|本期目录/Table of Contents|

[1]苏炜华# 黄 珑# 黄 宁 刘 峰 苏亚春 肖新换 凌 辉 阙友雄.甘蔗细胞色素P450还原酶基因的RT-PCR扩增与表达分析[J].应用与环境生物学报,2016,22(02):173-178.[doi:10.3724/SP.J.1145.2015.07029]
 SU Weihua#,HUANG Long#,HUANG Ning,et al.RT-PCR amplification and expression analysis of a cytochrome P450 reductasegene from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2016,22(02):173-178.[doi:10.3724/SP.J.1145.2015.07029]
点击复制

甘蔗细胞色素P450还原酶基因的RT-PCR扩增与表达分析()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年02期
页码:
173-178
栏目:
研究论文
出版日期:
2016-04-25

文章信息/Info

Title:
RT-PCR amplification and expression analysis of a cytochrome P450 reductasegene from sugarcane
作者:
苏炜华# 黄 珑# 黄 宁 刘 峰 苏亚春 肖新换 凌 辉 阙友雄
福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室/国家甘蔗产业技术研发中心 福州 350002
Author(s):
SU Weihua# HUANG Long# HUANG Ning LIU Feng SU Yachun XIAO Xinhuan LING Hui &QUE Youxiong**
Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Ministry of Agriculture, Fujian Agriculture and Forestry University / SugarcaneResearch & Development Center, China Agricultural Technology System, Fuzhou 350002, China
关键词:
甘蔗ScCPR450基因亚细胞定位分析基因表达模式分析胁迫
Keywords:
sugarcane ScCPR450 gene subcellular localization analysis gene expression pattern analysis stress
分类号:
S566.103.4 : Q78
DOI:
10.3724/SP.J.1145.2015.07029
摘要:
细胞色素P450基因在电子传递链、次生代谢物质合成和对外源化学药物毒性降解中发挥着重要作用,为了深入了解该基因在甘蔗中的功能,究通过RT-PCR扩增获得甘蔗细胞色素P450还原酶基因的cDNA全长序列,命名为ScCPR450(GenBank Accession Number:KR864841). 该基因全长999 bp,含有744 bp的完整开放阅读框,编码247个氨基酸. 亚细胞定位结果显示,ScCPR450蛋白分布于细胞质中,与生物信息学预测结果相符. qRT-PCR表达分析表明,该基因在甘蔗中组成型表达,但有组织特异性,芽中表达量最高,其次是叶,而皮中表达量最低. 在脱落酸(ABA)、水杨酸(SA)、茉莉酸甲酯(MeJA)、聚乙二醇(PEG)和氯化铜(CuCl2)胁迫诱导过程中,该基因的表达量呈现不同变化模式,其中SA胁迫6 h下,ScCPR450基因的表达量最高,约为对照的12.21倍;在PEG胁迫下,ScCPR450基因的表达量上调且表达量稳定,推测ScCPR450基因在甘蔗响应生物和非生物胁迫中发挥一定的作用. 本研究可为该基因家族其它成员的克隆以及深入解析该基因的功能特性奠定基础,进而为基于基因工程技术对甘蔗品种进行定向改良提供基因资源. (图4 参44)
Abstract:
Cytochrome P450 gene plays an important role in the electron transport chain, secondary metabolite synthesis anddegradation of exogenous chemical drug toxicity. In order to understand the function of this gene in sugarcane, this study usedthe EST CF576130.1 with homologs to a sugarcane cytochrome P450 reductase gene as a probe, performed electric cloningtechnology combined with RT-PCR amplification, and obtained the full length cDNA sequence of sugarcane cytochrome P450reductase gene termed as ScCPR450 (GenBank Accession Number:KR864841). Bioinformatics analysis revealed that the lengthof ScCPR450 was 999 bp, which contained a complete open reading frame with a length of 744 bp, encoding 247 amino acidresidues. Subcellular localization showed that ScCPR450 was distributed in the cytoplasm which was in consistence with thebioinformatics prediction result. The qRT-PCR analysis revealed that ScCPR450 was constitutively expressed and had tissuespecificexpression in sugarcane, with the highest expression level in the bud, followed by the leaf and the lowest expression inthe epidemis. At the same time, the expression patterns of ScCPR450 were different after challenging with ABA, SA, MeJA,PEG and CuCl2. Under the SA stress for 6 h, the expression level of ScCPR450 was the highest, about 12.21 times more thanthat in control. Under the stress of PEG, the transcripts of ScCPR450 raised and remained stable. The results suggested thatScCPR450 played a role in response to biotic and abiotic stresses in sugarcane. This study lays a certain foundation for thecloning of other members of CPR family genes and also for further analysis of the gene functional characteristics, whichultimately provides gene resource for the directional improvementment of sugarcane varieties through genetic engineering.

参考文献/References:

1 赵剑, 杨文杰, 朱蔚华. 细胞色素P450与植物的次生代谢[J]. 生命科学, 1999, 11 (3): 127-131 [Zhao J, Yang WJ, Zhu WH. Cytochrome P450and plant secondary metabolism [J]. Chin Bull Life Sci, 1999, 11 (3): 127-131]2 Shet MS. Relevance of cytochrome P450s in plants: also one of RonEstabrook’s research interests [J]. Drug Metab Rev, 2007, 39 (2-3): 273-2803 Yang CQ, Lu S, Mao YB, Wang LJ, Chen XY. Characterization of twoNADPH: cytochrome P450 reductases from cotton (Gossypium hirsutum)[J]. Phytochemistry, 2010, 71 (1): 27-354 Urban P, Mignotte C, Kazmaier M, Delorme F, Pompon D. Cloning, yeastexpression, and characterization of the coupling of two distantly relatedArabidopsis thaliana NADPH-cytochrome P450 reductases with P450CYP73A5 [J]. J Biol Chem, 1997, 272 (31): 19176-191865 刘惠君, 徐冬梅, 田宝莲, 熊明瑜. 水稻细胞色素P450对酰胺类除草剂催化代谢研究[G]//中国化学会环境化学专业委员会主编. 第四届全国环境化学学术大会论文汇编. 南京: 南京大学环境学院, 2007:263-264 [Liu HJ, Xu DM, Tian BL, Xiong MY. Study the cytochromeP450 metabolic amide herbicide catalytic reaction from rice [G]//Environmental Chemistry Professional Committee of Chinese ChemicalSociety. The fourth Session of National Environmental ChemistryAcademic Conference of Collection of Papers. Nanjing: School ofEnvironment, Nanjing University, 2007: 263-264]6 张斌, 秦岭, 郭凤丹, 杨连群, 边斐, 范仲学, 李娜娜, 宫永超, 蒲艳艳,丁汉凤, 彭振英. 小麦细胞色素P450(TaP450)基因的克隆与序列分析[J]. 麦类作物学报, 2014, 34 (7): 877-884 [Zhang B, Qin L, Guo FD,Yang LQ, Bian F, Fan ZX, Li NN, Gong YC, Pu YY, Ding HF, Peng ZY.Cloning and sequence analysis of a cytochrome P450(TaP450) gene fromwheat (Triticum aestivum L.) [J]. J Tritic Crops, 2014, 34 (7): 877-884]7 张昊, 戴素明, 谢丙炎. 细胞色素P450在茄科作物中的研究进展[J]. 作物研究, 2007, 21 (2): 132-136 [Zhang H, Dai SM, Xie BY. The researchdevelopment of cytochrome P450 in solanaceae crops. Crop Res, 2007,21 (2): 132-136]8 O’Keefe DP, Romesser JA, Leto KJ. Plant and bacterial cytochromesP450 involvement in herbicide metabolism [J]. Recent Adv Phytochem,1987 21: 151-1739 吴翠霞, 吴小虎, 张晓芳, 刘冰, 刘君良. 细胞色素P450酶系对除草剂代谢作用的研究进展[J]. 农药研究与应用, 2008, 12 (6): 8-12 [Wu CX,Wu XH, Zhang XF, Liu B, Liu JL. A review on cytochrome P450s andmetabolisms of herbicides. Agrochem Res Appl, 2008, 12 (6): 8-12]10 凌淑萍, 张会敏, 苏闪闪, 张晓溯, 刘雪艳, 潘桂芳, 袁艺. 洋甘菊细胞色素P450还原酶基因(CPR)的分子克隆及表达分析[J]. 农业生物技术学报, 2014, 22 (5): 580-589 [Ling SP, Zhang HM, Su SS, ZhangXS, Liu XY, Pan GF, Yuan Y. Molecular cloning and characterizationof a cytochrome P450 reductase gene(CPR) full-length in Matricariarecutita [J]. J Agric Biotechnol, 2014, 22 (5): 580-589]11 Jensen K, Moller BL. Plant NADPH-cytochrome P450 oxidoreductases[J]. Phytochemistry, 2010, 71 (2-3): 132-14112 Eberle D, Ullmann P, Werck-Reichhart D, Petersen M. cDNA cloningand functional characterization of CYP98A14 and NADPH: cytochromeP450 reductase from Coleus blumei involved in rosmarinic acidbiosynthesis [J]. Plant Mol Biol, 2009, 69 (3): 239-25313 Ro DK, Ehlting J, Douglas CJ. Cloning, functional expression,and subcellular localization of multiple NADPH-cytochrome P450reductases from hybrid poplar [J]. Plant Physiol, 2002, 130 (4): 1837-185114 Hull AK, Celenza JL. Bacterial expression and purification of theArabidopsis NADPH-cytochrome P450 reductase ATR2 [J]. ProteinExpres Purif, 2000, 18 (3): 310-31515 Hotze M, Schroder G, Schroder J. Cinnamate 4-hydroxylase fromCatharanthus roseus and a strategy for the functional expression ofplant cytochrome P450 proteins as translational fusions with P450reductase in Escherichia coli [J]. FEBS Lett, 1995, 374 (3): 345-35016 阮仁余, 孔建强, 郑晓东, 张书香, 秦咸蕴, 程克棣, 王建明, 王伟. 中国红豆杉细胞色素P450还原酶的基因克隆、表达与活性分析[J]. 遗传, 2010, 32 (11): 1187-1194 [Ruan RY, Kong JQ, Zheng XD, Zhang SX,Qin XY, Cheng KD, Wang JM, Wang W. cDNA cloning, heterologousoverexpression and activity analysis of cytochrome P450 reductase ofTaxus chinensis [J]. Hereditas, 2010, 32 (11): 1187-1194]17 Wang M, Roberts DL, Paschke R, Shea TM, Mastres BS, Kim JJ .Three dimensional structure of NADPH-cytochrome P450 reductase ofprototype for FMN- and FAD-containing enzymes [J]. Proc Natl AcadSci, 1997, 94 (16): 8411-841618 Bolwell GP, Bozak k, Zimmerlin A. Plant cytochrome P450 [J].Phytochemistry, 1994, 37 (6): 1491-150619 Didierjean L, Gondet L, Perkins R, Lau SC, Schaller H, O’Keefe DP,Werck-Reichhart D. Engineering herbicide metabolism in tobaccoand Arabidopsis with CYP76B1, a cytochrome P450 enzyme fromJerusalem artichoke [J]. Plant Physiol, 2002, 130 (1): 179-18920 李明, 曾任森, 骆世明. 细胞色素P450酶系与植物的化学防御[J]. 中国植保导刊, 2006, 26 (11): 13-15 [Li M, Zeng RS, Luo SM. CytochromeP450s and plant chemical defense [J]. China Plant Prot, 2006, 26 (11):13-15]21 Chefson A, Auclair K. Progress towards the easier use of P450 enzymes[J]. Mol Biosys, 2006, 2 (10): 462-46922 Chefson A, Zhao J, Auclair K. Replacement of natural cofactors byselected hydrogen peroxide donors or organic peroxides results inimproved activity for CYP3A4 and CYP2D6 [J]. Chembiochem, 2006, 7(6): 916-91923 Chefson A, Auclair K. CYP3A4 activity in the presence of organiccosolvents, ionic liquids, or water-immiscible organic solvents [J].1140应用与环境生物学报 Chin J Appl Environ Biol http://www.cibj.com/甘蔗细胞色素P450还原酶基因的RT-PCR扩增与表达分析2期Chembiochem, 2007, 8 (10): 1189-119724 苏炜华, 张玉叶, 黄宁, 肖新换, 黄珑, 罗俊, 阙友雄. 甘蔗细胞色素P450还原酶基因的电子克隆与分析[J]. 生物信息学, 2014, 12 (2): 99-105 [Su WH, Zhang YY, Huang N, Xiao XH, Huang L, Luo J, Que YX.Electronic cloning and characterization of sugarcane ScCYP450 geneusing bioinformatics tools [J]. Chin J Bioinformatics, 2014, 12 (2): 99-105]25 Yu Q, Friesen LJS, Zhang XQ, Powles SB. Tolerance to acetolactatesynthase and acetyl-coenzvme A carboxylase inhibiting herbicidesin Vulpia bromoides is conferred by two co-existing resistancemechanisms [J]. Pest Biochem Physiol, 2004, 78 (1): 21-3026 张山, 李平, 刘德立. 细胞色素P450酶系与除草剂代谢[J]. 中国生物工程杂志, 2004, 24 (10): 18-21 [Zhang S, Li P, Liu DL. CytochromeP450s and metabolisms of herbicides [J]. China Biotechnol, 2004, 24 (10):18-21]27 Nafisi M, Sonderby IE, Hansen BG, Geu-Flores F, Auis HHNED,Norholm MHH, Jensen NB, Li J, Halkier BA. Cytochromes P450 in thebiosynthesis of glucosinolates and indole alkaloids [J]. Phytochem Rev,2006. 5 (2): 331-346]28 Chapple C. Molecular genetic analysis of plant cytochrome P450dependent monooxygenases [J]. Physiol Plant Mol Biol, 1998, 49 (1):311-34329 黄珑, 苏炜华, 张玉叶, 黄宁, 凌辉, 肖新换, 阙友雄, 陈如凯. 甘蔗CIPK基因的同源克隆与表达[J]. 作物学报, 2015, 41 (3): 499-506[Huang L, Su WH, Zhang YY, Huang N, Ling H, Xiao XH, Que YX,Chen RK. Cloning and expression analysis of CIPK gene in sugarcane.Acta Agron Sin, 2015, 41 (3): 499-506]30 肖新换, 黄宁, 张玉叶, 杨宗锋, 凌辉, 黄珑, 苏炜华, 阙友雄. 甘蔗光合系统Ⅰ亚基O基因的克隆与表达分析[J]. 应用与环境生物学报,2015, 21 (2): 208-214 [Xiao XH, Huang N, Zhang YY, Yang ZF, LingH, Huang L, Su WH, Que YX. Cloning and expression of photosystemI subunit O gene from sugarcane [J]. Chin J Appl Environ Biol, 2015, 21(2): 208-214]31 肖新换, 黄珑, 黄宁, 张玉叶, 凌辉, 刘峰, 苏炜华, 阙友雄. 甘蔗ScBAK1基因及其可变剪接体的克隆与表达分析[J]. 应用与环境生物学报, 2015, 21 (5): 872-881 [Xiao XH, Huang L, Huang N, ZhangYY, Ling H, Liu F, Su WH, Que YX. Cloning and expression analysis ofScBAK gene and its alternative spliceosome gene in sugarcane [J]. ChinJ Appl Environ Biol, 2015, 21 (5): 872-88132 阙万才, 黄宁, 刘峰, 肖新换, 凌辉, 张玉叶, 苏炜华, 苏亚春, 吴期滨,阙友雄. 甘蔗真核生物翻译起始因子5A基因的克隆与表达分析[J].应用与环境生物学报, 2015, 21 (5): 1120-1127 [Que WC, Huang N,Liu F, Xiao XH, Ling H, Zhang YY, Su WH, Su YC, Wu QB, Que YX.Isolation and expression analysis of a eukaryotic translation initiationfactor 5A gene from sugarcane [J]. Chin J Appl Environ Biol, 2015, 21 (5):1120-112733 Gottschamel J, Waheed MT, Clarke JL, Lossl AG. A novel chloroplasttransformation vector compatible with the Gateway recombinationcloning technology [J]. Transgenic Res, 2013, 22 (6): 1273-127834 朱海龙, 程光远, 彭磊, 柴哲, 郭晋隆, 许莉萍, 徐景升. 甘蔗条纹花叶病毒P3蛋白与甘蔗Rubisco大亚基互作的研究[J]. 西北植物学报,2014, 34 (4): 676-681 [Zhu HL, Cheng GY, Peng L, Cai Z, Guo JL, XuLP, Xu JS. Interaction between sugarcane streak mosaic virus P3 andrubisco large subunit from sugarcane [J]. Acta Bot Bor-Occid Sin, 2014,34 (4): 676-681]35 阙友雄, 许莉萍, 徐景升, 张积森, 张木清, 陈如凯. 甘蔗基因表达定量PCR分析中内参基因的选择[J]. 热带作物学报, 2009, 30 (3): 274-278 [Que YX, Xu LP, Xu JS, Zhang JS, Zhang MQ, Chen RK. Selectionof control genes in Real-time qPCR analysis of gene expression insugarcane [J]. Chin J Trop Crops, 2009, 30 (3): 274-278]36 Livak KJ, Schmittgen TD. Analysis of relative gene expression datausing real-time quantitative PCR and the 2?ΔΔCT method [J]. Methods,2001, 25 (4): 402-40837 王东, 李兵, 林超, 陈玉华, 许雅香, 沈卫德. 家蚕细胞色素P450基因CYP6AE21的克隆、表达分析及亚细胞定位[J]. 昆虫学报, 2011, 54(1): 1-8 [Wang D, Li B, Lin C, Chen YH, Xu YX, Shen WD. Cloning,expression analysis and subcellular location of P450 gene CYP6AE21from Bombyx mori [J]. Acta Entomol Sin, 2011, 54 (1): 1-8]38 李立奇, 万瑛. 蛋白质的亚细胞定位预测研究进展[J]. 免疫学杂志, 2009, 25 (5): 602-604 [Li LQ, Wan Y. Advancement of predictingprotein subcellular localization sites [J]. Immunol J, 2009, 25 (5): 602-604]39 贺丽虹, 赵淑娟, 胡之璧. 植物细胞色素P450基因与功能研究进展[J]. 药物生物技术, 2008, 15 (2): 142-147 [He LH, Zhao SJ, Hu ZB.Gene and function research progress of plant cytochrome P450s [J].Pharm Biotechnol, 2008, 15 (2): 142-147]40 张松焕, 李明泽, 张军, 李春奇. 植物细胞色素P450基因的克隆策略及鉴定方法[J]. 安徽农业科学, 2009, 37 (11): 4911-4914 [Zhang SH,Li MZ, Zhang J, Li CQ. Cloning strategies and identification methods ofplant cytochrome P450 gene [J]. J Anhui Agric Sci, 2009, 37 (11): 4911-4914]41 吴耀荣, 谢旗. ABA与植物胁迫抗性[J]. 植物学通报, 2006, 23 (5):511-518 [Wu YR, Xie Q. ABA and plant stress response [J]. Chin BullBot, 2006, 23 (5): 511-518]42 马崇坚, 柳俊, 谢丛华. 茉莉酸类物质的功能与胁迫防御[J]. 华中农业大学学报, 2001, 20 (16): 603-608 [Ma CJ, Liu J, Xie CH. Jasmonicacid class material defense function and stress [J]. J Huazhong AgricUniv, 2001, 6: 603-608]43 唐红枫. 甲胺磷胁迫下小白菜抗氧化机制研究[D]. 武汉: 华中师范大学, 2006 [Tang HF. Study on the anti-oxygen mechanism of Chinesecabbage under methamidophos [J]. Wuhan: Central China NormalUniversity, 2006]44 孙学娟. CuCl2胁迫对烟草BY-2悬浮细胞死亡的诱导[D]. 泰安: 山东农业大学, 2012 [Sun XJ. Cell death induced by CuCl2 stress in tobaccoBY-2 cells [D]. Tai’an: Shandong Agricultural University, 2012]

相似文献/References:

[1]罗俊,袁照年,张华,等.宿根甘蔗产量性状的稳定性分析[J].应用与环境生物学报,2009,15(04):488.[doi:10.3724/SP.J.1145.2009.00488]
 LUO Jun,YUAN Zhaonian,ZHANG Hua,et al.Stability Analysis on Yield Characters of Sugarcane Ratoon[J].Chinese Journal of Applied & Environmental Biology,2009,15(02):488.[doi:10.3724/SP.J.1145.2009.00488]
[2]叶冰莹,邱思,周平,等.甘蔗蔗糖磷酸合成酶SPSⅡ cDNA片段克隆与表达分析[J].应用与环境生物学报,2011,17(05):673.[doi:10.3724/SP.J.1145.2011.00673]
 YE Bingying,QIU Si,ZHOU Ping,et al.Cloning and Expression Analysis of Sucrose-phosphate Synthase II Gene from Sugarcane[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):673.[doi:10.3724/SP.J.1145.2011.00673]
[3]黄祖新,黄镇,叶冰莹,等.宿根甘蔗根际土壤细菌多样性分析中培养法与非培养法比较研究[J].应用与环境生物学报,2011,17(05):742.[doi:10.3724/SP.J.1145.2011.00742]
 HUANG Zuxin,HUANG Zhen,YE Bingying,et al.Comparison of Culture-dependent and -independent Approaches for Diversity Analysis of Soil Bacteria in the Rhizosphere of Sugarcane[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):742.[doi:10.3724/SP.J.1145.2011.00742]
[4]罗俊,邓祖湖,阙友雄,等.国家甘蔗第七轮区域试验品种的丰产性及稳定性[J].应用与环境生物学报,2012,18(05):734.[doi:10.3724/SP.J.1145.2012.00734]
 LUO Jun,DENG Zuhu,QUE Youxiong,et al.Productivity and Stability of Sugarcane Varieties in the 7th Round National Regional Trial of China[J].Chinese Journal of Applied & Environmental Biology,2012,18(02):734.[doi:10.3724/SP.J.1145.2012.00734]
[5]苏亚春,凌辉,王恒波,等.甘蔗SCoT-PCR反应体系优化与多态性引物筛选及应用[J].应用与环境生物学报,2012,18(05):810.[doi:10.3724/SP.J.1145.2012.00810]
 SU Yachun,LIN Hui,WANG Hengbo,et al.Optimization of SCoT-PCR Reaction System, and Screening and Utilization of Polymorphic Primers in Sugarcane[J].Chinese Journal of Applied & Environmental Biology,2012,18(02):810.[doi:10.3724/SP.J.1145.2012.00810]
[6]肖新换,黄宁,张玉叶,等.甘蔗光合系统Ⅰ亚基O基因的克隆与表达分析[J].应用与环境生物学报,2015,21(02):208.[doi:10.3724/SP.J.1145.2014.09033]
 XIAO Xinhuan,HUANG Ning,ZHANG Yuye,et al.Cloning and expression of photosystem I subunit O gene from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2015,21(02):208.[doi:10.3724/SP.J.1145.2014.09033]
[7]肖新换,黄珑,黄宁,等.甘蔗ScBAK1基因及其可变剪接体的克隆与表达分析[J].应用与环境生物学报,2015,21(05):872.[doi:10.3724/SP.J.1145.2015.03005]
 XIAO Xinhuan,HUANG Long,HUANG Ning,et al.Cloning and expression analysis of ScBAK1 gene and its alternative spliceosome in sugarcane[J].Chinese Journal of Applied & Environmental Biology,2015,21(02):872.[doi:10.3724/SP.J.1145.2015.03005]
[8]阙万才,黄宁,刘峰,等.甘蔗真核生物翻译起始因子5A基因的克隆与表达分析[J].应用与环境生物学报,2015,21(06):1120.[doi:10.3724/SP.J.1145.2015.04008]
 QUE Wancai,HUANG Ning,LIU Feng,et al.Isolation and expression of a eukaryotic translation initiation factor 5A gene from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2015,21(02):1120.[doi:10.3724/SP.J.1145.2015.04008]
[9]苏炜华,黄宁,凌辉,等.甘蔗乙醇脱氢酶基因的克隆与表达分析[J].应用与环境生物学报,2017,23(03):474.[doi:2016.0704]
 SU Weihua,HUANG Ning,LING Hui,et al.Cloning and expression of ScADH from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2017,23(02):474.[doi:2016.0704]
[10]黄宁,李聪娜,汤翰臣,等.甘蔗泛素结合酶基因的克隆与表达[J].应用与环境生物学报,2018,24(04):845.[doi:10.19675/j.cnki.1006-687x.2017.11006]
 HUANG Ning,LI Congna,TANG Hanchen,et al.Cloning and expression analysis of a ubiquitin-conjugating enzyme gene in sugarcane[J].Chinese Journal of Applied & Environmental Biology,2018,24(02):845.[doi:10.19675/j.cnki.1006-687x.2017.11006]

更新日期/Last Update: 2016-04-25