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[1]林 旭 何洁 冯守帅** 杨海麟** 黄 兴 吴泉钱 顾利星.硫氧化菌Halothiobacillus neapolitanus筛选、鉴定及其脱硫性能*[J].应用与环境生物学报,2018,24(05):1-9.[doi:10.3724/SP.J.1145.2017.12021]
 LIN Xu,FENG Shoushuai**,YANG Hailing**,et al.Isolationidentification and biodesufurization performance research of Halothiobacillus neapolitanus[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1-9.[doi:10.3724/SP.J.1145.2017.12021]
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硫氧化菌Halothiobacillus neapolitanus筛选、鉴定及其脱硫性能*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1-9
栏目:
研究论文
出版日期:
2018-10-25

文章信息/Info

Title:
Isolationidentification and biodesufurization performance research of Halothiobacillus neapolitanus
文章编号:
201712021
作者:
林 旭1 何洁1 冯守帅1** 杨海麟1** 黄 兴2 吴泉钱2 顾利星2
1江南大学生物工程学院 教育部工业微生物技术重点实验室 无锡 214122?
2无锡市城市环境卫生有限公司 无锡 214122
Author(s):
LIN Xu1 FENG Shoushuai1** YANG Hailing1** HUAGN Xing2 WU Quanqian2 GU Lixing2
1School of Biotechnology, Jiangnan University, Key Laboratory of Industrial Biotechnology, Ministry of Education, Wuxi, 214122, China.2Wuxi City Environmental Sanitation Co. Ltd, Wuxi, 214122, China.
关键词:
Halothiobacillus neapolitanus内循环气升式反应器生物脱硫耐受性
Keywords:
Halothiobacillus neapolitanus Internal airlift loop reactor Biodesulfurization Tolerance
DOI:
10.3724/SP.J.1145.2017.12021
摘要:
硫氧化菌是生物脱硫的关键因素,因此筛选一株耐受性强、脱硫效率高的菌株具有重要意义。本研究以硫代硫酸钠为能源底物,从无锡市某污水处理厂硝化污泥中分离到一株硫氧化菌株,通过菌落形态、TEM电镜观察,并结合16S rRNA测序以及系统进化树分类等分子生物学分析,鉴定该菌为那不勒斯硫杆菌(Halothiobacillus neapolitanus),命名为LJN1-3。确定该菌的最适脱硫条件为pH 6.8,温度30 °C,在该条件下,适量葡萄糖、蔗糖、乙醇等有机物以及Ni2+可刺激细胞生长,该菌对酸类和Mn2+、Pb2+、Zn2+、Cd2+等金属离子呈现一定耐受性。该菌在内循环气升式反应器脱硫性能分析表明,其最大比生长速率和最大比消耗速率分别为0.38 h-1和6.5×10-4 g cells-1 h-1,24 h内硫代硫酸钠消耗率达99%,此时单质硫浓度0.88 g/L,呈现较强的脱硫潜力。SEM分析形成的单质硫表面呈现粗糙、不规则的形貌。本研究表明,H.neapolitanus LJN1-3 硫代硫酸钠去除率高且具有较强的耐受能力,在生物脱硫等领域具有一定的应用价值。
Abstract:
Desulfurization bacteria is the key factor of biodesulfurization, thus it is significant to screen a strain that has high tolerance and efficiency in desulfurization. A sulfur oxidizing bacteria strain named LJN1-3 was isolated from an industrial nitrification sludge using thiosulfate as the sole energy. Based on the colony morphologic determination, TEM observation, 16S rDNA gene sequences and phylogenetic tree analysis, this strain was identified as Halothiobacillus neapolitanus. Under the optimum desulfurize conditions (pH 6.8 and 30 °C). Cell growth was promoted with low-carbon organic compounds (glucose, sucrose, ethanol) and Ni2+, while the strain presented high tolerance to acids and metal ions including Mn2+, Pb2+, Zn2+ and Cd 2+. The desulfurization experiment was carried out in an internal airlift loop reactor, the maximum specific growth rate and consumption ratio of strain LJN1-3 reached 0.38 h-1 and 6.5×10 -4 g cells-1 h-1, respectively. After 24 h, the thiosulfate removal ratio and the yield of elemental sulfur reached 99% and 0.88 g/L, respectively. Furthermore, SEM observation of elemental sulfur showed a rough and irregular surface in granular sulfur . Results in this study demonstrated that H.neapolitanus LJN1-3 has application potential in the fields of biological desulfurization based on its high sodium thiosulfate removing ability and high tolerance to acids and metal ions.

备注/Memo

备注/Memo:
收稿日期: 2017-12-08 接受日期: 2018-01-15
*国家自然科学基金项目(21606110,21776113)、江苏省自然科学基金(No. BK20150133)和江苏省科技项目社会发展面上项目(BE2017625)资助?
**通讯作者(E-mail: 19891996@sina.com)
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更新日期/Last Update: 2018-02-12