|本期目录/Table of Contents|

[1]郭玉琼 黄道斌 常笑君 朱 晨 李小桢 赖钟雄**.铁观音茶树体胚发生及其内源激素变化研究*[J].应用与环境生物学报,2018,24(05):1-15.[doi:10.3724/SP.J.1145.2017.12027]
 GUO Yuqiong,HUANG Daobin,CHANG Xiaojun,et al.Studies on somatic embryogenesis and the changes of endogenous hormones in Camellia sinensis cv.Tieguanyin*[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1-15.[doi:10.3724/SP.J.1145.2017.12027]
点击复制

铁观音茶树体胚发生及其内源激素变化研究*()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1-15
栏目:
研究论文
出版日期:
2018-10-25

文章信息/Info

Title:
Studies on somatic embryogenesis and the changes of endogenous hormones in Camellia sinensis cv.Tieguanyin*
文章编号:
201712027
作者:
郭玉琼1 黄道斌12 常笑君12 朱 晨1 李小桢1 赖钟雄12**
1福建农林大学园艺学院 福州 350002
2福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
GUO Yuqiong1 HUANG Daobin12 CHANG Xiaojun12 ZHU Chen1 LI Xiaozhen1 & LAI Zhongxiong12**
1College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
铁观音茶树体胚发生内源激素
Keywords:
Tieguanyin Camellia sinensis somatic embryogenesis endogenous hormones
DOI:
10.3724/SP.J.1145.2017.12027
摘要:
本研究以乌龙茶品种铁观音茶树的未成熟胚为供试材料,建立高效的茶树体胚发生再生体系,同时采用HPLC方法分析茶树体胚发生过程中各个不同发育阶段的内源激素含量变化趋势。结果表明,诱导茶树体胚发生的培养基为:改良MS培养基+2 mg/L BA+0.2 mg/L IBA+甜菜碱800 mg/L,体胚增殖系统维持途径的培养基为:改良MS培养基+3 mg/L 肉桂酸+20 g/L 蔗糖+10 g/L 山梨醇。当3%蔗糖与2%山梨醇组合使用时,诱导产生子叶形胚的百分率明显提高。将诱导产生的子叶形胚转接至添加5 %蔗糖与5 % PEG的无激素MS培养基中,经过40 d左右培养,体胚能正常成熟。成熟体胚接种在附加2 mg/L BA与0.1 mg/L IBA的MS培养基上可以萌发成苗。在体胚发生早期,ZT和GA3含量均是先下降,在子叶形胚时期降至最低值,到体胎发育后期的成熟胚与萌发阶段呈明显上升趋势;ABA含量由球形胚到子叶形胚阶段一直呈现下降趋势,到成熟胚阶段时,出现一个明显的峰值;球形胚中的IAA含量显著高于其它阶段,之后迅速降低,波动变化。ABA/IAA的比值先增大,然后减小;ABA/GA3的比值体胚发育前期维持在较高水平,到成熟萌发阶段下降;球形胚阶段IAA/ZT的比值高于其它各个阶段。本研究表明,茶树不同发育阶段胚胎的体胚诱导率不同,与生理状态及其内源激素动态变化有关。同时茶树中内源激素含量和比例在体细胞胚胎发育过程中有显著的变化,这种变化在不同胚胎发育阶段起着特定的调节作用。(图8 表13 参38)
Abstract:
Objectives: In this experiment, the immature embryos of oolong tea cultivar ‘Tieguanyin’ were used as materials to establish an efficient regeneration system of tea somatic embryogenesis.Methods: HPLC method was used to analyzed the change trend of endogenous hormone content in different developmental stages of tea somatic embryogenesis. Results: The medium of inducing somatic embryogenesis was modified MS medium supplemented with 2 mg/L BA, 0.2 mg/L IBA and 800 mg/L betaine, and the medium for sustaining somatic embryo proliferation system was modified MS medium supplemented with 3 mg/L cinnamic acid, 20 g/L sucrose and 10 g/L sorbitol. The percentage of cotyledon embryos was improved obviously when 3% sucrose was used in combination with 2% sorbitol. The induced cotyledon embryos were transferred to the medium supplemented with 5% sucrose and 5% PEG without phytohormones, and the somatic embryos could be mature after 40 d’s culture. Mature embryos could be induced to germinate and regenerate on the MS medium supplemented with 2 mg/L BA and 0.1 mg/L IBA. At the stage of early somatic embryogenesis, the content of ZT and GA3 both first decreased and reached the lowest value in the cotyledon embryo period, and the content of ZT showed a significant upward trend in the mature embryo and germination embryo period at the stage of post-embryonic development. The content of ABA decreased from the globular embryos to the cotyledon embryos, and reached the peak at the mature embryos stage. The content of IAA in the globular embryo was significantly higher than that in the other stages, and then decreased rapidly and showed small fluctuation. The ratio of ABA/IAA increased first and then decreased. At the early stage of somatic embryogenesis, the ratio of ABA/GA3 maintained at a high level, and decreased in the mature embryo and germination embryo period. The ratio of IAA/ZT of the globular embryos was higher than that in other stages.Conclusions: This study shows that the induction rate of embryo in different developmental stages was different, which was related to the physiological state and the dynamics of endogenous hormones in the tea cultivar ‘Tieguanyin’. At the same time, the content and proportion of endogenous hormones changed significantly during the development of somatic embryos. This change played a specific regulatory role in different stages of embryonic development in the tea.

相似文献/References:

[1]黄安平,韩宝瑜,包小村.茶刺蛾危害后茶树挥发性有机化合物释放变化[J].应用与环境生物学报,2011,17(06):819.[doi:10.3724/SP.J.1145.2011.00819]
 HUANG Anping,HAN Baoyu,BAO Xiaocun.Change in Volatile Organic Compounds from Camellia sinensis (L.) O. Kuntze Damaged by Iragoides fasciata Moore (Lepidoptera: Eucleidae)[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):819.[doi:10.3724/SP.J.1145.2011.00819]
[2]王海斌** 叶江华 陈晓婷 贾小丽 孔祥海.连作茶树根际土壤酸度对土壤微生物的影响[J].应用与环境生物学报,2016,22(03):480.[doi:10.3724/SP.J.1145.2015.09019]
 WANG Haibin**,YE Jianghua,CHEN Xiaoting,et al.Effect on soil microbes of the rhizospheric soil acidity of tea tree continuous cropping*[J].Chinese Journal of Applied & Environmental Biology,2016,22(05):480.[doi:10.3724/SP.J.1145.2015.09019]
[3]孙平,章国营,向萍,等.茶树中莽草酸途径DHD/SDH基因的表达调控[J].应用与环境生物学报,2018,24(02):322.[doi:10.19675/j.cnki.1006-687x.2017.05014]
 SUN Ping,ZHANG Guoying,XIANG Ping,et al.Expression and regulation of the shikimic acid pathway gene DHD/SDH in tea plant (Camellia sinensis)[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):322.[doi:10.19675/j.cnki.1006-687x.2017.05014]
[4]王海斌,** 陈晓婷 丁力 邱丰艳 叶江华 贾小丽 孔祥海.不同树龄茶树根际土壤细菌多样性的T-RFLP分析[J].应用与环境生物学报,2018,24(04):1.[doi:10.3724/SP.J.1145.2017.10003]
 WANG Haibin,**,CHEN Xiaoting,et al.Using T-RFLP technology to analyze bacteria diversity in rhizospheric soil of different age tea tree*[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1.[doi:10.3724/SP.J.1145.2017.10003]
[5]郭玉琼 王 仲 朱 晨 赵姗姗 张舒婷 常笑君 李小桢 傅海峰 林玉玲 赖钟雄**.茶树CSD1基因及其启动子克隆与低温胁迫下的表达分析*[J].应用与环境生物学报,2018,24(05):1.[doi:10.19675/j.cnki.1006-687x.2018.02021]
 GUO Yuqiong,WANG Zhong,ZHU Chen,et al.Cloning and expression analysis of copper/zinc-superoxide dismutase 1 gene and its promotor under low temperature stress in Camellia sinensis*[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1.[doi:10.19675/j.cnki.1006-687x.2018.02021]
[6]王海斌,**陈晓婷丁 力叶江华贾小丽孔祥海何海斌.福建省安溪县茶园土壤酸化对茶树产量及品质的影响*[J].应用与环境生物学报,2018,24(06):1.[doi:10.19675/j.cnki.1006-687x.2017.12008]
 WANG Haibin,**,CHEN Xiaoting,et al.Effect of soil acidification on yield and quality of tea tree in tea plantations from Anxi county, Fujian province *[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1.[doi:10.19675/j.cnki.1006-687x.2017.12008]
[7]张 玥 胡雲飞 王树茂 柯子星 高水练 林金科**.茶园年限对根际土壤真菌群落结构及多样性的影响*[J].应用与环境生物学报,2018,24(06):1.[doi:10.19675/j.cnki.1006-687x.2018.04011]
 ZHANG Yue,HU Yunfei,WANG Shumao,et al.Effect of the structure and diversity of fungal community in rhizosphere soil from different ages of tea garden *[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1.[doi:10.19675/j.cnki.1006-687x.2018.04011]

备注/Memo

备注/Memo:
收稿日期: 2017-12-11 接受日期: 2018-01-09
*福建省重大科技专项(2015NZ0002-1)、福建省教育厅资助项目(JA15143)、福建农林大学科技创新专项基金项目(CXZX2017164)资助?
**通讯作者(E-mail:Laizx01@163.com)
点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-02-12