|本期目录/Table of Contents|

[1]褚翠伟 刘 斌 朱启黎 李 娜 何 健**.杀草丹降解菌Bacillus sp. T2的筛选、鉴定及降解特性*[J].应用与环境生物学报,2018,24(05):1-11.[doi:10.3724/SP.J.1145.2017.11004]
 CHU Cuiwei,LIU Bin,ZHU Qili,et al.CLC X172 Isolation, identification and degradation characteristics of a thiobencarb-degrading bacterium Bacillus sp. T2*[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1-11.[doi:10.3724/SP.J.1145.2017.11004]
点击复制

杀草丹降解菌Bacillus sp. T2的筛选、鉴定及降解特性*()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1-11
栏目:
研究论文
出版日期:
2018-10-25

文章信息/Info

Title:
CLC X172 Isolation, identification and degradation characteristics of a thiobencarb-degrading bacterium Bacillus sp. T2*
文章编号:
201711004
作者:
褚翠伟1 刘 斌2 朱启黎3 李 娜2 何 健2**
1周口师范学院生命科学与农学学院 周口 466001
2农业部农业环境微生物工程重点开放实验室 南京农业大学生命科学学院 南京 210095
3江西省南康市教师进修学校 赣州 341400
Author(s):
CHU Cuiwei1 LIU Bin2 ZHU Qili3 LI Na2 & HE Jian2**
1 College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou 466001, China?
2 Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life and Science, Nanjing Agricultural University, Nanjing 210095, China?
3 Nankang Teachers Training School, Ganzhou 341400, China
关键词:
杀草丹芽孢杆菌降解菌株筛选鉴定降解特性降解机制
Keywords:
thiobencarb Bacillus sp. T2 screening and identification of degrading-strains degradation characteristics degradation mechanism
DOI:
10.3724/SP.J.1145.2017.11004
摘要:
杀草丹(Thiobencarb)属于硫代氨基甲酸酯类除草剂,是一种重要的稻田除稗剂,其广泛长期的使用对农田生态系统造成各种危害。筛选与鉴定杀草丹高效降解菌,并研究其降解效率的影响因素及杀草丹降解代谢途径,对杀草丹污染环境的生物修复技术研发和探究杀草丹微生物降解代谢机制具有重要意义。本研究通过微生物驯化与富集技术,从长期施用杀草丹水稻田土样中分离纯化一株能够以杀草丹为唯一碳源生长的高效降解菌株T2,能够在36 h内对0.4 mmol/L(mM)的杀草丹降解率达到98.3%以上,根据其形态、生理生化特征及16S rRNA基因序列相似性分析,将其初步鉴定为芽孢杆菌属(Bacillus sp. T2)。通过GC-MS鉴定产物为:对氯苄硫醇、对氯苯甲醛和对氯苯甲酸;依据产物鉴定结果推测菌株T2通过硫酯键水解的方式起始杀草丹的降解,首先将其转化为对氯苄硫醇,随后进一步被氧化为对氯苯甲醛和对氯苯甲酸,该降解途径可能是一种新的杀草丹微生物降解代谢途径,为揭示土壤中杀草丹微生物降解代谢过程及机制提供了研究材料和理论依据。杀草丹降解菌株Bacillus sp. T2对杀草丹具有非常高的降解效率,在污染环境的微生物修复方面具有很好的应用前景。(图8 参26)
Abstract:
Thiobencarb, a thiocarbamate herbicide, is widely used to control weeds in rice paddies. Screening highly efficient thiobencarb-degrading bacteria is important for bioremediation of thiobencarb-contaminated environment. This study is aimed to isolate and identify high efficient thiobencarb-degrading bacteria and to clarify its degradation pathway and degrading properties. Thiobencarb-degrading strain was isolated by the methods of microbiological acclimation and enrichment, and then identified by the 16S rRNA gene phylogenic analysis. The degrading properties of the isolated bacterium were determined by single-factor experiments, and the degradation products were identified using gas chromatography-mass spectrometry (GC-MS). A thiobencarb-degrading strain T2, which could utilize thiobencarb as the sole carbon and energy source for growth, was isolated from the paddy soil . The strain T2 could degrade more than 98.3% of 0.4 mM thiobencarb within 36 h. It was preliminarily identified as Bacillus sp. T2 according to its 16S rRNA gene analysis, morphological, physiological and biochemical characteristics. 4-chlorobenzylmercaptan, 4-chlorobenzaldehyde and 4-chlorobenzoic acid were identified as the matebolic products of thiobencarb degradation in strain T2 by GC-MS. Based on the metabolites identification, it was speculated that tihobencarb degradation in strain T2 was initiated by hydrolysis of thioester bond to produce 4-chlorobenzyl mercaptan, which was further oxidized to 4-chlorobenzaldehyde and 4-chlorobenzoic acid. Thiobencarb degradation initiated by thioester bond hydrolysis by strain T2 is a new metabolic pathway, which supplies valuable research material and reliable experimental data to reveal metabolic process and mechanism of thiobencarb microbial degradation in soil. The strain Bacillus sp. T2 has a very high degradation efficiency, suggesting its good application prospect in microbial remediation of thiobencarb-polluted environment.

相似文献/References:

[1]王亚南,王保军,戴欣,等.近海养虾场底泥中产芽孢细菌的生态特征[J].应用与环境生物学报,2004,10(04):484.
 WANG Yanan,et al..Ecological characterization of bacilli in sediment from a nearshore marine horticultrtal region[J].Chinese Journal of Applied & Environmental Biology,2004,10(05):484.
[2]卢兰兰,李根保,沈银武,等.溶藻细菌DC-L14的分离、鉴定与溶藻特性[J].应用与环境生物学报,2009,15(01):106.[doi:10.3724/SP.J.1145.2009.00106]
 LU Lanlan,LI Genbao,SHEN Yinwu,et al.Isolation, Identification and Characterization of Blue-green Algae-lysing Strain DC-L14 from Lake Dianchi, China[J].Chinese Journal of Applied & Environmental Biology,2009,15(05):106.[doi:10.3724/SP.J.1145.2009.00106]
[3]陈谦,张新雄,赵海,等.生物有机肥中几种功能微生物的研究及应用概况[J].应用与环境生物学报,2010,16(02):294.[doi:10.3724/SP.J.1145.2010.00294]
 CHEN Qian,ZHANG Xinxiong,ZHAO Hai,et al.Advance in Research and Application of Some Functional Microbes in Bio-organic Fertilizer[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):294.[doi:10.3724/SP.J.1145.2010.00294]
[4]谢建华,师永生,杜丽琴,等.一株产酸性α-淀粉酶菌株的筛选、纯化及酶学性质[J].应用与环境生物学报,2011,17(01):95.[doi:10.3724/SP.J.1145.2011.00095]
 XIE Jianhua,SHI Yongsheng,DU Liqin,et al.Isolation and Purification of a Strain Producing Acid α-amylase and Its Enzamatic Characterization[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):95.[doi:10.3724/SP.J.1145.2011.00095]
[5]张敏文,刘悦,李荷.响应面法优化菌株Bacillus sp. W77酯酶发酵条件[J].应用与环境生物学报,2012,18(06):993.[doi:10.3724/SP.J.1145.2012.00993]
 ZHANG Minwen,LIU Yue,LI He.Optimization of Fermentation Conditions for Bacillus sp. W77 Eaterase by Response Surface Methodology[J].Chinese Journal of Applied & Environmental Biology,2012,18(05):993.[doi:10.3724/SP.J.1145.2012.00993]
[6]李珣,周存宇,田春元,等.枯草芽孢杆菌中多聚-γ-谷氨酸的生物合成及其调控机制[J].应用与环境生物学报,2013,19(04):575.[doi:10.3724/SP.J.1145.2013.00575]
 LI Xun,ZHOU Cunyu,TIAN Chunyuan,et al.Biosynthesis of Poly-γ-glutamic Acids and Relevant Regulatory Networks in Bacillus subtilis[J].Chinese Journal of Applied & Environmental Biology,2013,19(05):575.[doi:10.3724/SP.J.1145.2013.00575]
[7]张新雄,彭锋,毛光平,等.饲用芽孢杆菌研究与应用进展[J].应用与环境生物学报,2013,19(05):891.[doi:10.3724/SP.J.1145.2013.00891]
 ZHANG Xinxiong,PENG Feng,MAO Guangping,et al.Advance in Research and Application of Bacillus Probiotics[J].Chinese Journal of Applied & Environmental Biology,2013,19(05):891.[doi:10.3724/SP.J.1145.2013.00891]
[8]李雷,冯红.两株芽孢杆菌降解羽毛比较及抗氧化肽分离[J].应用与环境生物学报,2018,24(01):172.[doi:10.19675/j.cnki.1006-687x.2017.02006]
 LI Lei,FENG Hong**.Comparison of feather degradation by two $Bacillus$ strains and separation of antioxidant peptides*[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):172.[doi:10.19675/j.cnki.1006-687x.2017.02006]
[9]褚翠伟,刘斌,朱启黎,等.杀草丹降解菌Bacillus sp. T2的筛选、鉴定及降解特性[J].应用与环境生物学报,2018,24(04):921.[doi:10.19675/j.cnki.1006-687x.2017.11004]
 CHU Cuiwei,LIU Bin,ZHU Qili,et al.Isolation, identification, and the degradation characteristics of a thiobencarb-degrading bacterium Bacillus sp. T2[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):921.[doi:10.19675/j.cnki.1006-687x.2017.11004]

备注/Memo

备注/Memo:
收稿日期:2017-11-03 接受日期:2017-12-04
*国家重点研发计划子课题(2016YFD0801102)、江苏省科技厅科技攻关项目(BE2016374)资助
**通讯作者(E-mail: hejian@njau.edu.cn)
点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-01-10