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[1]王彦伟 阮志勇 江旭 张君陶 吴波 秦晗 谭芙蓉 胡国全何明雄 **.一株耐受糠醛的纤维素降解菌Bacillus sp. BREC-11的分离与鉴定[J].应用与环境生物学报,2018,24(04):1-8.[doi:10.3724/SP.J.1145.2017.09006]
 WANG Yanwei,RUAN Zhiyong,JIANG Xu,et al.Screening and Identification of cellulose degrading bacterium Bacillus sp. BREC-11 with tolerance to furfural[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):1-8.[doi:10.3724/SP.J.1145.2017.09006]
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一株耐受糠醛的纤维素降解菌Bacillus sp. BREC-11的分离与鉴定()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
1-8
栏目:
研究论文
出版日期:
2018-08-25

文章信息/Info

Title:
Screening and Identification of cellulose degrading bacterium Bacillus sp. BREC-11 with tolerance to furfural
文章编号:
201709006
作者:
王彦伟12 阮志勇2 江旭2 张君陶3 吴波1 秦晗1 谭芙蓉1 胡国全1何明雄1 **
1.农业部沼气科学研究所 成都 640001?
2.农业部农业微生物资源收集与保藏重点实验室 北京 100081?
3.四川师范大学 成都 610101
Author(s):
WANG Yanwei123RUAN Zhiyong2JIANG Xu2 ZHANG Juntao3WU Bo1QIN Han1TAN Furong1HU Guoquan1&HE Mingxiong1**
1. Biogas Institute of Ministry of Agriculture, Chengdu 610041, China?
2. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of AgricultureBeijing 100081, China?
3. Sichuan Normal University, Chengdu 610101 , China
关键词:
竹虫Bacillus sp .糠醛纤维素降解
Keywords:
Omphisa fuscidentalis Hampson Bacillus sp furfural cellulose-degrading
DOI:
10.3724/SP.J.1145.2017.09006
摘要:
以糠醛为耐受物添加到培养基中,竹虫幼虫肠道作为分离源,经刚果红染色法初步筛选,分离到一株可耐受糠醛的纤维素降解菌株BREC-11, 通过形态学观察,生理生化分析,细胞化学分析,16Sr DNA序列比对等多相分类学方法鉴定,确定其属于芽孢杆菌属的一个种,将其定名为Bacillus sp. BREC-11. 不同浓度糠醛耐受试验研究发现,菌株BREC-11在含3.5g/L糠醛的培养基中可以生长,在3.5g/L糠醛的耐受浓度下,测定菌株BREC-11的滤纸酶活(FPA),CMC酶活(CMC),纤维二糖酶活(β-G),结果表明,菌株在30℃,150rmp培养2d后,滤纸酶活达到0.1 U/ml,CMC酶活达到0.21 U/ml,纤维二糖酶活达到0.07U/ml。 研究表明BREC-11是一株耐受糠醛的纤维素降解菌株,在生物炼制过程中具有一定的应用潜力。
Abstract:
Objectives: A cellulose-degrading bacterium BREC-11 with to lerance to furfural was isolated from the intestinal tract of the larvae of Omphisa fuscidentalis Hampson via the addition of furfural in medium. Methods: Based on the analyses of morphological obvservation, the physiological and biochemical characterization,and the 16S rDNA sequences, strain BREC-11 was suggested to represent a member of the genus Bacillus sp, which named Bacillus sp. BREC-11. Results: To study the tolerating concentration of strain BREC-11, wide range of furfural formaldehyde concentration was tested and showed that strain BREC-11 was able to grow in the mineral medium containing furfural up to 3.5 (g / L).The cellulase activity of the strain BREC-11 was determined at the tolerable concentration of 3.5 (g / L) fur fural after incubation at 30 ℃, 150 rpm for 2 days . The results indicated that filter paper enzyme, CMC-Na enzyme and β-glucosidase activity was 0.1 U / ml, 0.21 U / ml and 0.07U / ml ,respectively. Conclusions: BREC-11 is a cellulose-degrading bacterium with resistantance to furfural , which has certain potential application in the process of biorefinery in the future.

备注/Memo

备注/Memo:
收稿日期:2017-09-05 接受日期: 2017-09-30
*国家自然科学基金项目(31600012),农业部农业微生物资源收集与保藏重点实验室开放基金项目(2015003),农业部沼气所基本科研业务费专项项目(12016206030201710和12017206030202211)资助
**通讯作者(E-mail:hemingxiong@caas.cn)
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更新日期/Last Update: 2017-11-28