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[1]孟会生,洪坚平,王向英,等.磷细菌肥对采煤塌陷区复垦土壤 放线菌群落的影响*[J].应用与环境生物学报,2016,22(05):911-916.[doi:10.3724/SP.J.1145.2016.03043]
 MENG Huisheng,HONG Jianping**,WANG Xiangying & LI Li.Effect of phosphobacteria fertilizer on soil actinomycetes community in mining subsidence area*[J].Chinese Journal of Applied & Environmental Biology,2016,22(05):911-916.[doi:10.3724/SP.J.1145.2016.03043]
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磷细菌肥对采煤塌陷区复垦土壤 放线菌群落的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年05期
页码:
911-916
栏目:
研究论文
出版日期:
2016-10-25

文章信息/Info

Title:
Effect of phosphobacteria fertilizer on soil actinomycetes community in mining subsidence area*
作者:
孟会生洪坚平王向英栗丽
山西农业大学资源环境学院 太谷 030801
Author(s):
MENG Huisheng HONG Jianping** WANG Xiangying & LI Li
College of Resources and Environmental Science, Shanxi Agriculture University, Taigu 030801, China
关键词:
磷细菌采煤塌陷区放线菌群落高通量测序冗余分析
Keywords:
phosphobacteria mining subsidence area actinomycetes community high?throughput?sequencing redundancy analysis
分类号:
S154.3 : S158.1
DOI:
10.3724/SP.J.1145.2016.03043
摘要:
为了解磷细菌肥对采煤塌陷区复垦土壤放线菌群落的影响,采用Illumina高通量测序的方法,对土壤复垦5年后的放线菌群落结构及多样性进行分析,并结合冗余分析(RDA)研究放线菌群落与土壤化学性质的相关关系. 结果表明:有机肥+磷细菌肥处理可以提高复垦土壤放线菌的数量. 采煤塌陷复垦土壤中放线菌的优势菌群是链霉菌属(Streptomyces)和假诺卡氏菌属(Pseudonocardia),其丰度范围分别为12.28%-15.21%、8.93%-11.49%. 配施磷细菌肥处理可以降低链霉菌属的相对丰度,提高假诺卡氏菌属的相对丰度. 有机肥+磷细菌肥处理能够提高放线菌的Shannon-Wiener和Simpson多样性指数,对Pielou均匀度指数影响较小. RDA结果显示,土壤有机质、有效磷、速效钾、碱解氮是造成复垦土壤放线菌群落丰度和多样性差异的主要原因. 综上认为,有机肥+磷细菌肥处理对复垦土壤放线菌群落的提高作用明显,可以在一定程度上加快土壤的熟化进程. (图2 表6 参26)
Abstract:
In order to study the effect of phosphorus bacteria fertilizer on actinomycetes community in coal mining subsidence area, the Illumina high-throughput sequencing method was used to analyze actinomycetes community structure and diversity after 5 years of soil reclamation. The relationship between actinomycetes community and chemical properties was investigated using redundancy analysis (RDA). The results showed the treatment of organic fertilizer + phosphorus bacteria fertilizer could increase the number of actinomycetes in reclaimed soil. The dominant actinomyces community of reclaimed soil in the coal mining subsidence area was Streptomyces (relative abundance 12.28% – 15.21%) and Pseudonocardia (relative abundance 8.93% – 11.49%). Phosphorus bacteria fertilizer treatment could reduce the relative abundance of Streptomyces and improve that of Pseudonocardia. Organic fertilizer + phosphorus bacteria fertilizer treatment could improve the diversity index of Shannon-Wiener and Simpson but had little effect on Pielou evenness index. Results of RDA showed that soil organic matter, available phosphorus, available potassium and available nitrogen were the major factors causing differences of abundance and diversity of actinomyces community in reclaimed soil.In summary, organic fertilizer + phosphorus bacteria fertilizer significantly improved actinomyces community and speeded up the ripening process of reclaimed soil in some degree.

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更新日期/Last Update: 2016-10-25