|本期目录/Table of Contents|

[1]杨永,张学军,李寐华,等.微生物肥料对设施长期连作哈密瓜根际土壤真菌群落结构的影响[J].应用与环境生物学报,2018,24(01):68-74.[doi:10.19675/j.cnki.1006-687x.2017.03014]
 YANG Yong,ZHANG Xuejun,LI Meihua,et al.Effects of microbiological fertilizer on rhizosphere soil fungus communities under long-term continuous cropping of protected Hami melon[J].Chinese Journal of Applied & Environmental Biology,2018,24(01):68-74.[doi:10.19675/j.cnki.1006-687x.2017.03014]
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微生物肥料对设施长期连作哈密瓜根际土壤真菌群落结构的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年01期
页码:
68-74
栏目:
研究论文
出版日期:
2018-02-09

文章信息/Info

Title:
Effects of microbiological fertilizer on rhizosphere soil fungus communities under long-term continuous cropping of protected Hami melon
作者:
杨永张学军李寐华王登明王广智张永兵伊鸿平
新疆农业科学院哈密瓜研究中心 乌鲁木齐 830091
Author(s):
YANG Yong ZHANG Xuejun LI Meihua WANG Dengming WANG Guangzhi ZHANG YongbingYI Hongping**
Hami Melon Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
关键词:
微生物肥料高通量测序连作哈密瓜根际真菌多样性
Keywords:
microbiological fertilizer high-throughput sequencing continuous cropping Hami melon rhizosphere fungus diversity
分类号:
S144 : S154.3
DOI:
10.19675/j.cnki.1006-687x.2017.03014
摘要:
长期连作导致哈密瓜土传真菌病害大面积发生,微生物肥料的施用可为防治土传真菌病害发挥积极作用. 应用高通量测序技术深入分析微生物肥料对设施长期连作哈密瓜根际土壤真菌多样性和群落组成的影响. 结果表明,在苗期、开花坐果期和成熟期,微生物肥料处理比对照操作分类单位OTU(Operational taxonomic units)分别高出24.5%、11.3%和82.1%;微生物肥料处理哈密瓜根际真菌物种数、真菌群落丰度和多样性指数,均随生育期呈先降低后增加的变化趋势,而对照处理均表现为持续降低,且在成熟期,各项指数差异显著;主坐标(Principal co-ordinates analysis, PCoA)分析表明微生物肥料处理与对照哈密瓜根际真菌群落结构随生育期推进,其差异越发明显;微生物肥料处理和对照哈密瓜根际优势真菌均主要由子囊菌门(Ascomycota)和担子菌门(Badidiomycota)组成,且以子囊菌门(Ascomycota)种类最多,施微生物肥料处理优势真菌中还存在壶菌门、接合菌门、球囊菌门和一些未明确分类的真菌;样品中丰度排名前35的属共分为A、B、C、D四大类群,A类群和B类群多为致病菌,分别在NM3和NM1含量较高,C类群和D类群多为植物益生菌,分别在M3和M1含量较高. 综上所述,微生物肥料改变了哈密瓜根际优势真菌组成,与对照相比,在苗期和成熟期降低了根际病原真菌数量,且繁衍了大量有益真菌,这可能是微生物肥料发挥促生防病效应,克服或减缓哈密瓜连作障碍的最主要原因. (图5 表1 参35)
Abstract:
Long-term and continuous cropping caused soil-borne fungal disease outbreaks in a large area of Hami melon, and famers suffered enormous economic losses. Microbial fertilizer has played a positive role in preventing and controlling soil fungal diseases. The effect of microbial fertilizer on rhizosphere fungal community structure was analyzed using high-throughput sequencing technologies under seven years continuous cropping of protected Hami melon. Results showed that the operational taxonomic units (OTUs) after the application of microbial fertilizer treatment were 24.5%, 11.3%, and 24.5% more than those in the blank treatment at the seedling stage, blossoming and fruit bearing stage, and mature period, respectively. After microbial fertilizer treatment, the rhizosphere fungal species, fungal community abundance, and diversity index first decreased and then increased along with the growth period; however, these parameters continuously decreased in the blank treatment. Further, their values were significantly higher after microbial fertilizer treatment than in the control during the maturity period. The principal co-ordinate analysis showed that the difference of rhizosphere fungal community structure was more obvious in the growth period between the treatment and blank. The dominant rhizosphere fungal community mainly consisted of Ascomycota and Basidiomycota, in which the fungi belonging to Ascomycota were the most prevalent. Further, some fungi of Chytridiomycota, Glomeromycota, and Zygomycota as well as unclassified fungal species were dominant in the rhizosphere fungal community after microbial fertilizer treatment. The top 35 genera in the samples were divided into four groups, i.e., A, B, C, and D. A and B groups are mainly composed of pathogenic fungi, which are high in NM3 and NM1, and the C and D groups mainly included probiotics, which are high in M3 and M1, respectively. In conclusion, microbial fertilizer could regulate and control the composition of dominant soil fungi. Compared with the blank treatment, application of microbial fertilizer reduced the number of rhizosphere pathogenic fungi and led to the accumulation of some useful fungi, which might account for the effect of microbial fertilizer on facilitating growth, preventing diseases, and overcoming or alleviating continuous cropping obstacles.

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更新日期/Last Update: 2018-02-09