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[1]卢倩云 曹宇棽 陈友明 晏 琼**.镉胁迫下油菜毛状根的生理响应及铁钾含量*[J].应用与环境生物学报,2018,24(05):1-12.[doi:10.19675/j.cnki.1006-687x.2017.06019]
 LU Qianyun,CAO Yushen,CHEN Youming & YAN Qiong**.The physiological response and Fe, K contents in hairy roots of Brassica rape L. under Cd stress*[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1-12.[doi:10.19675/j.cnki.1006-687x.2017.06019]
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镉胁迫下油菜毛状根的生理响应及铁钾含量*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1-12
栏目:
研究论文
出版日期:
2018-10-25

文章信息/Info

Title:
The physiological response and Fe, K contents in hairy roots of Brassica rape L. under Cd stress*
文章编号:
201706019
作者:
卢倩云 曹宇棽 陈友明 晏 琼**
北京交通大学理学院生命科学与生物工程研究院 北京 100044
Author(s):
LU Qianyun CAO Yushen CHEN Youming & YAN Qiong**
College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China
关键词:
油菜毛状根抗氧化酶活性氧化损伤铁钾元素含量
Keywords:
cadmium brassica rape hairy roots antioxidant enzymes activity oxidative damage iron and potassium accumulation amounts
DOI:
10.19675/j.cnki.1006-687x.2017.06019
摘要:
利用液体悬浮培养法研究不同镉浓度(0, 25, 50, 100, 200, 400 μmol/L)胁迫下油菜毛状根的生理响应及对铁钾含量的影响,以探究油菜毛状根对镉胁迫的耐受与富集能力。研究结果表明:1)低镉浓度(100 μmol/L以下)对毛状根的生长无显著影响,高镉浓度(100 μmol/L以上)下毛状根的生长则受到明显的抑制,25 μmol/L镉胁迫7 d 时毛状根的鲜重最大(4.34 g)。 2 )油菜毛状根中活性氧(ROS)的含量随着镉浓度的增加而上升,根中主要抗氧化酶(SOD、POD、CAT)的活性在镉胁迫1 d时,表现出先降低后升高的趋势;镉胁迫7 d时,表现出先升高后降低的趋势。3)PI染色与MDA分析表明,根细胞的损伤随着镉浓度的增加越发严重。4)油菜毛状根中的镉含量随着培养基中镉浓度的增加而增加,400 μmol/L、7 d时,达到最大值2.97 mg/ g。毛状根中的铁含量在镉胁迫1 d时随着镉浓度的增加显著上升,最大值达到14.52 mg/g;镉胁迫7 d时没有明显变化。镉胁迫7 d时毛状根中的钾含量是镉胁迫 1 d 时(15.73 mg/g)的 1.6 倍。研究结果表明,油菜毛状根对镉胁迫的生理响应变化与镉的作用浓度和时间相关;同时镉胁迫造成了毛状根中铁、钾元素代谢紊乱,但油菜毛状根对镉有较好的富集效果。(图8 参30)
Abstract:
The experiments were conducted to explore the tolerance and enrichment capabilities by researching the physiological response and the cadmium impact on iron (Fe) & potassium (K) accumulation amounts of brassica rape hairy roots under different cadmium concentrations using liquid suspension culture method. The results show that: 1) The growth of hairy roots have no significant difference under low cadmium concentrations (below 100 ? mol/L) stress, while obviously inhibited under high cadmium concentrations (more than 100 ?mol/L) stress. And the fresh weight of brassica rape hairy roots reaches maximum of 4.34 g after 7 d’s 25 ?mol/L cadmium stress; 2) The content of ROS in brassica rape hairy roots increase with the increasing concentrations of cadmium; the antioxidant enzymes activities of brassica rape hairy roots (SOD, POD, CAT) decrease first and then increase with the increasing cadmium concentrations under 1 d’s stress, while under 7 d’s stress the trend is the opposite. 3) PI staining and the content of MDA indicate that the cellular damage is more serious along with the increase of the cadmium concentrations; 4) With the increase of cadmium concentrations, cadmium content of hairy roots increases, and reach maximum of 2.97 mg/g after 7 d’s 400 ? mol/L cadmium stress. The iron content also increases significantly with the maximal weight of 14.52 mg/g after 1 d’s cadmium stress, while there is no significant difference under 7 d’s cadmium stress. The potassium content under 7 d’s cadmium stress is 1.6 times of the 1 d’s stress ( 15.73 mg/g). The study shows that the physiological response of brassica rape hairy roots was correlated with the concentration and time of cadmium stress. At the same time, cadmium stress caused the metabolic disorders of iron and potassium in the hairy roots, but the hairy roots of brassica rape have better enrichment effect on cadmium.

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备注/Memo

备注/Memo:
收稿日期: 2018-03-01 接受日期: 2018-04-16
*中央高校基本科研业务费(2014JBM118)资助
**通讯作者(E-mail: qyan@bjtu.edu.cn)
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更新日期/Last Update: 2018-06-05