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[1]高晓静 张昊琳 桑羽希 蔡盼盼 张国庆 陈青君**.利用杂草培养料栽培双孢蘑菇的可行性分析[J].应用与环境生物学报,2018,24(06):1-12.[doi:10.19675/j.cnki.1006-687x.2018.01033]
 GAO Xiaojing,ZHANG Haolin,SANG Yuxi,et al.Utilization of Reservoir Weeds for mushroom (Agaricus bisporus) production[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-12.[doi:10.19675/j.cnki.1006-687x.2018.01033]
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利用杂草培养料栽培双孢蘑菇的可行性分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-12
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Utilization of Reservoir Weeds for mushroom (Agaricus bisporus) production
文章编号:
201801033
作者:
高晓静1 张昊琳1 桑羽希1 蔡盼盼1 张国庆2 陈青君1**
1北京农学院植物科学技术学院农业应用新技术北京市重点实验室 北京 102206?
2北京农学院生物科学与工程学院农业部都市农业(北方)
Author(s):
GAO Xiaojing 1 ZHANG Haolin 1 SANG Yuxi 1 CAI Panpan1 ZHANG Guoqing 2 CHEN Qingjun 1**
1Department of Plant Science Technology, Beijing University of Agricultural, Beijing 102206,China ?
2College of Biological Sciences and Engineering, Beijing University of Agricultural, Beijing 102206, China
关键词:
培养料木质纤维素木质纤维素降解酶细菌菌群Illumina 测序
Keywords:
compost lignocellulose lignocellulase bacterial community Illumina sequencing
DOI:
10.19675/j.cnki.1006-687x.2018.01033
摘要:
充分利用当地杂草资源生产双孢蘑菇对于降低生产成本和环境保护意义重大。本研究在密云库区杂草多样性调查和配方工艺调整的基础上,对三个批次库区杂草的双孢蘑菇(Agaricus bisporus)生产进行了全程监测,包括堆肥期和出菇期取样,培养料理化性质、物质变化、相关降解酶活性和培养料发酵过程中细菌菌群变化,统计产量等。结果表明,库区杂草种类繁多,株高茎粗,茎杆坚硬紧实,打捆后的杂草饱和吸水量76.78 % 。堆肥期培养料的含水量、含碳量和碳氮比逐渐下降,出菇期变化不大;培养料的含氮量在培养料一次发酵结束(PI)均有下降的现象,二次发酵结束(PII)含氮量升高。培养料二次发酵过程中纤维素和半纤维素的利用率均介于40%-60%之间;木质素利用率介于20%-30%。在双孢蘑菇菌丝生长和出菇过程中木质素的利用率为16%-21%。培养料纤维素和半纤维素的含量变化与相关降解酶变化情况相一致。堆肥期样品Illumina测序得到432595条有效序列,序列平均长度为441bp;分类分析表明,堆肥时期优势菌群为拟杆菌门(Bacteroidetes)的普氏菌属(Prevotella),厚壁菌门(Firmicutes)的芽孢杆菌属(Bacillus),栖热菌门(Deinococcus-Thermus)的Thermus属、Truepera属、Caldicoprobacter属(一种木聚糖降解菌),放线菌门(Actinobacteria)热多孢菌属(Thermopolyspora)、变形菌门(Proteobacteria)的假黄单胞菌属(Pseudoxanthomonas)。三个批次产量在17.1-19.7 kg/m2范围,库区杂草取代麦草进行双孢蘑菇工厂化生产是可行的。 (图3 表4 参30)
Abstract:
Objectives: Making full use of local weed resources to produce Agaricus bisporus is of great significance in reducing production cost and protecting environment.Methods: In this paper, three trials experiments were conducted on the basis of weed diversity investigation around the Miyun reservoir and the adjustment of formulation and technology in the industrial production of Agaricus bisporus. Compost samples from different phases of composting and cultivation stages were collected for the determination of physical-chemical properties, lignocellulose content, lignocellulolytic enzyme activities, and bacterial communities by 16s rRNA gene sequencing . The Yield of m ushroom in different trials was also counted.Results: The results showed that there were many kinds of reservoir weeds with hard and coarse stems. The saturated moisture of weeds was 76.78% after baling. The water content, carbon content and C/N ratio of samples decreased gradually during composting, but had little change during cultivation. The nitrogen content decreased at end of Phase I and increased at the end of Phase II. During composting, the lose rates of hemicellulose and cellulose were both between 40% and 60%, and the loss rate of lignin was between 20% and 30%. During cultivation, the loss rate of lignin was between 16% and 21%. The changes in the content of cellulose and hemicellulose of compost were consistent with that of the activity of related degradation enzymes. For samples from three trials during composting, a total of 432,595 valid sequences were obtained by Illumina sequencing, and the average length of the sequences was 441bp. Taxonomic analysis showed that the dominant bacteria were Prevotella ( Bacteroidetes), Bacillus ( Firmicutes), Thermus, Truepera, Caldicoprobacter (Deinococcus-Thermus), Thermopolyspora (Actinobacteria), Pseudoxanthomonas (Proteobacteria).Conclusions: The yield of three trials was in the range of 17.1-19.7 kg/m 2. It is feasible to use reservoir weeds compost instead of wheat straw compost for cultivation of Agaricus bisporus.

相似文献/References:

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 LI Ke,JIN Yanling,et al.Progress in Research of Key Techniques for Ethanol Production from Lignocellulose[J].Chinese Journal of Applied & Environmental Biology,2008,14(06):877.[doi:10.3724/SP.J.1145.2008.00877]
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备注/Memo

备注/Memo:
收稿日期: 2018-01-22 接受日期: 2018-02-11?
*北京市农委农业科技项目( PXM 2016_014207_000052)、2017年现代农业产业技术体系北京市食用菌创新团队建设经费(PXM2017_014207_000058)资助?
**通讯作者(E-mail:cqj3305@126.com)
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更新日期/Last Update: 2018-04-16