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[1]王海斌,** 陈晓婷 丁力 邱丰艳 叶江华 贾小丽 孔祥海.不同树龄茶树根际土壤细菌多样性的T-RFLP分析[J].应用与环境生物学报,2018,24(04):1-11.[doi:10.3724/SP.J.1145.2017.10003]
 WANG Haibin,**,CHEN Xiaoting,et al.Using T-RFLP technology to analyze bacteria diversity in rhizospheric soil of different age tea tree*[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):1-11.[doi:10.3724/SP.J.1145.2017.10003]
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不同树龄茶树根际土壤细菌多样性的T-RFLP分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
1-11
栏目:
研究论文
出版日期:
2018-08-25

文章信息/Info

Title:
Using T-RFLP technology to analyze bacteria diversity in rhizospheric soil of different age tea tree*
文章编号:
201710003
作者:
王海斌1 2** 陈晓婷1 丁力1 邱丰艳1 叶江华3 贾小丽3 孔祥海1
1龙岩学院生命科学学院 龙岩 364012
2福建农林大学福建省农业生态过程与安全监控重点实验室 福州 350002
3武夷学院 武夷山 354300
Author(s):
WANG Haibin1 2** CHEN Xiaoting1 Ding Li1 Qiu Fengyan1 YE Jianghua3 JIA Xiaoli3 & KONG Xianghai1
1College of Life Sciences, Longyan University, Longyan 364012, China2Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou 350002, China 3Wuyi University, Wuyishan 354300, China
关键词:
茶树不同树龄根际土壤细菌多样性T-RFLP
Keywords:
tea tree with different ages rhizospheric soil bacteria diversity T-RFLP
DOI:
10.3724/SP.J.1145.2017.10003
摘要:
为了解不同树龄茶树根际土壤细菌多样性变化, 以不同树龄铁观音茶树根际土壤为研究对象,采用末端限制性片段长度多样性(T-RFLP)技术分析不同树龄茶树对土壤细菌多样性的的影响。结果显示,茶树根际土壤细菌HaeIII和MspI酶切产物的T-RFs片段数量及辛普森指数、香农威纳指数均随着茶树树龄的增加呈现下降趋势。相关性分析结果表明,与茶树树龄呈显著或极显著正相关的细菌T-RFs片段9个,共34种细菌由10个纲组成,按照功能可分成,病原菌及与改善土壤质地、抑制病原菌、碳素循环、氮素循环、硫素循环等相关的微生物,6大类,其中病原菌占比达58.82%。与茶树树龄呈显著或极显著负相关的细菌T-RFs片段17个,共38种细菌由12个纲组成,按照功能可分成,病原菌及与氮素循环、碳素循环、抑制病原菌、改善土壤质地等相关的细菌,共5大类,其中除病原菌外,其余细菌占比达78.95%。综上表明,随着茶树树龄的增加,茶树根际土壤细菌多样性及其功能发生了显著的变化,为后期细菌群落互作效应研究的提供了一定理论基础。(图4 表3 参81)
Abstract:
To explore the change of bacteria diversity in rhizospheric soil, rhizospheric soils of Tieguanyin with different ages were used as materials, and the terminal-restriction fragment length polymorphism (T-RFLP) technology was used to analyze bacteria diversity. The results showed that the T-RFs numbers, the simpson index, and shannon index were all significantly decreased as the age of tea tree increased. Correlation analysis result showed that 9 T-RFs from bacterial community were significantly and positively correlated with the tea tree age, which included 34 species of microbes belonging to 10 classes, respectively. The thirty-four microbes can be divided into 6 types according to the function, such as pathogenic bacteria, bacteria to improve the soil texture, bacteria to inhibit pathogenic bacteria, carbon cycle, nitrogen cycle and sulphur cycle. Furthermore, the percentage of pathogenic bacteria reached 58.82% in that. In addition, 17 T-RFs were significantly and negatively correlated with the tea tree age, which included 38 species of bacteria belonging to 12 classes, respectively. The thirty-eight bacteria can be divided into 5 types according to the function, such as pathogenic bacteria, bacteria to improve the soil quality, bacteria to inhibit pathogenic bacteria, carbon cycle, and nitrogen cycle. Furthermore, others except the pathogenic bacteria accounted for 78.95%. In brief, bacteria diversity and function in rhizospheric soil of tea tree changed significantly as the age of tea tree increased, these results provided the theoretical basis for studying on the interaction of bacterial communities.

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备注/Memo

备注/Memo:
收稿日期:2017-10-09 接受日期:2017-11-03
*国家重点研发计划(2016YFD0200900)、国家948项目(2014-Z36)、中国博士后科学基金(2016M600493)、福建省自然科学基金青年基金(2017J05057)、福建省中青年教师教育科研项目(JAT170573)、龙岩学院人才引进项目(LB2015001) 资助
**通讯作者(E-mail: w13599084845@sina.com)
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更新日期/Last Update: 2017-12-07