成,有助于揭示水库沉积物磷代谢循环的微生物学机制。采用末端限制性片段长度多态性(Terminal restriction fragment length polymorphism ,T-RFLP)技术结合克隆文库构建,分析了福建省平潭岛三十六角湖水库夏冬季特定样点沉积物聚磷菌多样性及群落组成。结果表明,三十六脚湖沉积物中夏季聚磷菌的多样性明显高于冬季,夏季优势聚磷菌末端限制性片段(Terminal restriction fragment ,T-RF)集中在200-300 bp,而冬季聚磷菌T-RF片段集中在150-200 bp。克隆测序和系统发育分析表明,三十六脚湖水库沉积物样品中检测到优势的聚磷菌类群为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和酸杆菌门(Acidobacteria)。夏季丰度大于10%优势的菌属为厌氧粘细菌(Anaeromyxobacter)、固氮螺菌(Azospirillum)、聚磷小月菌(Microlunatus phosphovorus)和伯克氏菌属( Burkholderia),而冬季丰度大于14%优势的菌属主要有厌氧粘细菌(Anaeromyxobacter)、甲基杆菌属(Methylobacterium)、Solibacter属、固氮螺菌(Azospirillum)和聚磷小月菌(Microlunatus phosphovorus)。本研究表明,三十六脚湖沉积物中聚磷菌多样性及群落组成均呈现出一定的季节变化特征,沉积物各形态磷对聚磷菌多样性有一定影响,且与Fe/Al-P相关性最显著,这为今后揭示水库沉积物磷代谢循环的微生物学机制提供了科学基础。(图6 表3 参37)
Abstract:
The study of the diversity and community composition of phosphorus accumulating organisms(PAOs) in the sediments contributes to reveal microbial mechanism of phosphorus metabolic cycle in reservoirs. The diversity and community composition of PAOs in the sediments of the Sanshiliujiao Lake reservoirs in the Pingtan island of Fujian province were investigated by Terminal restriction fragment length polymorphism(T-RFLP)methods combined with construction of clone libraries. The diversity of PAOs in summer samples was significantly higher than that in winter. The dominant terminal restriction fragments (T-RFs ) of PAOs concentrated in the range of 200 bp to 300 bp in summer, while concentrated in the range 150 bp to 200 bp in winter. Cloning sequencing and phylogenetic analysis showed that mainly phylum of PAOs in the sediment of the Sanshiliujiao Lake reservoir were concentrated in the Proteobacteria, Actinobacteria and Acidobacteria. Anaeromyxobacter, Azospirillum , Microlunatus phosphovorus and Burkholderia were dominant genera existed in summer samples. Anaeromyxobacter, Methylobacterium, Solibacter, Azospirillum and Microlunatus phosphovorus were the dominant genera in sediment samples in winter. Results in this study demonstrated that the diversity and community composition of PAOs in the sediments of Sanshiliujiao Lake reservoirs showed certain seasonal characteristics.Various forms of phosphorus also demonstrated the influence on the diversity of PAOs , especially Fe/Al-P.The?res earch will laid?the?scientific?foundation for reveal microbial mechanism of phosphorus metabolic cycle in the sediments of reservoirs in the future.

相似文献/References:

[1]徐立杰,郭春艳,彭永臻,等.强化生物除磷系统的微生物学及生化特性研究进展[J].应用与环境生物学报,2011,17(03):427.[doi:10.3724/SP.J.1145.2011.00427]
 XU Lijie,GUO Chunyan,PENG Yongzhen,et al.Review on the Microbiological and Biochemical Characteristics of Enhanced Biological Phosphorus Removal System[J].Chinese Journal of Applied & Environmental Biology,2011,17(06):427.[doi:10.3724/SP.J.1145.2011.00427]
[2]伍思宇 周志如 尤 青 张文飞 徐 达 李特伦 姚 琦 张起畅 吴红萍 王锐萍?.海南东寨港红树林聚磷菌的筛选及其特性?[J].应用与环境生物学报,2016,22(03):397.[doi:10.3724/SP.J.1145.2015.11026]
 WU Siyu,ZHOU Zhiru,YOU Qing,et al.Screening and characterization of soil phosphate accumulating bacteria from Dongzhaigang mangrove wetlands in Hainan, China*[J].Chinese Journal of Applied & Environmental Biology,2016,22(06):397.[doi:10.3724/SP.J.1145.2015.11026]

备注/Memo

备注/Memo:
收稿日期: 2018-03-01 接受日期: 2018-04-26
*国家自然科学基金面上项目(41573075)、福建省自然科学基金面上项目(2018J01742)和福建省中青年教师教育科研项目(JAT170132)资助?
**通讯作者(E-mail: ypsu@fjnu.edu.cn)
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更新日期/Last Update: 2018-06-05