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[1]李芙蓉,丁涛,白林含.拟茎点霉属Phomopsis sp. S4菌株发酵产物对稻瘟病菌细胞膜的抑制作用[J].应用与环境生物学报,2018,24(02):342-346.[doi:10.19675/j.cnki.1006-687x.2017.04013]
 LI Furong,DING Tao,BAI Linhan.Inhibition mechanism of fermentation broth extract of Phomopsis sp. strain S4 on cell membranes of Magnaporthe oryzae[J].Chinese Journal of Applied & Environmental Biology,2018,24(02):342-346.[doi:10.19675/j.cnki.1006-687x.2017.04013]
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拟茎点霉属Phomopsis sp. S4菌株发酵产物对稻瘟病菌细胞膜的抑制作用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年02期
页码:
342-346
栏目:
研究论文
出版日期:
2018-04-25

文章信息/Info

Title:
Inhibition mechanism of fermentation broth extract of Phomopsis sp. strain S4 on cell membranes of Magnaporthe oryzae
作者:
李芙蓉丁涛白林含
四川大学生命科学学院,生物资源与生态环境教育部重点实验室 成都 610065
Author(s):
LI Furong DING Tao BAI Linhan
Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
关键词:
内生真菌拟茎点霉属稻瘟病抑菌活性细胞膜麦角甾醇
Keywords:
endophytic fungus Phomopsis Magnaporthe oryzae antifungal effect cell membrane ergosterol
分类号:
Q939.95
DOI:
10.19675/j.cnki.1006-687x.2017.04013
摘要:
内生真菌拟茎点霉属真菌Phomosis sp. S4菌株发酵次生代谢产物对多种植物病原菌具有良好的抑菌效果,在防治农作物病害上具有应用前景. 为探究S4菌株发酵产物对植物病原真菌的抑制机理,以稻瘟病菌为研究材料,通过普通抑菌实验、扫描电镜、荧光定量PCR以及生理实验,检测S4菌株发酵液提取物对稻瘟病菌细胞膜的作用情况. 扫描电镜实验显示经S4菌株提取物处理的稻瘟病菌菌丝严重变形,菌丝表面出现大量褶皱,表明提取物对稻瘟病菌细胞膜和细胞壁的完整性造成破坏. 荧光定量PCR检测到细胞膜中重要组成成分麦角甾醇合成途径中相关基因的变化情况,麦角甾醇合成途径中ERG1、ERG11、ERG6基因分别下调了2.0、1.40、2.7倍,ERG7基因上调了1.38倍. 随后的生理实验也表明经过S4提取物处理的稻瘟病菌确实存在细胞内容物泄露的情况. 本研究表明S4提取物通过抑制麦角甾醇合成而破坏细胞膜的完整性导致细胞内容物泄露,从而达到抑制真菌生长效果. (图7 表1 参16)
Abstract:
The secondary metabolites of endophytic Phomopsis sp. strain S4 show antifungal activity against a variety of plant pathogens, which implies that strain S4 has potential prospect in crop disease control. The aim of this study was to explore the inhibition mechanisms of S4 against plant pathogenic fungi. Magnaporthe oryzae was used as the main pathogenic material for the research. Cell membrane changes were detected using antifungal experiments, scanning electron microscopy, Q RT-PCR, and cell content leaking experiments. The results of scanning electron microscopy showed that M. oryzae mycelia, after treatment with S4 fermented product extract, decreased in size, suggesting the integrity of the cell membrane was destroyed. The genes related to ergosterol synthesis, which plays an important role in membrane integrity, were studied though Q RT-PCR. The results showed that the expression levels of ERG1, ERG11, and ERG6 genes were down-regulated by 2.0, 1.40, and 2.7-fold, respectively, whereas that of ERG7 was up-regulated by 1.38-fold, which means the ergosterol synthesis pathway was destroyed. The physiological experiments also showed that the cell contents of M. oryzae mycelia treated with S4 fermented product extract leaked significantly, which was consistent with the Q RT-PCR results. The results showed that S4 fermentation broth exact could destroy the cell membrane integrity by inhibiting ergosterol synthesis, and eventually inhibit M. oryzae cell growth.

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更新日期/Last Update: 2018-04-25