|本期目录/Table of Contents|

[1]张 鸽,梁开朝,辛玉华,等.基于高通量测序和传统分离研究雪茄外包皮表面细菌多样性及演替*[J].应用与环境生物学报,2018,24(04):1-10.[doi:10.3724/SP.J.1145.2017.11014]
 ZHANG Ge,LIANG Kaichao,XIN Yuhua,et al.Diversity and succession of bacteria in fermentation process of cigar wrapper by using high throughput sequencing technology and traditional isolation*[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):1-10.[doi:10.3724/SP.J.1145.2017.11014]
点击复制

基于高通量测序和传统分离研究雪茄外包皮表面细菌多样性及演替*()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
1-10
栏目:
研究论文
出版日期:
2018-08-25

文章信息/Info

Title:
Diversity and succession of bacteria in fermentation process of cigar wrapper by using high throughput sequencing technology and traditional isolation*
文章编号:
201711014
作者:
张 鸽124 梁开朝2 辛玉华2 王 娟2 李书贵14 李爱军2 王 帆3 张海波3** 刘好宝124**
1中国农业科学院烟草研究所 青岛 266101;
2中国烟草总公司海南省公司海口雪茄研究所 海口 570100 ;
3中国科学院青岛生物能源与过程研究所 青岛 266101 ;
4青岛农业大学 青岛 266109
Author(s):
ZHANG Ge124 LIANG Kaichao2 XIN Yuhua2 WANG Juan2 LI Shugui14 LI Aijun2 WANG Fan3 ZHANG Haibo3** & LIU Haobao124**
1 Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China. 2 Haikou Cigar research Institute Hainan Provincial Branch of China National Tobacco Corporation, Haikou 571100, China. 3 Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao 266101, China. 4 Qingdao Agricultural University, Qingdao 266109, China.
关键词:
雪茄外包皮高通量测序发酵时期细菌多样性群落演替
Keywords:
high throughout sequencing cigar wrapper fermentation stages bacteria diversity community succession
DOI:
10.3724/SP.J.1145.2017.11014
摘要:
细菌在烟叶发酵过程中起着重要作用,研究雪茄外包皮发酵过程中细菌群落多样性及演替对了解烟叶的发酵机理具有重要意义。本实验采用Illumina miseq高通量测序技术(High-Throughput Sequencing, HTS)和传统微生物分离方法,对墨西哥不同发酵时期雪茄外包皮表面细菌群落结构、丰富度及演替规律进行了深入研究。(1)HTS获得有效序列条数514641条,包含65个OTUs,主要微生物种属为棒状杆菌属(Corynebacterium)、葡萄球菌属(Staphylococcus)、不动杆菌属(Acinetobacter)、假单胞菌属(Pseudomonas)、芽孢杆菌属(bacillus)等。HTS结果表明,墨西哥雪茄外包皮表面细菌群落组成丰富,细菌群落结构随发酵进程而变化,优势微生物由棒状杆菌属和假单胞菌属演替后期的葡萄球菌属。α多样性结果表明FJQY-1和FJQY-2样品的细菌群落具有较高的丰富度。β多样性分析发现FJQY-2、FJQY-3和FJQY-4三个样品细菌结构及丰度较为相似。(2)传统分离结果显示,不同发酵时期雪茄外包皮表面细菌主要为芽孢杆菌属和葡萄球菌属,不能全面反映不同时期雪茄外包皮表面细菌群落结构和演替。综上所述:在不同发酵时期,雪茄外包皮表面细菌发生着群落演替;HTS测序在揭示微生物群落演替中发挥重要作用;传统微生物分离鉴定方法不能全面反映微生物多样性及群落演替,但能获得发酵过程中的部分微生物用于后期功能研究。(图4 表5 参19)
Abstract:
Objectives: Bacteria play an important role in the process of cigar fermentation. It is important to study the succession of bacteria community during the fermentation of cigar to understand the mechanism of tobacco fermentation.Methods: This experiment analysed the surface bacte rial community structure, species richness and succession of Mexican cigar wrapper of different fermentation stage using the Illumina miseq high-throughput sequencing technology (HTS) an d traditional isolation.Results: (1) HTS obtained 514641 effective sequences, including 65 OTUs. The main microbial species were Corynebacterium, Staphylococcus, Acinetobacter, Pseudomonas, Bacillus and so on. The results of HTS showed that the surface bacterial community was abundant, a nd the structure of the community changed along with the fermentation process. The dominant microorganisms from Corynebacterium and Pseudomonas to Staphylococcus in the genus level. The bacterial community of FJQY-1 and FJQY-2 had higher richness through a lpha diversity of samples . Beta diversity showed that three samples of FJQY-2, FJQY-3 and FJQY-4 had high similar . (2) The traditional isolation showed that the dominant bacteria were Bacillus and Staphylococcus during fermentation which cannot reflect the bacterial community and succession of different periods of cigar wrapper. Conclusions: All of the above, there was a succession of bacteria l community during the fermentation process. HTS plays a vital role in revealing the succession of bacterial c ommunity. Traditional isolation and identification cannot fully reflect the diver sity and succession of bacterial community, however it will be helpful later research of microbial function in the fermentation process.

相似文献/References:

[1]赵彤,蒋跃利,闫浩,等.土壤氨化过程中微生物作用研究进展[J].应用与环境生物学报,2014,20(02):315.[doi:10.3724/SP.J.1145.2014.00315]
 ZHAO Tong,JIANG Yueli,YAN Hao,et al.Research advances on microbial function in soil ammonifying process[J].Chinese Journal of Applied & Environmental Biology,2014,20(04):315.[doi:10.3724/SP.J.1145.2014.00315]
[2]许 颖** 马德胜 宋文枫 魏小芳.16S rDNA高通量测序技术分析油藏微生物多样性?[J].应用与环境生物学报,2016,22(03):409.[doi:10.3724/SP.J.1145.2015.09003]
 XU Ying**,MA Desheng,SONG Wenfeng & WEI Xiaofang.16S rDNA-assisted high-throughput sequencing analysis of microbial diversity in oil reservoirs*[J].Chinese Journal of Applied & Environmental Biology,2016,22(04):409.[doi:10.3724/SP.J.1145.2015.09003]
[3]孟会生,洪坚平,王向英,等.磷细菌肥对采煤塌陷区复垦土壤 放线菌群落的影响*[J].应用与环境生物学报,2016,22(05):911.[doi:10.3724/SP.J.1145.2016.03043]
 MENG Huisheng,HONG Jianping**,WANG Xiangying & LI Li.Effect of phosphobacteria fertilizer on soil actinomycetes community in mining subsidence area*[J].Chinese Journal of Applied & Environmental Biology,2016,22(04):911.[doi:10.3724/SP.J.1145.2016.03043]
[4]宋永亭,胡婧,吴晓玲,等.室温条件下油藏采出液微生物群落结构稳定性[J].应用与环境生物学报,2017,23(03):495.[doi:2016.07026]
 SONG Yongting,HU Jing,WU Xiaoling,et al.Stability of microbial community structure in reservoir water samples at room temperature[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):495.[doi:2016.07026]
[5]何永果,晋蕾,李果,等.基于高通量测序技术研究成年大熊猫肠道菌群[J].应用与环境生物学报,2017,23(05):771.[doi:10.3724/SP.J.1145.2016.05023]
 HE Yongguo,JIN Lei,LI Guo,et al.Gut microbiome of adult giant pandas based on high-throughput sequencing technology[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):771.[doi:10.3724/SP.J.1145.2016.05023]
[6]宋永亭.不同配气条件下微生物驱油生长代谢规律[J].应用与环境生物学报,2017,23(06):974.[doi:10.3724/SP.J.1145.2016.12036]
 SONG Yongting**.Regulation of microbial growth and metabolism and oil displacement under different gas distribution conditions[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):974.[doi:10.3724/SP.J.1145.2016.12036]
[7]徐江兵,王艳玲,罗小三,等.纳米氧化锌对堆肥过程中细菌群落演替的影响[J].应用与环境生物学报,2017,23(06):1166.[doi:10.3724/SP.J.1145.2017.04025]
 XU Jiangbing,WANG Yanling,et al.Influence of zinc oxide nanoparticles on the succession of bacterial communities during the composting process[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):1166.[doi:10.3724/SP.J.1145.2017.04025]
[8]顾磊,许科伟,汤玉平,等.基于高通量测序技术研究玉北油田上方微生物多样性[J].应用与环境生物学报,2017,23(2):276.[doi:10.3724/SP.J.1145.2016.09027]
 GU Lei,XU Kewei,et al.Microbial diversity in Yubei Oil field determined by high-throughput sequencing[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):276.[doi:10.3724/SP.J.1145.2016.09027]
[9]杨永,张学军,李寐华,等.微生物肥料对设施长期连作哈密瓜根际土壤真菌群落结构的影响[J].应用与环境生物学报,2018,24(01):68.[doi:10.19675/j.cnki.1006-687x.2017.03014]
 YANG Yong,ZHANG Xuejun,LI Meihua,et al.Effects of microbiological fertilizer on rhizosphere soil fungus communities under long-term continuous cropping of protected Hami melon[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):68.[doi:10.19675/j.cnki.1006-687x.2017.03014]
[10]任海伟,刘菲菲,李梦玉,等.玉米秸秆和白菜废弃物在不同贮存条件下的微生物群落[J].应用与环境生物学报,2018,24(02):281.[doi:10.19675/j.cnki.1006-687x.2017.05035]
 REN Haiwei,LIU Feifei,LI Mengyu,et al.Microbial community of maize straw and cabbage waste under different storage conditions[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):281.[doi:10.19675/j.cnki.1006-687x.2017.05035]

备注/Memo

备注/Memo:
收稿日期:2017-11-07 接受日期:2017-12-04
*海南省科技计划项目(ZDYF2017155);中国烟草总公司海南省公司科技重点项目(201746000024052), 中国烟草总公司海南省公司科技重点项目(201746000024056)资助
**通讯作者(E-mail: liuhaobao@caas.cn;zhanghb@qibebt.ac.cn)
点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2017-12-27