|本期目录/Table of Contents|

[1]林心宇 张颖 张伟妮 吴方达 黄一帆 黄小红 **.镉离子对尼罗罗非鱼精巢N-乙酰-β-D-氨基葡萄糖苷酶的失活动力学研究[J].应用与环境生物学报,2018,24(04):1-13.[doi:10.3724/SP.J.1145.2017.08027]
 LIN Xinyu,ZHANG Ying,ZHANG Weini,et al.Inhibitory kinetics of β-N-acetyl-D-glucosaminidase from Nile tilapia (Oreochromis niloticus) by cadmium[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):1-13.[doi:10.3724/SP.J.1145.2017.08027]
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镉离子对尼罗罗非鱼精巢N-乙酰-β-D-氨基葡萄糖苷酶的失活动力学研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
1-13
栏目:
研究论文
出版日期:
2018-08-25

文章信息/Info

Title:
Inhibitory kinetics of β-N-acetyl-D-glucosaminidase from Nile tilapia (Oreochromis niloticus) by cadmium
文章编号:
201708027
作者:
林心宇 1 张颖 1 张伟妮 1 吴方达 2 黄一帆 1 黄小红 **1
1福建省兽医中药与动物保健重点实验室(福建农林大学) 福州 3500022宁德市动物疾病预防控制中心 宁德 352100
Author(s):
LIN Xinyu1 ZHANG Ying1 ZHANG Weini1 WU Fangda2 HUANG Yifang 1&HUANG Xiaohong**1
1Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou 350002, China2Ningde centers for Animal Disease Control and Prevention, Ningde 352100, China
关键词:
抑制动力学N-乙酰-β-D-氨基葡萄糖苷酶罗非鱼镉离子
Keywords:
Inhibitory kinetics β-N-acetyl-D-glucosaminidase Nile tilapia Cd2+
DOI:
10.3724/SP.J.1145.2017.08027
摘要:
镉离子污染对水生生物的繁殖造成了不利影响,N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)被认为参与了动物的受精过程,因此,本研究以尼罗罗非鱼精巢分离获得的NAGase酶为研究对象,研究镉离子对其活力的影响,采用底物反应动力学方法,建立镉离子对NAGase酶失活模型,探究镉离子对该酶活性的影响。结果显示,镉离子对NAGase表现出了可逆的抑制,其对NAGase抑制的IC50为40.95 mM。镉离子对NAGase的抑制机理为竞争型抑制,抑制常数KI为17.13 mM,同时测定了微观失活速度常数。本研究结果可为后期镉离子对罗非鱼繁殖的研究提供理论依据。(图8 表1 参24)
Abstract:
Cadmium pollution in the aquatic environment can negatively affect some reproductive parameters to aquatic animals. Meanwhile, the β-N-acetyl-D-glucosaminidase (NAGase) is thought to play an important role in the fertilization process. Herein, This paper aimed to study the effect of Cd2+ on the activity of NAGase purified from the testis of Nile tilapia. The kinetic method of substrate reaction was used, and an inhibitory model was established to study the kinetics of NAGase under inhibition by Cd2+. The results showed that Cd2+ could reversibly inhibit the enzymatic activity of NAGase, and the IC50 was estimated to be 40.95 mM. Cd2+ was found to be a competitive inhibitor of NAGase. The inhibitory constant was determined to be 17.13 mM. The microscopic rate constants of inactivation were also determined. These results may provide some theoretical foundation for the further studies on reproduction of tilapia.

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[3]张伟妮,陈晓佳,沈林松,等.强氯精对尼罗罗非鱼精巢N-乙酰-β-D-氨基葡萄糖苷酶的失活动力学[英文][J].应用与环境生物学报,2017,23(2):306.[doi:10.3724/SP.J.1145.2016.04033]
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[4]沈林松,张 颖,张伟妮,等.果糖对莆田黑猪精液中NAGase的抑制动力学[J].应用与环境生物学报,2015,21(01):46.[doi:10.3724/SP.J.1145.2014.07013]
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备注/Memo

备注/Memo:
收稿日期:2017-08-21 接受日期:2017-09-19
*福建省自然科学基金项目(2012N0002)资助
**通讯作者(E-mail:xhhuang138@hotmail.com)
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更新日期/Last Update: 2017-12-26