|本期目录/Table of Contents|

[1]伍思宇 周志如 尤 青 张文飞 徐 达 李特伦 姚 琦 张起畅 吴红萍 王锐萍?.海南东寨港红树林聚磷菌的筛选及其特性?[J].应用与环境生物学报,2016,22(03):397-401.[doi:10.3724/SP.J.1145.2015.11026]
 WU Siyu,ZHOU Zhiru,YOU Qing,et al.Screening and characterization of soil phosphate accumulating bacteria from Dongzhaigang mangrove wetlands in Hainan, China*[J].Chinese Journal of Applied & Environmental Biology,2016,22(03):397-401.[doi:10.3724/SP.J.1145.2015.11026]
点击复制

海南东寨港红树林聚磷菌的筛选及其特性?()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年03期
页码:
397-401
栏目:
研究论文
出版日期:
2016-06-25

文章信息/Info

Title:
Screening and characterization of soil phosphate accumulating bacteria from Dongzhaigang mangrove wetlands in Hainan, China*
作者:
伍思宇 周志如 尤 青 张文飞 徐 达 李特伦 姚 琦 张起畅 吴红萍 王锐萍?
热带动植物生态学省部共建教育部重点实验室,海南师范大学生命科学学院 海口 571158
Author(s):
WU Siyu ZHOU Zhiru YOU Qing ZHANG Wenfei XU Da LI Telun YAO Qi ZHANG Qichang WU Hongping & WANG Ruiping**
Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou 571158, China
关键词:
水体富营养化红树林聚磷菌菌株筛选不动杆菌属
Keywords:
water eutrophication mangrove phosphorus accumulating organisms screening Acinetobacter
分类号:
X172
DOI:
10.3724/SP.J.1145.2015.11026
摘要:
海南东寨港红树林自然保护区生态环境与聚磷菌的聚磷过程好氧-厌氧交替环境高度一致. 为获得高效聚磷菌,本研究随机采集该区域134份淤泥样品,通过BCIP蓝白斑筛选法、异染颗粒和PHB颗粒染色以及测定聚磷能力相结合的方法进行筛选. 总共获得185株初筛菌株,其中42株菌的聚磷能力达20%以上,最高可达69.5%,有效聚磷菌株分离率为22.7%. 利用Biolog微生物鉴定系统和16S rRNA基因序列分析鉴定菌株,发现所筛聚磷菌多为不动杆菌属(Acinetobacter),也有芽孢杆菌属(Bacillus)、寡养单胞菌属(Stenotrophomonas)和假单孢菌属(Pseudomonas),并且表现出一定的差异性. 本研究表明东寨港红树林具有丰富的聚磷微生物资源,可为修复磷富营养化水体恢复生态平衡提供有效的候选菌种. (图3 表3 参28)
Abstract:
Dongzhaigang Mangrove Forest Nature Reserve, located in northeastern Hainan Island, is the major mangrove wetlands in China. The mangroves are evergreen trees that grow along the tropical or sub-tropical tidal shores or river estuaries. At high tide, they are totally submerged or only their tops are visible; when the tide recedes, they show themselves as dense thickets. The mangroves are periodically submerged by seawater, which have the same eco-environment of accumulation of large quantities of polyphosphate within their cells and the removal of phosphorus by Phosphorus Accumulating Organisms (PAOs). Thus, PAOs are selectively enriched in the bacterial community within the activated sludge of mangrove wetlands. In the present study, we screened 134 sludge samples isolated from the mangrove wetlands in Dongzhaigang and obtained 185 isolates of PAOs through the methods of BCIP blue-and white-colored screening, Albert staining and Nile Blue staining, which determined if the polyP or polyhydroxybutyrate (PHB) is on accumulation. The results showed a phosphorus removal rate of 42 PAOs as higher than 20%, and the maximum removal efficiency for phosphorus higher than 80%. Biolog phenotype microarrays and 16S rDNA sequences analysis were conducted to identify the isolates of PAOs. The result showed the populations of PAOs constituted by the bacterial genus of Acinetobacter sp., Bacillus sp., Stenotrophomonas sp., Pseudomonas sp. genera etc., with Acinetobacter being dominant in the entire communities. In our study, Dongzhaigang Mangrove Forest Nature Reserve is rich in PAO strains resources with good diversity. In this study, PAOs isolated from Dongzhaigang Mangrove Forest Nature Reserve are important in enhanced biological phosphorus removal and can be a candidate strain for preventing eutrophication in water bodies.

参考文献/References:

1 Yang XE, Wu X, Hao HL, He ZL. Mechanisms and assessment of water eutrophication [J]. J Zhejiang Univ B, 2008, 3 (3): 197-209 2 林晓, 刘蜻, 徐厚坤. 富营养化水体中磷的控制方法初探[J]. 城镇供水, 2003, 2 (1): 29-30 3 Abell JM, ?zkundakci D, Hamilton DP. Nitrogen and phosphorus limitation of phytoplankton growth in New Zealand lakes: implicatios for eutrophication control [J]. Ecosystems, 2010, 13 (7): 966-977 4 郑金伟, 冉炜, 钟增涛, 何健. 增强型生物除磷过程中聚磷酸盐积累微生物的研究进展[J]. 应用生态学报, 2004, 15 (8): 1487-1490 [Zheng JW, Ran W, Zhong ZT, He J. Research advance in polyphosphate-accumulating microorganisms in enhanced biological phosphorus removal process. Chin J Appl Ecol, 2004, 15 (8): 1487-1490] 5 Seralathan P, Rajkumar MS, Sunilkumar V, Anandaraj N. Interstitial water chemistry of mangrove sediments, Kerala [J]. J Geol Soc India, 2006, 68 (2): 251-258 6 李玲玲, 郑西来, 吴俊文, 李梅, 赵淑梅. 亚硝酸盐对聚磷菌好氧摄磷的影响研究[J]. 环境科学, 2006, 27 (8): 1574-1579 [Li LL, Zheng XL, Wu JW, Li M, Zhao SM. Effect of nitrite on aerobic phosphate uptake by phosphate accumulating organisms. Environ Sci, 2006, 27 (8): 1574-1579] 7 马斌, 彭永臻, 王淑莹, 葛士建, 杨莹莹, 祝贵兵. 强化生物除磷系统中聚磷菌菌群特性[J]. 化工学报, 2010, 61 (5): 1282-1285 [Ma B, Peng YZ, Wang SY, Ge SJ, Yang YY, Zhu GB. Characterization of polyphosphate-accumulating bacteria community structure in enhanced biological phosphorus removal reactor [J]. CIESC J, 2010, 61 (5): 1282-1285] 8 李海峰, 李志建, 屈建航. 高效聚磷菌的分离、筛选与构建的研究进展[J]. 生物技术, 2012, 22 (4): 93-97 [Li HF, Li ZJ, Qu JH. Research progress of isolation, screening and building of High-efficient polyphosphate-accumulating organisms [J]. Biotechnology, 2012, 22 (4): 93-97] 9 Fuhs GW, Chen M. Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater [J]. Micmb Ecol, 1975(2): 119-138 10 Meganck M, Malnou D, Flohie PL, Faup GM, Rovel JM. The importance of the acidogenic microflora in biological phosphorus removal [J]. Waterence Technol, 1985, 17 (11-12): 199-212 11 Cai TM, Guan LB, Chen LW, Cai S, Li XD, Cui ZL, Li SP. Enhanced biological phosphorus removal with Pseudomonas putida GM6 from activated sludge [J]. Pedosphere, 2007, 17 (5): 624-629 12 刘亚男, 于水利, 薛罡, 赵方波, 郭思远. 聚磷茵PAO1-1的筛选及除磷特性仁[J]. 中国给水排水, 2005, 21 (10): 13-17 [Liu YN, Yu SL, Xue G, Zhao FB, Guo SY. Study on separation and screening of phosphate accumulating bacterial strain PAO1-1 and characteristics of phosphorus removal. China Water Wastewater, 2005, 21 (10): 13-17 13 张培玉, 孙梦, 张晨. 环境因素对3株嗜盐聚磷菌除磷性能的影响[J]. 山东大学学报(理学版), 2015, 50 (5): 88-50 [Zhang PY, Sun M, Zhang C. Environmental factors influence on phosphorus removal of three halophilic phosphate accumulating bacteria strains [J]. J Shandong Univ (Nat Sci), 2015, 50 (5): 88-50] 14 Lin P. Mangrove Ecosystem in China [M]. Beijing: Science Press, 1999: 21-23, 67-68 15 任健, 阎冰, 洪葵. 海南东寨港红树林不同植被土壤微生物群落结构比较[J]. 微生物学报, 2012, 52 (6): 736-743 [Ren J, Yan B, Hong K. Comparison of bacterial and archaeal community of margrove soil under different vegetion in Dongzhaigang, Hailan Island. Acta Microbiol Sin, 2012, 52 (6): 736-743] 16 陈桂珠. 研究保护和开发利用红树林生态系统[J]. 生态科学, 1991, 1: 116-119 17 曹启民, 郑康振, 陈耿, 陈桂珠. 红树林生态系统微生物学研究进展[J]. 生态环境, 2008, 17 (2): 839-845 [Cao QM, Zheng KZ, Chen G, Chen GZ. A review of Studies on microsiology of mangrove ecosystems Ecol Environ, 2008, 17 (2): 839-845] 18 Dahdouh-Guebas F, Collin S, Lo Seen D, Ronnback P, Depommier D, Ravishankar T, Kocdaln N. Analysing ethnobotanical and fishery-related importance of mangroves of the East-Godavari Delta (Andhra Pradesh, India) for conservation and management purposes [J]. J Ethnobiol Ethnomed, 2006, 8 (2): 24 19 Kuroda A, Takiquchi N, Gotanda T, Nomura K, Kato J, Ikeda T, Ohtake H. A simple method to release polyphosphate from activated sludge for phosphorus reuse and recycling [J]. Biotechnol Bioeng, 2002, 78 (3): 333-338 20 Neidhardt FC, Bloch PL, Smith DF. Culture medium for enterobacteria [J]. J Bacteriol, 1974, 119 (3): 736-747 21 Morohoshi T, Yamashita T, Kato J, Ikeda T, Takiquchi N, Ohtake H, Kuroda A. A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater [J]. J Biosci Bioeng, 2003, 95 (6): 637-640 22 陈接锋, 许旭萍, 佘晨兴, 李惠珍. 合成聚β-羟基丁酸(PHB)鞘细菌的分离、鉴定与筛选[J]. 工业微生物, 2003, 33 (4): 23-26 [Chen JF, Xu XP, She CX, Li HZ. Isolation, identification and screening of Sheathed bacteria synthesizing poly-hyclorxybntyric acid (PHB). Ind Microbiol, 2003, 33 (4): 23-26] 23 刘长秀, 罗海华, 周红, 江丽芳, 李彩霞, 郑树森. 白喉杆菌异染颗粒Neisser法染色的新探讨[J]. 热带医学杂志, 2006, 6 (2): 165-166 [Liu CX, Luo HH, Zhou H, Jiang LF, Li CX, Zheng SS. Neisser stain of the metachromatic granules of C.diphtheriae [J]. J Trop Med, 2006, 6 (2): 165-166 24 国家环境保护总局. 水和废水监测分析方法[M]. 4版. 北京: 中国环境科学出版社, 2002 25 蔡妙英, 东秀珠. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001 26 谢家仪, 董光军, 刘振英. 扫描电镜的微生物样品制备方法[J]. 电子显微学报, 2005, 24 (4): 440-440 27 程池, 杨梅, 李金霞, 姚粟, 胡海蓉. Biolog微生物自动分析系统-细菌鉴定操作规程的研究[J]. 食品与发酵工业, 2006, 32 (5): 50-54 [Cheng C, Yang M, Li JX, Yao S, Hu HR. Biolog microbial ideritificatron system-study on the operating regulation of Bacteria iden tification. Food Ferment Ind, 2006, 32 (5): 50-54] 28 Devereux R, He SH, Doyle CL, Orkland S, Stahl DA, Legall J, Whitman WB. Diversity and origin of Desulfovibrio species: phylogenetic definition of a family. J Bacteriol, l990, 172 (7): 3609-3619

相似文献/References:

[1]郑文教,王文卿,林鹏.九龙江口桐花树红树林对重金属的吸收与累积[J].应用与环境生物学报,1996,2(03):207.
 Zheng Wenjiao,Wang Wenqing,Lin Peng.ABSORPTION AND ACCUMULATION OF HEAVY METALS IN AEGICERAS CORNICULATUM MANGROVE FOREST IN THE ESTUARY OF THE JIULONG RIVER IN FUJIAN[J].Chinese Journal of Applied & Environmental Biology,1996,2(03):207.
[2]肖静,许静,谢庶洁,等.红树林放线菌的分离及其抗菌和抗肿瘤细胞活性[J].应用与环境生物学报,2008,14(02):244.
 XIAO Jing,et al..Isolation of Mangrove Actinomycetes and Their Antagonistic Activities[J].Chinese Journal of Applied & Environmental Biology,2008,14(03):244.
[3]甘慧媚,李靖,谭凤仪,等.深圳福田红树林底栖纤毛虫的群落生态学[J].应用与环境生物学报,2010,16(03):363.[doi:10.3724/SP.J.1145.2010.00363]
 GAN Huimei,LI Jing,TAM NORA Fengyi,et al.Community Ecology of Benthic Ciliates in Futian Mangroves, Shenzhen, China[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):363.[doi:10.3724/SP.J.1145.2010.00363]
[4]洪美玲,李春瑜,王力军,等.急性铜胁迫对海陆蛙蝌蚪抗氧化效应及金属解毒能力的影响[J].应用与环境生物学报,2015,21(03):494.[doi:10.3724/SP.J.1145.2014.11012]
 HONG Meiling,LI Chunyu,WANG Lijun,et al.Effect of acute copper stress on antioxidant and metal detoxification ability of the tadpole of crab eating frog (Fejervarya cancrivora)[J].Chinese Journal of Applied & Environmental Biology,2015,21(03):494.[doi:10.3724/SP.J.1145.2014.11012]
[5]姚琦,尤青,李媛宏,等.海南东寨港红树林土壤微生物功能多样性[J].应用与环境生物学报,2017,23(05):857.[doi: 10.3724/SP.J.1145.2016.11001]
 YAO Qi,YOU Qing,LI Yuanhong,et al.Functional diversity of soil microorganisms in the soil in Dongzhaigang mangrove wetlands in Hainan[J].Chinese Journal of Applied & Environmental Biology,2017,23(03):857.[doi: 10.3724/SP.J.1145.2016.11001]

更新日期/Last Update: 2016-06-25