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[1]顾敏芬 何畅 黄玉彪 黄鹤勇 孟祥天 王芳 蒋新 张银萍**.扫描/透射电子显微技术在土壤环境微生物相互作用的超微结构研究中的应用进展*[J].应用与环境生物学报,2018,24(05):1-9.[doi:10.3724/SP.J.1145.2017.11027]
 HE Chang,GU Minfen,HUANG Yubiao,et al.Application of scanning electron microscopy and transmission electron microscopy in studying ultra-structure interactions between soil and microorganisms *[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1-9.[doi:10.3724/SP.J.1145.2017.11027]
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扫描/透射电子显微技术在土壤环境微生物相互作用的超微结构研究中的应用进展*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1-9
栏目:
综 述
出版日期:
2018-10-25

文章信息/Info

Title:
Application of scanning electron microscopy and transmission electron microscopy in studying ultra-structure interactions between soil and microorganisms *
文章编号:
201711027
作者:
顾敏芬1 何畅1 黄玉彪1 黄鹤勇1 孟祥天1 王芳2 蒋新2 张银萍1**
1南京师范大学分析测试中心南京 210043?
2中国科学院南京土壤研究所南京210009
Author(s):
HE Chang1 GU Minfen1 HUANG Yubiao1 HUNAG Heyong1 MENG Xiangtian1 WANG Fang2 JIANG Xin2&ZHANG Yinping1**
1 Nanjing Normal UniversityAnalysis and Testing CenterNanjing 210043 ?
2 Institute of Soil ScienceChinese Academy of SciencesNanjing 210009
关键词:
微生物扫描电镜透射电镜
Keywords:
Bacteria scanning electron microscopy (SEM) transmission electron microscopy (TEM) soil interface
DOI:
10.3724/SP.J.1145.2017.11027
摘要:
超微结构技术与土壤和微生物等学科的交叉融合,使得相关研究的尺度向着更直观与更微观的两极方向延伸。微生物与土壤组分的相互作用贯穿于整个土壤多相微界面体系,是土壤中最具活力的因子,深刻影响着环境污染物的迁移转化及归趋行为。扫描和透射电子显微技术的结合能够将土壤与微生物组成的高活性超微界面体系可视化,是当今土壤界面化学研究的前沿方向。本文介绍了扫描和透射电子显微技术结合能谱、高角度环形暗场像、电子衍射分析等手段的基本原理和其应用于环境微生物领域的制样要求;综述了应用扫描/透射电子显微技术研究环境胁迫下微生物的形态变化特征、微生物与土壤固相组分的作用、微生物与微生物之间交互作用的超微结构特征,以及结构和形貌观测及化学成分分析;通过扫描/透射电子显微技术揭示土壤微生物与污染物的作用机制,跟踪环境污染物的转化和迁移特征。已开展的研究发现,微生物容易附生于土壤颗粒表面,且通过调整自身细胞表面结构,改变自身转运有机物的通道,从而代谢污染物;共培养模式具有协同效应,在降解有毒有机污染物和处理废弃物方面效率更高,从而加速土壤中污染物的转化与降解过程。此外,本文还总结了通过扫描/透射电子显微技术如何深入挖掘有生命土壤的超微界面作用机理和展望了扫描/透射电子显微技术未来的可能研究方向,期望能进一步促进土壤微生物技术在环境修复领域的深入发展与广泛应用。(图3 参44)
Abstract:
The combination of ultra-structure technology and other discipline favors the development of soils science and microbiology into more intuitive and microscopic directions. The interactions between soils and microorganisms occur all over the soil interfaces, which deeply affect the transport behavior of environmental pollutants. In this paper, the importance of micro-area analysis is carefully elucidated for the ultra-interfaces between soil and microorganisms, and the sample preparation is briefly introduced for the superstructure visualized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) coupled with energy dispersive X-ray analyses (EDX), High-Angle Annular Dark-Field (HAADF) and electron diffraction (ED) analysis. This has been further employed to observe the bacteria morphology and its intracellular organization, determine interactions of bacteria and soil components at their interfaces, determine their compositions, and study the interactions between bacteria and environmental pollutants under environmental stresses. Corresponding studies revealed that bacteria easily attached to the surface of soil particles, accelerating the biodegradation and biotransformation of pollutants by adjusting their cell surface properties. Furthermore, published research showed that the co-culture of bacteria favored the biodegradation process, which is helpful for elucidating the ultra-structure mechanisms at the micro-interfaces, tracking the trends of environmental pollutants. In a word, the application of SEM and TEM favored the development and application of bioremediation technology for contaminated soils and sediments.

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备注/Memo

备注/Memo:
收稿日期: 2017-11-15 接受日期: 2018-3-7
*国家青年自然科学基金(41501239);江苏省青年自然科学基金(BK20150976);江苏省高校自然科学基金(15KJB210003)资助
**通讯作者(E-mail: 40389@njnu.edu.cn)
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更新日期/Last Update: 2018-03-19