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[1]徐小萍 陈晓慧 吕科良 陈旭 陈裕坤 林玉玲 赖钟雄**.龙眼漆酶家族成员全基因组结构与功能分析*[J].应用与环境生物学报,2018,24(04):1-16.[doi:10.3724/SP.J.1145.2017.09047]
 XU Xiaoping,CHEN Xiaohui,Lv Keliang,et al.Genome-wide Identification and Function analysis of the Laccase gene family in Dimocarpus longan Lour.*[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):1-16.[doi:10.3724/SP.J.1145.2017.09047]
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龙眼漆酶家族成员全基因组结构与功能分析*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
1-16
栏目:
研究论文
出版日期:
2018-08-25

文章信息/Info

Title:
Genome-wide Identification and Function analysis of the Laccase gene family in Dimocarpus longan Lour.*
文章编号:
201709047
作者:
徐小萍1 陈晓慧1 吕科良1 陈旭1 陈裕坤1 林玉玲1 赖钟雄1**
1福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
XU Xiaoping1CHEN Xiaohui1 Lv Keliang1 CHEN Xu1 CHEN Yukun1 LIN Yuling1& LAI Zhongxiong1**
1Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
龙眼漆酶家族成员鉴定功能分析miRNA预测
Keywords:
Dimocarpus longan Lour. laccase family gene identification functional analysis miRNA prediction
DOI:
10.3724/SP.J.1145.2017.09047
摘要:
为了解龙眼生物信息学分析方法龙眼基因组LAC基因启动子、进化。结果表明:,包含2个以上糖基化位点,具有多个保守的motif;5端调控序列预测分析,DlLAC可能具有多个转录起始位点;DlLACp启动子序列均包含大量非生物胁迫应激响应元件、胚乳特异表达元件及MYB结合位点,推测MYB可能通过DlLAC进而调控龙眼胚胎发育。DlLAC9-1p、DlLAC12p、DlLAC17-2p含黄酮类化合物合成调控的MYB结合位点, 可能参与龙眼生长发育过程中种子成熟和果皮成色的色素合成途径;DlLAC家族成员可能参与不同体胚发育过程和不同组织器官形态建成。42个龙眼漆酶成员共有28个受miRNA调控,13个成员受miR397调控,可能与木质素合成相关,8个成员受miR1535调控。非miR397调控的成员可能发挥其他重要的生物学功能。分析表明,DlLAC家族成员在龙眼生长发育过程中除了参与木质素合成以外,还可能参与胚胎发育、胚乳发育、色素合成,组织器官特异表达等重要生物学功能,表明其功能上的多样性。(图9表2参38)
Abstract:
Objectives: Investigating the biological function of the Laccase (DlLAC) gene family in Dimocarpus longan Lour.Methods: The DlLAC gene family members were identified, the gene structure, protein domain, transcription start site, cis-acting element of promoter, phylogenetic trees, FPKM analysis of somatic,embryogenic cultures at differentdevelopmental stages and different tissues or organs of longan and possibly interacting miRNAs in the longan genome were predicted by bioinformatic analysis. The possible biological function of DlLAC were analyzed in longan.Results: In total, 42 members of the DlLAC gene family were identified into seven groups. The gene structure analysis indicated that the number of introns of DlLAC gene family ranged from 1 to 8, mainly in 5, only DlLAC15-1 containing 600 bp short tandem repeats. The conserved domains of proteins were divided into 5 types, all of which belonged to covelline albumen family, containing 1~5 protein domains, respectively, with three copper blue oxidase domains predominantly. DlLAC proteins belonged to the secretory pathway, subcellular localization in the out of cell, contained more than two glycosylation sites and multiple conserved motifs. The transcription start site and cis-acting elements were predicted that the laccase family might have multiple transcription start sites, the cis-acting elements of promoter contained a large number of abiotic stress response elements, endosperm-specific expression response elements and MYB binding sites, which suggest that MYB should probably regulate the development of longan embryos through DlLAC. While DlLAC9-1p, DlLAC12p and DlLAC17-2p contained MYB binding sites regulating the synthesis of flavonoid compounds, which suggested that the process of seed ripening and fruit color formation during the process of longan growth and development might be influenced. Members of the DlLAC family may be involved in the development of somatic,embryos and the morphogenesis of different tissues and organs. A total of 30 longan laccase sequences were likely regulated by miRNAs, 13 of which were mainly regulated by miR397, which might be associated with lignin synthesis, rather than members of the miR397 regulation might play other important biological functions.Conclusions: DlLAC gene family members might be involved in lignin biosynthesis,as well as the development of the somatic,embryos, different tissues, endosperm development, pigment synthesis,and other important biological functions during the growth and development in longan, which showed their diverse functions.

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备注/Memo

备注/Memo:
收稿日期:2017-09-30 接受日期:2017-11-22
*国家自然科学基金(31572088,31672127);福建省科技重大专项(2015NZ0002-1)资助
**通讯作者(E-mail:Laizx01@163.com)
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更新日期/Last Update: 2017-12-07