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[1]徐小娜 崔浩天 陈亮宇 苏春 白凤武 赵心清**.具有抗补体活性海洋放线菌的筛选及其活性物质的分离*[J].应用与环境生物学报,2018,24(05):1-9.[doi:10.3724/SP.J.1145.2017.09038]
 XU Xiaona,CUI Haotian,CHEN Liangyu,et al.Screening of anti-complementary agent producing marine actinomycetes and isolation of active fractions *[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1-9.[doi:10.3724/SP.J.1145.2017.09038]
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具有抗补体活性海洋放线菌的筛选及其活性物质的分离*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1-9
栏目:
研究论文
出版日期:
2018-10-25

文章信息/Info

Title:
Screening of anti-complementary agent producing marine actinomycetes and isolation of active fractions *
文章编号:
201709038
作者:
徐小娜1 崔浩天2 陈亮宇1 苏春3 白凤武2 赵心清2**
1大连理工大学生命科学与技术学院 大连 116023?
2上海交通大学生命科学技术学院微生物代谢国家重点实验室 上海 200240?
3 陕西师范大学生命科学学院 陕西 西安710119
Author(s):
XU Xiaona1 CUI Haotian2 CHEN Liangyu1 SU Chun3 BAI Fengwu2 & ZHAO Xinqing2**
1School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China 2State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China 3College of Life Sciences, Shaanxi Normal University, Xian 710119, China
关键词:
海洋放线菌抗补体活性次生代谢产物分离纯化
Keywords:
marine actinomycete anti-complement activity secondary metabolites isolation and purification
DOI:
10.3724/SP.J.1145.2017.09038
摘要:
抗补体活性物质的研究对开发相关药物,治疗多种补体过度或非正常激活引起的疾病具有重要意义。然而,目前对微生物来源的抗补体活性物质的研究还非常有限。本文利用溶血法对从大连星海湾海泥样品中分离出来的42株海洋放线菌进行了抗补体活性测试,并利用C18柱色谱层析及Sephadex LH-20 凝胶柱层析,对具有较好抗补体活性的菌株DUT11的次生代谢产物进行了分离纯化和结构鉴定。结果显示:菌株 S187 、M5、S088、M8、S063、DUT11 和 MD16 在TSB 培养基中发酵后发酵液提取物和菌丝体提取物均具有较好的抗补体活性,其中菌株 DUT11 的抗补体活性为最强。DUT11在M33培养基中发酵后所产生的次生代谢产物的抗补体活性最强,其发酵液和菌丝体提取物的活性分别为56.5% 和 60.8% 。进一步分离得到了3个具有抗补体活性的小分子化合物,分别鉴定为3-吲哚甲酸(1)、对羟基苯甲酸(2)和2-吡咯甲酸(3)。本研究表明,在大连星海湾海泥样品中存在多种可产生抗补体活性物质的海洋链霉菌,其菌种丰富多样,产生的抗补体活性化合物活性较强,这些都为进一步开发利用海洋放线菌提供了新的思路,为研究新型抗补体活性化合物奠定了基础。(图3 表3 参26)
Abstract:
Studies on anti-complement agents are essential for development of drugs to treat various diseases aroused by excessive or abnormal activation of complementary system. However, results on microbial-derived anti-complement agents are still very limited. The anti-complement activities of 42 marine-derived actinomycete strains isolated from the sediment samples from Dalian Xinghai Bay were studied using the hemolysis method. In addition, the active fraction s of the strain DUT11 was separated by C18 and Sephadex LH-20 column chromatography, and the compounds with anti-complement activity were identified . Strains S187, M5, S088, M8, S063, DUT11 and MD16 showed better anti-complement activities when being cultured in TSB medium, and DUT11 showed the highest anti-complement activity. Strain DUT11 presented the highest anti-complement activity in M33 fermentation medium. The anti-complement activity was 56.5% for the extracts of supernatant and 60.8% for the extracts of mycelia. Furthermore, three compounds with anti-complement activity were obtained, which are 3-indolecarboxylic acid (1), p-hydroxy benzoic acid (2) and 2-pyrrolecarboxylic acid (3). Marine-derived streptomycetes can be employed to produce active compounds with the anti-complement activity. This study presents a new alternative for utilization of marine actinomycetes, and provides basis for exploration of new anti-complement agents.

备注/Memo

备注/Memo:
收稿日期: 2017-09-26 接受日期: 2017-12-19
*中国科学院热带海洋生物资源与生态重点实验室开放课题项目(LMB111002)、国家自然科学基金青年项目(31600038)和中央高校基本科研业务费专项资金项目(GK201703035)资助?
**通讯作者(E-mail:xqzhao@sjtu.edu.cn)
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更新日期/Last Update: 2018-02-07