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[1]黄艳蒙 张爱平 曹 沁 李 东,** 刘晓风,**.甲基营养型芽孢杆菌H0对羽毛产寡肽的降解特征*[J].应用与环境生物学报,2018,24(06):1-9.[doi:10.19675/j.cnki.1006-687x.2018.01047]
 HUANG Yanmeng,ZHANG Aiping,C AO Qin,et al.Research on oligopeptide production by feather-degrading bacterium Bacillus methylotrophicus H0 *[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-9.[doi:10.19675/j.cnki.1006-687x.2018.01047]
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甲基营养型芽孢杆菌H0对羽毛产寡肽的降解特征*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-9
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Research on oligopeptide production by feather-degrading bacterium Bacillus methylotrophicus H0 *
文章编号:
201801047
作者:
黄艳蒙12 张爱平12 曹 沁1 李 东1** 刘晓风1**
1中国科学院成都生物研究所 成都 610041
2中国科学院大学 北京 100049
Author(s):
HUANG Yanmeng12 ZHANG Aiping12 C AO Qin1 LI Dong1**& LIU Xiaofeng1 **
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China ??
2 University of Chinese Academy of sciences, Beijing 100000, China
关键词:
羽毛角蛋白分离鉴定甲基营养型芽孢杆菌生物降解寡肽
Keywords:
Feather keratin Isolation and identification Bacillus methylotrophicus biodegradation oligopeptide
DOI:
10.19675/j.cnki.1006-687x.2018.01047
摘要:
寡肽具有较高的营养价值和多种生理功能,筛选高产寡肽的羽毛降解菌株,并将其用于羽毛寡肽的生产,对高效利用废弃羽毛资源,提高产品附加值具有重要意义。本研究以寡肽产率为评价指标,从16株羽毛降解菌中分离到一株高产寡肽的羽毛降解菌H0,通过形态观察、生理鉴定和系统发育分析,初步鉴定菌株H0为甲基营养型芽孢杆菌(Bacillus methylotrophicus)。对其降解羽毛产寡肽特性和羽毛寡肽特征进行初步探究,发现该菌在最佳初始pH(11)和最佳温度(40 ℃)下发酵72 h,羽毛几乎完全降解,寡肽产率达38.19%(占可溶性总肽的67.53%),同时检测到11.11%的游离氨基酸。液质联用(LC-MS/MS)分析结果表明所产寡肽的分子量主要分布在1300 Da以下,且主要是由5-10个氨基酸组成的短肽,富含支链氨基酸,推测其是羽毛降解液呈现抗氧化活性的主要原因。本研究表明甲基营养型芽孢杆菌H0具有较强的降解羽毛产寡肽能力,可为羽毛寡肽产品的开发提供优质微生物资源和科学依据。(图8 表2 参22)
Abstract:
Objectives: Because of high nutritive value of oligopeptides and waste of feather resources, this study was aimed at screening efficient strains to degrade feather rapidly and produce value-added oligopeptides with high yield . Methods: By assessing the potential yield of oligopeptides, a promising s train H0 was selected from 16 feather-degrading microorganisms. To identify the strain, the morphological, physiological characteristics and 16S rRNA gene sequence analysis were carried out. The single factor experiment was used for promoting the feather degradation and oligopeptide production, and the characteristics of produced oligopeptides were also analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).Results: The strain was identified as Bacillus methylotrophicus. The optimal initial pH and temperature for oligopeptide production were 11 and 40 °C, respectively. After 72 h fermentation under the optimal conditions, the feathers were almost completely degraded, and 38.19% oligopeptides (accounting for 67.53% of the total soluble peptides) and 11.11% free amino acids were obtained. LC-MS/MS analysis indicated that the produced oligopeptides were mainly short peptides containing 5-10 amino acids, with a molecular mass less than 1300 Da. moreover, the peptides were abundant with branched-chain amino acids , which might be responsible for the antioxidant property of feather hydrolysate. Conclusions: The results demonstrate the great capability of Bacillus methylotrophicus H0 in feather degradation and oligopeptide production. This research provides a high-quality microorganism resource and scientific basis for the development of feather oligopeptide products.

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备注/Memo

备注/Memo:
收稿日期: 2018-01-30 接受日期: 2018-03-12
*中国科学院科技服务网络计划(STS)项目、中国科学院战略生物资源服务网络计划项目(ZSYS-009)、中国科学院青年创新促进会资助(2017423)和成都市科技惠民项目(2016-HM02-00092-SF)资助?
**通讯作者(E-mail: lidong@cib.ac.cn, liuxf@cib.ac.cn)
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更新日期/Last Update: 2018-04-16