|本期目录/Table of Contents|

[1]罗佳慧 王 虹** 陈 玲.地铁空气微生物研究进展*[J].应用与环境生物学报,2018,24(04):1-10.[doi:10.3724/SP.J.1145.2017.10007]
 LUO Jiahui,WANG Hong** & CHEN Ling.Advances in research of airborne microbes in subway systems*[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):1-10.[doi:10.3724/SP.J.1145.2017.10007]
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地铁空气微生物研究进展*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
1-10
栏目:
综述
出版日期:
2018-08-25

文章信息/Info

Title:
Advances in research of airborne microbes in subway systems*
文章编号:
201710007
作者:
罗佳慧 王 虹** 陈 玲
同济大学环境科学与工程学院, 污染控制与资源化研究国家重点实验室 上海 200092
Author(s):
LUO Jiahui WANG Hong** & CHEN Ling
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
关键词:
地铁空气微生物主动采样分子生物学分布特征影响因素
Keywords:
subway airborne microbes active sampling molecular biology distribution characteristics influence factors
DOI:
10.3724/SP.J.1145.2017.10007
摘要:
地铁已发展成为现代城市中最重要的交通工具之一,由于地铁环境的特殊性(相对封闭、人流量大等),空气较易受微生物污染,对公众健康构成潜在威胁,近年来受到了各国研究者的关注。本文通过比较多种空气微生物采样和分析方法,发现主动采样和基于聚合酶链式反应(Polymerase chain reaction,PCR)的分子生物学分析方法如16S rRNA基因测序、宏基因组学及定量PCR技术可为地铁空气微生物的污染特征提供更为准确、全面和细致的信息;通过对全球范围内不同地区地铁空气微生物文献的分析,发现地铁空气微生物的分布特征随时间和空间可发生较大变化,影响地铁内空气中细菌和真菌的浓度及种类的主要因素包括环境温度、相对湿度、二氧化碳浓度、人流量、屏蔽门、通风系统等。建议未来尽快建立基于分子生物学分析的标准化采样和分析方法,进而获得地铁空气微生物污染水平的基线数据,并建立空气中致病菌浓度与人体健康风险的相关性。(图1 表3 参56)
Abstract:
Subway systems are indispensable for transportation in modern cities, of which the air is easily polluted by microbes due to the enclosed and crowded environment. Microbiological characteristics of subway aerosols have attracted researchers’ attention worldwide in recent years. This review aimed to evaluate the sampling and analyzing methods suitable for detecting airborne microbes in subway systems. Active air sampling methods and molecular biology techniques based on polymerase chain reaction such as 16S rRNA gene sequencing, metagenomics and quantitative PCR demonstrated great potential in microbial analysis of aerosols given their capabilities of providing accurate and comprehensive information. Temporal and spatial distributions of airborne microbes, as well as the factors influencing their distribution characteristics were also discussed by critically reviewing worldwide literatures on subway microbiome studies. The concentration s and composition s of airborne bacteria and fungi in subway can vary dramatically with time and space. The critical factors determining microbial community structures and compositions include temperature, relative humidity, carbon dioxide concentration, number of passengers, presence of platform screen door, ventilation systems, etc. The review suggests that future studies on standardized sampling and analyzing protocols should be needed to obtain contamination levels of subway airborne microbes and transform microbial monitoring data to public health risks.

相似文献/References:

[1]陈皓文.南极菲尔德斯半岛空气微生物含量初报[J].应用与环境生物学报,1997,3(02):180.
 Chen Haowen.OUTDOOR AIR-BORNE MICROBIAL COUNTS OVER THE FILDES PENINSULA, ANTARCTICA[J].Chinese Journal of Applied & Environmental Biology,1997,3(04):180.

备注/Memo

备注/Memo:
收稿日期:2017-10-11 接受日期:2017-12-18
*中央高校基本科研业务费专项资金资助
**通讯作者(E-mail: hongwang@tongji.edu.cn)
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更新日期/Last Update: 2018-01-10