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[1]王君,杜秉海,马海林,等.纺锤形芽孢杆菌L13对蓝莓生长及根际土壤 生物学特性的影响*[J].应用与环境生物学报,2016,22(05):917-925.[doi:10.3724/SP.J.1145.2015.12014]
 WANG Jun,DU Binghai**,et al.Effect of Bacillus fusiformis L13 on blueberry growth and rhizosphere soil biological characteristics*[J].Chinese Journal of Applied & Environmental Biology,2016,22(05):917-925.[doi:10.3724/SP.J.1145.2015.12014]
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纺锤形芽孢杆菌L13对蓝莓生长及根际土壤 生物学特性的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年05期
页码:
917-925
栏目:
研究论文
出版日期:
2016-10-25

文章信息/Info

Title:
Effect of Bacillus fusiformis L13 on blueberry growth and rhizosphere soil biological characteristics*
作者:
王君杜秉海马海林刘方春丁延芹姚良同李丽
1山东农业大学生命科学学院,山东省农业微生物重点实验室 泰安 271018 2山东省林业科学研究院 济南 250014
Author(s):
WANG Jun1 2 DU Binghai1** MA Hailin2 LIU Fangchun2 DING Yanqin1 YAO Liangtong1 & LI Li2
1Shandong Key Laboratory of Agricultural Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China 2Shandong Academy of Forest, Ji’nan 250014, China
关键词:
纺锤形芽孢杆菌单一菌剂复合微生物肥料蓝莓土壤细菌群落结构
Keywords:
Bacillus fusiformis single species inoculant compound microbial fertilizer blueberry soil bacterial community
分类号:
Q939.96 : S144
DOI:
10.3724/SP.J.1145.2015.12014
摘要:
以八年生蓝莓品种“蓝丰”为供试植株,通过田间试验研究添加纺锤形芽孢杆菌L13在单一菌剂和复合微生物肥料中对植株生长及根际土壤的影响. 试验设4个处理,其中单一菌剂即L13(P),以不施肥处理作为对照(CK);以灭菌后的腐熟鸡粪作为吸附载体接种酵母菌DY3和乳酸菌DY4作为普通生物肥(OF),在此基础上进一步接种L13作为复合微生物肥料(PF),以OF作为对照,分别研究单一菌剂和复合微生物肥料对蓝莓叶片酶活、果实品质、植株养分、根际土壤酶活和理化性质以及细菌群落结构的影响. 结果表明:含L13的两种肥料都显著降低了果实中可滴定酸含量,提高了植株全氮、全钾含量以及土壤碱解氮、全氮、有机质含量,促进了蓝莓生长;L13在单一菌剂中的作用更为明显,和对照相比,叶片中丙二醛含量降低61.9%,果实中花青苷含量提高21.68%,可滴定酸含量降低13.61%,植株全氮、全磷、全钾分别提高4.12%、10.27%、8.63%,土壤蔗糖酶、酸性磷酸酶活性分别提高107.59%、21.36%,土壤碱解氮、有效磷、速效钾、全氮、有机质含量分别提高了49.66%、31.28%、21.95%、63.83%、84.54%,土壤pH降低,并且两个处理间各指标都达到了显著性差异. 运用Illumina Miseq高通量测序分析根际土壤细菌群落结构发现,L13作为单一菌剂显著降低了土壤细菌菌群丰度和多样性,而在复合微生物肥料中菌群丰度和多样性增加;单一菌剂和复合微生物肥料都增加了变形菌门和厚壁菌门的比例,单一菌剂还增加了放线菌门的比例,复合微生物肥料增加了拟杆菌门的比例;放线菌目、芽孢杆菌目等的比例也有所增加;L13在单一菌剂中对蓝莓根际细菌群落结构的影响最为明显,厚壁菌门的改变是造成结构差异的主要因素. 综上,纺锤形芽孢杆菌L13在两种微生物肥料中都能发挥其促生作用,在单一菌剂中作用更显著,土壤细菌群落结构的改变同蓝莓生长、土壤肥力状况存在一定的相关性,这为PGPR与植物根际的相互作用研究以及微生物肥料的研制提供了理论基础. (图5 表7 参36)
Abstract:
The aim of this research was to determine the effect of Bacillus fusiformis L13 on blueberry growth and rhizosphere soil when used as single species inoculant and compound microbial fertilizer. A field experiment was conducted using the blueberry breed Bluecrop. We set four treatments in this research, including no fertilizer as a control (CK) for the fermentation liquid of L13 as single species inoculant (P), the normal biological fertilizer of mixture of yeast DY3, lactobacillus DY4 and decomposed chicken manure (OF) as a control for the compound microbial fertilizer (PF) of mixture of OF and L13. Then we measured the enzyme activity in leaves, fruit quality, nutrient content in plant, enzyme activity and physicochemical property of the rhizosphere soil, and the bacterial community of soil. The B. fusiformis L13 in the single species inoculant and the compound microbial fertilizer significantly decreased the titratable acid content, increased the content of total nitrogen and total potassium, and increased the available nitrogen, the total nitrogen, and the organic matter in the soil. We found L13 promoted the growth of blueberry in both microbial fertilizers, being more effective in single application than in the compound one. Compared with CK, the P treatment had 61.9% lower leave MDA content, 21.68% higher fruit anthocyanin, 13.61% lower fruit titratable acid, and 4.12%, 10.27%, 8.63%, respectively, higher contents of total nitrogen, phosphorus, and potassium. The activity of soil invertase was 107.59% higher and the activity of soil acid phosphatase 21.36% higher. In addition, the total nitrogen, the organic matter, and the available nitrogen, phosphorus, and potassium was 63.83%, 84.54%, 49.66%, 31.28%, 21.95%, respectively, higher in P treatment. The pH of the soil was significantly lower with L13 than the CK. We used a next-generation sequencing method, Illumina Miseq detecting the bacterial community of rhizosphere soil. And the results showed that the single species inoculant reduced the bacterial community richness and diversity compared with CK, which increased by using compound fertilizer compared with OF. L13 in both microbial fertilizers increased the proportion of Proteobacteria and Firmicutes, in addition to increasing the percentage of Actinobacteria in single inoculation increasing the percentage of Baceroidetes in compound application. It also increased the amount of beneficial bacteria including Actinomycetales and Bacillales. The effect of L13 was more obvious when used as single microbial fertilizer, with the change of Firmicutes as a very important factor. The results indicated that B. fusiformis L13 used as single species inoculant or compound microbial fertilizer can promote the growth of blueberry, being more effective in single fertilizer. There is some correlation between the change of the bacterial community of rhizosphere soil and the growth of blueberry, as well as soil fertility.

参考文献/References:

1 刘克宁, 李公存, 顾海燕, 苏佳明, 赵明. 蓝莓核心种质资源评价[J]. 山东农业科学, 2011 (1): 20-23 [Liu KN, Li GC, Gu HY, Su JM, Zhao M. Evaluation of key blueberry germplasm resources [J]. Shandong Agric Sci, 2011 (1): 20-23] 2 修英涛, 常凤英, 姜河, 杨伟力, 侯利军. 我国蓝莓(越桔)研究现状及发展措施[J]. 辽宁农业科学, 2003 (3): 21-23 [Xiu YT, Chang FY, Jiang H, Yang WL, Hou LJ. Research status and development measures of bluberry (vaccinium) in our country [J]. Liaoning Agric Sci, 2003 (3): 21-23] 3 张铭浩, 杨晶, 许孝瑞. 新兴果树蓝莓的发展前景与栽培技术[J]. 落叶果树, 2011 (2): 20-22 [Zhang MH, Yang J, Xu XR. Development prospects and cultivation techniques of the rising blueberry [J]. Dec Fruits, 2011 (2): 20-22] 4 李丽敏, 吴林, 郝庆升, 李亚东. 中国蓝莓市场现状及产业发展对策研究[J]. 中国果树, 2011 (3): 70-73 [Li LM, Wu L, Hao QS, Li YD. Research of market status and development strategy of blueberry in China [J]. Chin Fruits, 2011 (3): 70-73] 5 魏殿文. 寒温带蓝莓栽培土壤综合调控研究[D]. 哈尔滨: 东北林业大学, 2012 [Wei DW. Study on comprehensive regulation of soils for cultivating blueberry in cold temperate zone [D]. Haerbin: Northeast Forestry University, 2012] 6 Gaskins MH, Albrecht SL, Hubbell DH. Rhizosphere bacteria and their use to increase plant productivity [J]. Agric Ecosyst Environ, 1985, 12 (2): 99-106 7 Thomas JB, Athony C, Schrolh MN. Beneficial plant bacteria [J]. Crit Rev Plant Sci, 1984, 2 (1): 1-20 8 Glick BR. The enhancement of plant growth by free-living bacteria [J]. Can J Microbiol, 1995, 41 (2): 109-117 9 井大炜, 马海林, 刘方春, 杜振宇, 马丙尧. 微生物肥料在经济林中的应用研究现状及展望[J]. 生物灾害科学, 2013, 36 (4): 443-447 [Jing DW, Ma HL, Liu FC, Du ZY, Ma BY. Research status of bio-fertilizers applied in economic forests [J]. Biol Dis Sci, 2013, 36 (4): 443-447] 10 刘鹏, 刘训理. 中国微生物肥料的研究现状及前景展望[J]. 农学学报, 2013, 3 (3): 26-31 [Liu P, Liu XL. Current research status and prospect of microbial fertilizer in China [J]. J Agric, 2013, 3 (3): 26-31] 11 刘刊, 耿士均, 王波, 商海燕. 微生物肥料研究进展[J]. 安徽农业科学, 2011, 39 (22): 13445-13447 [Liu K, Geng SJ, Wang B, Shang HY. Reseach progress of microbial fertilizer [J]. J Anhui Agric Sci, 2011, 39 (22): 13445-13447] 12 Smalla K, Oros-Sichler M, Milling A, Heuer H, Baumgarte S, Becker R, Neuber G, Kropf S, Ulrich A, Tebbe CC. Bacterial diversity of soils assessed by DGGE, T-RFLP and SSCP fingerprints of PCR-amplified 16s rRNA gene fragments: do the different methods provide similar results? [J]. J Microbiol Meth, 2007, 69 (3): 470-479 13 张汉波, 段昌群, 屈良鹄. 非培养方法在土壤微生物生态学研究中的应用[J]. 生态学杂志, 2003, 22 (5): 131-136 [Zhang HB, Duan CQ, Qu LH. Culture independent methods for studies on microbial ecology of soils [J]. J Ecol, 2003, 22 (5): 131-136] 14 秦楠, 栗东芳, 杨瑞馥. 高通量测序技术及其在微生物学研究中的应用[J]. 微生物学报, 2011, 51 (4): 445-457 [Qin N, Li DF, Yang RF. Next-generation sequencing technology and its application in microbiological research [J]. Acta Microbiol Sin, 2011, 51 (4): 445-457] 15 楼骏, 柳勇, 李延. 高通量测序技术在土壤微生物多样性研究中的研究进展[J]. 中国农学通报, 2014, 30 (15): 256-260 [Lou J, Liu Y, Li T. Review of high-throughput sequencing techniques in studies of soil microbial diversity [J]. Chin Agric Sci Bull, 2014, 30 (15): 256-260] 16 陈波, 马海林, 刘方春, 杜秉海. 生物有机肥对樱桃生长及根际土壤生物学特征的影响[J]. 水土保持学报, 2013, 27 (2): 267-271 [Chen B, Ma HL, Liu FC, Du BH. Effects of bio-fertilizer on Cerasus pseudocersus growth and rhizosphere soil biological characteristics [J]. J Soil Water Conserv, 2013, 27 (2): 267-271] 17 郑炳松. 现代植物生理生化研究技术[M]. 北京: 气象出版社, 2006 [Zheng BS. Reseach techniques in contemporary plant physiology and biochemistry [M]. Beijing: China Meteorological Press, 2006] 18 鲍士旦. 土壤农化分析[M]. 北京: 中国农业出版社, 2000 [Bao SD. Soil agro-chemistry analysis [M]. Beijing: China Agricultural Press, 2000] 19 郝建军, 康宗利, 于洋. 植物生理学实验技术[M]. 北京: 化学工业出版社, 2006: 159-160 [Hao JJ, Kang ZL, Yu Y. Techniques in plant physiology [M]. Beijing: Chemical industry publishing house, 2006: 159-160] 20 孙磊. 植物营养学实验[M]. 北京: 北京大学出版社, 2012 [Sun L. Experiment of Plant Nutrition [M]. Beijing: Peking University Press, 2012] 21 关松荫. 土壤酶及其研究方法[M]. 北京: 农业出版社, 1986 [Guan SY. Soil Enzyme and Its Research Methods [M]. Beijing: Agricultural publishing House, 1986] 22 曹丹, 宗良纲, 肖峻,张倩, 赵妍. 生物肥对有机黄瓜生长及土壤生物学特性的影响[J]. 应用生态学报, 2010, 21 (10): 2587-2592 [Cao D, Zong LG, Xiao J, Zhang Q, Zhao Y. Effects of bio-fertilizer on organically cultured cucumber growth and soil biological charac-teristics [J]. Chin J Appl Ecol, 2010, 21 (10): 2587-2592] 23 孙运杰, 马海林, 刘方春, 孙蕾, 王君, 刘春生. 生物肥对蓝莓根际土壤微生物学特性及土壤肥力的影响[J]. 水土保持学报, 2015, 29 (3): 167-177 [Sun YJ,Ma HL, Liu FC, Sun L, Wang J, Liu CS. Effects of bio-fertilizer on microbial properties and soil fertility in blueberry rhizosphere soil [J]. J Soil Water Conserv, 2015, 29 (3): 167-177] 24 张以顺, 黄霞, 陈云凤. 植物生理学实验教程[M]. 北京: 高等教育出版社, 2009 [Zhang YS, Huang X, Chen XF. Experimental course of plant physiology [M]. Beijing: Higher education press, 2009] 25 刘旭, 王昊, 谭军. 水分胁迫对紫薇生长及生理生化特征的影响[J]. 江苏农业科学, 2015, 43 (10): 239-241 [Liu X, Wang H, Tan J. Effetts of water stress on lagerstroemia indica growth and its physiology and biochemistry [J]. Jiangsu Agric Sci, 2015, 43 (10): 239-241] 26 高彦波, 翟鹏辉, 谭德远. 土壤酶活性与土壤肥力的关系研究进展[J]. 安徽农业科学, 2015, 43 (6): 100-101, 183 [Gao YB, Zhai PH, Tan DY. Research progress of relationship between soil enzyme activity and soil fertility [J]. J Anhui Agric Sci, 2015, 43 (6): 100-101, 183] 27 张焱华, 吴敏, 何鹏, 佘贵连, 吴炳孙, 韦家少. 土壤酶活性与土壤肥力的关系研究进展[J]. 安徽农业科学, 2007, 35 (34): 11139-11142 [Zhang YH, Wu M, He P, She GL, Wu BS, Wei JS. Research advance of the relationship between soil enzyme activity and soil fertility [M]. J Anhui Agric Sci, 2007, 35 (34): 11139-11142] 28 Girvan MS, Campbel CD, Killham K, Prosser JI, Glover LA. Bacterial diversity promotes community stability and functional resilience after perturbation[J]. Environ Microbiol, 2005, 7 (3): 301-313 29 吴江利, 罗学刚, 李宝强, 司慧. 微生物菌肥作用下荒漠土壤微生物群落结构和功能研究[J]. 中国农学通报, 2015, 31 (9): 216-223 [Wu JL, Luo XG, Li BQ, Si H. Researches on microbial community structure and function in desert soil under microbial fertilizer [J]. Chin Agric Sci Bull, 2015, 31 (9): 216-223] 30 邬奇峰, 陆扣萍, 毛霞丽, 秦华, 王海龙. 长期不同施肥对农田土壤养分与微生物群落结构的影响[J]. 中国农学通报, 2015, 31 (5): 150-156 [Wu QF, Lu KP, Mao XL, Qin H, Wang HL. Responses of soil nutrients and microbial biomass and community composition to long-term fertilization in cultivated land [J]. Chin Agric Sci Bull, 2015, 31 (5): 150-156] 31 王菲, 袁婷, 谷守宽, 王正银. 有机无机缓释复合肥对不同土壤微生物群落结构的影响[J]. 环境科学, 2015, 36 (4): 1461-1467 [Wang F, Yuan T, Gu SK, Wang ZY. Effects of organic and inorganic slow-release compound fertilizer on different soils microbial community structure [J]. Chin J Envir Sci, 2015, 36 (4): 1461-1467] 32 罗欢. 芽孢杆菌对植物的促生和耐盐作用及其相关机制的研究[D]. 江苏南京: 南京农业大学, 2013 [Luo H. Study of growth promoting and salt tolerance of Bacillus spp. plant and the research of underling mechanism [D]. Nanjing, Jiangsu: Nanjing Agricultural University, 2013] 33 Xia Y, Xu Q, Lin Y, Chen ZZ, Kong FY, Zhang CX. Research progress of mechanism of action of plant growth promoting rhizobacteria [J]. Agric Sci Technol, 2014, 15 (1): 87-90,110 34 孙海新. 茶树根际促生细菌的研究[D]. 泰安: 山东农业大学, 2004 [Sun HX. Studies on plant growth-promoting rhizobacteria of tea rhizosphere [D]. Taian: Shandong agricultural university, 2004] 35 康铸慧, 王磊, 郑广宏, 周琪. 微生物产氢研究的进展[J]. 工业微生物, 2005, 35 (2): 41-49 [Kang ZH, Wang L, Zheng GH, Zhou Q. Research progress in hydrogen production microbiological processes [J] Ind Microbiol, 2005, 35 (2): 41-49] 36 刘方春, 邢尚军, 马海林, 杜振宇, 马丙尧, 陈波, 杜秉海. PGPR生物肥对甜樱桃(Cerasus pseudocerasus)根际土壤生物学特性的影响 [J]. 应用与环境生物学报, 2012, 18 (5): 722-727 [Liu FC, Xing SJ, Ma HL, Du ZY, Ma BY, Chen B, Du BH. Effect of PGPR fertilizer on biological characteristics in Cerasus pseudocerasus rhizosphere [J]. Chin J Appl Environ Biol, 2012, 18 (5): 722-727]

更新日期/Last Update: 2016-10-25