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[1]魏春梅,栾威,代娅,等.比较转录组研究钛离子对紫花苜蓿基因表达的影响[J].应用与环境生物学报,2019,25(01):117-127.[doi:10.19675/j.cnki.1006-687x.2018.04028]
 WEI Chunmei,LUAN Wei,et al.Effects of titanium ions on gene expression of Medicago sativa by comparative transcriptome analysis[J].Chinese Journal of Applied & Environmental Biology,2019,25(01):117-127.[doi:10.19675/j.cnki.1006-687x.2018.04028]
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比较转录组研究钛离子对紫花苜蓿基因表达的影响
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年01期
页码:
117-127
栏目:
研究论文
出版日期:
2019-02-25

文章信息/Info

Title:
Effects of titanium ions on gene expression of Medicago sativa by comparative transcriptome analysis
作者:
魏春梅 栾威 代娅 王明秀 陶向 王艳 李彩侠 马欣荣
1中国科学院成都生物研究所 成都 610041 2中国科学院大学 北京 100049
Author(s):
WEI Chunmei1 2 LUAN Wei1 2 DAI Ya1 WANG Mingxiu1 TAO Xiang1 WANG Yan1 LI Caixia1 & MA Xinrong1**
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2 University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
钛离子紫花苜蓿基因差异表达核糖体蛋白热激蛋白抗病相关基因WRKY基因
Keywords:
titanium ion Medicago sativa gene differential expression ribosomal protein heat shock protein pathogenesis-related gene WRKY gene
分类号:
S482.8
DOI:
10.19675/j.cnki.1006-687x.2018.04028
摘要:
钛离子可显著促进植物生长,增加作物产量. 以紫花苜蓿(Medicago sativa)为材料,用钛离子水溶液(0,8 mg/L)喷施植株叶面,24 h后取样进行Illumina高通量RNA-Seq测序,研究钛离子对紫花苜蓿叶片基因表达的影响,在转录水平上研究钛离子作用的分子机理. 以蒺藜苜蓿(Medicago truncatula)为参考基因组进行比对,结果显示,共获得28 437个注释基因. 差异表达分析表明,与对照相比,8 mg/L钛离子处理引起大量基因表达发生变化,482个差异表达的基因中247个上调表达,235个下调表达. GO和KEGG注释结果显示,大量核糖体(18)、热激蛋白(7)、转录因子WRKY(5)类编码基因上调表达;尽管抗病相关基因上调的个数(11)低于下调的(22),但这些抗病相关基因的总体表达量,FPKM从12 563上升至16 197. 下调的基因主要有细胞色素P450家族基因(上调2个,下调7个). 推测钛离子作为一种胁迫信号刺激紫花苜蓿产生应答,引起基因表达发生变化,上调了与生长和胁迫适应相关基因的表达,进而促进植物生长和提高对逆境的耐受性. 本研究为揭示钛离子调节植物生长的机制打下基础,为进一步钛离子制剂在农业生产中的应用提供指导. (图5 表4 参46)
Abstract:
Titanium ions promote plant growth and increase crop yield significantly, but the mechanism remains an enigma. In this study, we determined the effect of titanium ions on gene expression in Medicago sativa using Illumina high-throughput RNA-sequencing. After 24 h of foliar-sprayed with a titanium ion solution (0, 8 mg/L), the leaves of M. sativa were sampled for transcriptome sequencing. The resulting sequences were mapped onto the reference genome of Medicago truncatula, leading to the annotation of 28 437 genes. The differential expression analysis showed that 482 genes expressed differentially, including 247 up-regulated and 235 down-regulated upon 8 mg/L titanium ion solution compared with the control. Further analysis showed that the genes encoding ribosomal proteins (18), heat shock proteins (7) and transcription factor WRKYs (5) were mainly up-regulated. Although the number of up-regulated genes (11) associated with disease resistance was less than that of down-regulated ones (22), the total expression abundance of these genes increased from 12 563 to 16 197 FPKM. In addition, the down-regulated genes mainly included cytochrome P450 family genes (2 up-regulated, 7 down-regulated). It was speculated that titanium ions act as a stress signal to stimulate plants to respond, resulting in gene expression alterations that promote plant growth and improves the adaptability to adverse conditions. This study lays the foundation for revealing the regulatory mechanism of titanium ions on plant growth and provides a basis for its further application in agricultural production.

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更新日期/Last Update: 2019-02-25