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[1]梁安洁 龙 凤 洪 滔 林勇明 陈灿 谢安强 吴承祯,洪 伟 李 键 **.槲皮黄素-3-α-阿拉伯糖苷胁迫下内生真菌对木麻黄幼苗根系形态和生理特性的影响*[J].应用与环境生物学报,2018,24(04):1-14.[doi:10.3724/SP.J.1145.2017.12017]
 LIANG Anjie,LONG Feng,HONG Tao,et al.Effects of endophyte fungi on morphological and physiological characteristics of Casuarina equisetifolia seedlings roots under quercetin-3-α-arabinoside stress*[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):1-14.[doi:10.3724/SP.J.1145.2017.12017]
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槲皮黄素-3-α-阿拉伯糖苷胁迫下内生真菌对木麻黄幼苗根系形态和生理特性的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
1-14
栏目:
研究论文
出版日期:
2018-08-25

文章信息/Info

Title:
Effects of endophyte fungi on morphological and physiological characteristics of Casuarina equisetifolia seedlings roots under quercetin-3-α-arabinoside stress*
文章编号:
201712017
作者:
梁安洁1 龙 凤1 洪 滔1 林勇明1 陈灿1 谢安强1 吴承祯1 2 洪 伟1 李 键1 **
1福建农林大学林学院 福州 350002?
2武夷学院生态与资源工程学院 南平 354300
Author(s):
LIANG Anjie1 LONG Feng1 HONG Tao1 LIN Yongming1 CHEN Can1 XIE Anqiang1 WU Chengzhen1 2 HONG Wei1 & LI Jian1**
1 College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China2 College of Ecology and Resource Engineering, Wuyi University, Nanping 354300, China
关键词:
化感胁迫内生真菌木麻黄根系形态生理特性
Keywords:
allelochemical stress endophyte Casuarina equisetifolia L. root morphology physiological characteristics
DOI:
10.3724/SP.J.1145.2017.12017
摘要:
采用水培的方式,测定不同浓度化感物质槲皮黄素-3-α-阿拉伯糖苷(Q3A)胁迫对感染和未感染内生真菌的木麻黄幼苗的根系形态和生理特性的影响。结果表明:不同浓度Q3A胁迫下,感染内生真菌能够有限缓解化感物质的胁迫,在中轻度胁迫(50、25、12.5 mg·L -1)下有明显的效果,表现在与木麻黄EF相比,木麻黄EI根系生物量、根系总长、根表面积显著增加;根系活力显著提升,根系渗透调节物质除可溶性蛋白含量显著增加外,可溶性糖、游离脯氨酸含量显著降低;根系抗氧化系统除谷胱甘肽还原酶(GR)活性显著降低,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、愈创木酚过氧化物酶(GPX)、抗坏血酸过氧化物酶(APX)、还原型抗坏血酸(AsA)和还原型谷胱甘肽(GSH)含量均能显著增强;过氧化氢(H2O2)、羟自由基(OH·)含量、超氧阴离子()产生速率和膜脂过氧化产物丙二醛(MDA)含量也显著降低;内生真菌缓解化感物质胁迫的有效浓度与木麻黄根系能够耐受的化感物质胁迫浓度存在一定相似性。表明内生真菌能在一定条件下缓解不同浓度化感物质槲皮黄素-3-α-阿拉伯糖苷胁迫对木麻黄幼苗根系生长的抑制和氧化损伤,提升了木麻黄根系的抗逆性,在木麻黄根系适应化感胁迫的调节中起关键作用。(图3表3参54)
Abstract:
The effects of different concentrations of quercetin-3- α-arabinoside (Q3A) on the root morphology and physiological characteristics of Casuarina equisetifolia seedlings infected and not infected with endophytic fungi were determined by hydroponics. The results showed that endophytic fungi could alleviate the stress of allelochemical under the stress of Q3A at different concentrations, with significant effects under slight allelochemical stress (50, 25 and 12.5 mg ? L -1). Compared with EF, the root biomass, root length, root surface area and root activity increased significantly, and the contents of soluble sugar and free proline in root osmotic adjustment substances significantly decreased. Endophyte infection significantly decreased the activities of glutathione reductase (GR), the activitiy of protective enzymes like superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX) and ascorbate peroxidase APX, AsA and GSH were significantly increased. The content of H2O2, OH ?, production rate of and malondialdehyde (MDA) content also significantly reduced; The effective concentrations of endophyte fungi alleviate allelochemical stress were similar to those of allelopathic substances tolerant to the root system of C. equisetifolia. Endophyte fungi could alleviate the inhibition of root growth and oxidative damage of C. equisetifolia seedlings under different concentrations of quercetin-3-α-arabinoside stress, enhance the resistance of C. equisetifolia, and play a key role in the C. equisetifolia root to adapt to allelochemical stress.

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备注/Memo

备注/Memo:
收稿日期:2017-12-07 ? 接受日期: 2018-01-07
*国家自然科学基金项目(31400533)、福建省自然科学基金项目 (2015J01607) 和福建农林大学科技创新基金(CXZX2016055)资助?
**通讯作者(E-mail: hmilycau@163.com)
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更新日期/Last Update: 2018-01-10