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[1]郝莎莎 卫旭芳 侯智霞** 李守科.文冠果根系瘤状物可培养内生细菌的特性*[J].应用与环境生物学报,2018,24(05):1-12.[doi:10.3724/SP.J.1145.2018.01016]
 HAO Shasha,WEI Xufang,HOU Zhixia**,et al.Characteristics analysis of culturable endophytic bacteria of Xanthoceras sorbifolia Bunge with tumor root*[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1-12.[doi:10.3724/SP.J.1145.2018.01016]
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文冠果根系瘤状物可培养内生细菌的特性*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1-12
栏目:
研究论文
出版日期:
2018-10-25

文章信息/Info

Title:
Characteristics analysis of culturable endophytic bacteria of Xanthoceras sorbifolia Bunge with tumor root*
文章编号:
201801016
作者:
郝莎莎1 卫旭芳1 侯智霞1** 李守科2
1北京林业大学林学院省部共建森林培育与保护重点实验室 北京 100083?
2山东沃奇农业开发有限公司 山东 262100
Author(s):
HAO Shasha1 WEI Xufang1 HOU Zhixia1** LI Shouke2
1College of Forestry, Beijing Forestry University, Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing 100083, China?
?2Shandong Worth Agricultural Development Co.,Ltd., Shandong 262100, China
关键词:
文冠果根系瘤状物内生细菌铁载体IAA溶磷
Keywords:
Xanthoceras sorbifolia tumor root endophytic bacteria siderophore IAA phosphate solubilizing
DOI:
10.3724/SP.J.1145.2018.01016
摘要:
针对具有根系瘤状物着生的文冠果植株,纯化并鉴定了由根系瘤状物分离出来的内生细菌,分别采用CAS平板检测法、Salkowski比色法、平板溶磷圈法以及钼锑抗比色法分析了内生细菌的产铁载体能力、产IAA能力以及溶磷能力。通过形态和分子鉴定,分离纯化出了瘤状物特有的9个菌株,分别命名为XSB1-XSB9,其中6株属于芽孢杆菌属(Bacillus),2株属于短芽孢杆菌属(Brevibacillus),1株属于假单胞菌属(Pseudomonas)。这9个菌株均能产生铁载体,其中XSB3,XSB4,XSB8,XSB9为极高产量菌株,XSB5,XSB6为高产量菌株,高产量以上的菌株为XSB3,XSB4,XSB5,XSB6,XSB8,XSB9,占供试菌株的66.7%;9个菌株均能产生IAA,不加色氨酸时其产IAA的能力为10-35mg/L,加色氨酸后,产IAA的能力为15-50mg/L,且菌株XSB2,XSB3,XSB4,XSB5,XSB9产IAA能力与不加色氨酸时相比差异显著(P<0.05),说明IAA的合成可能是以色氨酸为前体的色氨酸合成途径;这9个菌株均有一定的溶磷能力,菌株XSB1,XSB2,XSB4,XSB5溶磷量极显著高于其他菌株(P<0.01),其溶磷量在50-90mg/L之间,溶磷能力可提高19-29倍。综合分析认为菌株XSB4,XSB5产铁载体、产IAA以及溶磷能力都较强,值得作为备选菌株进行进一步的促生能力和促生机理研究。该研究结果可为文冠果根际微生物的开发利用、抗性机制、提高文冠果的栽培水平等方面的研究和实践奠定基础。(图6表2参40)
Abstract:
Objectives:Xanthoceras sorbifolia Bunge with the tumor root were discovered, the endophytic bacteria which isolated from the tumor root were purified and identified. This paper aimed to study the characteristics of endophytic bacteria.Methods: The CAS detection plate, Salkowski colorimetric, phosphate solubilizing circle and molybdenum antimony spectophotometric were adopted to analyze endophytic bacteria ability which produced siderophore, secreted IAA and dissolved phosphorus. Results: Strains were isolated from the tumor root by morphological and molecular identification, they were named as XSB1-XSB9, of which 6 strains belonged to Bacillus, 2 strains belonged to Brevibacillus and 1 strain belonged to Pseudomonas. All of the 9 strains can produce siderophore, of which strains XSB3, XSB4, XSB8 and XSB9 were extremely high yielding strains, and strains XSB5 and XSB6 were high yielding strains. The strains with high yield were XSB3, XSB4, XSB5, XSB6, XSB8 and XSB9, accounting for 66.7% of the tested strains; 9 strains can secret IAA, the concentration of IAA secreted by strains containing tryptophan was between 15-50mg/L, and the concentration of the one without tryptophan was between 10-35mg/L. The IAA ability of strains XSB2, XSB3, XSB4, XSB5 and XSB9 were significantly different (P <0.05) after adding tryptophan, These results indicated that the synthesis of IAA may be the tryptophan synthesis pathway using tryptophan as precursor; 9 strains all had some ability to dissolve phosphorus.The content of phosphate solubilizing strains XSB1, XSB2, XSB4 and XSB5 were significantly higher than those of other strains (P<0.01), and the content of phosphate solubilizing was between 50-90 mg/L,and the ability of dissolving phosphate could be increased by 19-29 times.Conclusions: Strains XSB4 and XSB5 which produced siderophore, secreted IAA and dissolved phosphorus capacity are strong, worth as a candidate strain for further ability to promote growth. The results will lay the foundation for the development and utilization of rhizosphere microbes , the resistance mechanisms and cultivation level of the Xanthoceras sorbifolia.

备注/Memo

备注/Memo:
收稿日期: 2018-01-14 接受日期: 2018-02-01
*中央高校基本科研业务费专项资金资助(2015ZCQ-LX-02)和国家国际科技合作专项项目(2014DFA31140)资助?
**通讯作者(E-mail: hzxn2004@163.com)
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更新日期/Last Update: 2018-03-19