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[1]黄丽杰,李芙蓉,杨志荣,等.拟茎点霉属S4菌株发酵产物对菌核病的防治作用[J].应用与环境生物学报,2015,21(06):1090-1094.[doi:10.3724/SP.J.1145.2015.04055]
 HUANG Lijie,LI Furong,YANG Zhirong,et al.Biocontrol efficacy of Phomopsis sp. strain S4 against Sclerotinia sclerotiorum[J].Chinese Journal of Applied & Environmental Biology,2015,21(06):1090-1094.[doi:10.3724/SP.J.1145.2015.04055]
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拟茎点霉属S4菌株发酵产物对菌核病的防治作用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年06期
页码:
1090-1094
栏目:
研究论文
出版日期:
2015-12-25

文章信息/Info

Title:
Biocontrol efficacy of Phomopsis sp. strain S4 against Sclerotinia sclerotiorum
作者:
黄丽杰 李芙蓉 杨志荣 白林含
四川大学生命科学学院 成都 610064
Author(s):
HUANG Lijie LI Furong YANG Zhirong BAI Linhan
College of Life Sciences, Sichuan University, Chengdu 610064, China
关键词:
内生真菌拟茎点霉属植物抗性核盘菌防治效果
Keywords:
endophytic fungi Phomopsis sp. strain S4 plant defense Sclerotinia sclerotiorum biocontrol effect
分类号:
Q939.9 : S476
DOI:
10.3724/SP.J.1145.2015.04055
文献标志码:
A
摘要:
内生真菌的代谢产物能提高植物对病害的抵抗力,利用其作为生防因子进行植物病害的防治是一种有效的生物防治途径. 以拟南芥为材料,研究内生真菌拟茎点霉属(Phomopsis sp.)S4菌株发酵产物对植物抗病相关基因PDF1.2和PR-1表达水平的影响,并通过盆栽试验评估其对菌核病的防治作用,借助扫描电子显微镜观察S4菌株发酵产物对核盘菌侵染拟南芥的影响. 结果显示,S4菌株发酵产物可以诱导拟南芥抗病相关基因PDF1.2和PR-1持续增量表达. 除了提高植株抗性,S4菌株发酵产物对菌核病有一定的防治效果,能致使核盘菌丝出现皱缩、塌陷等畸形现象,且预防效果明显优于治疗效果. 提前喷洒S4菌株发酵产物能对核盘菌菌丝造成更大程度的损伤,在更大程度上阻碍其侵染进程. 本研究表明,拟茎点霉属S4菌株发酵产物能诱导被病菌侵染的植株产生更强的抗性反应,并且对菌核病有一定的防治效果.
Abstract:
Endophytic fungi are effective means of biological control because their metabolites can improve plant resistance to diseases. This research aimed to investigate the influence of Phomopsis sp. strain S4 on the expression of plant resistance genes PDF1.2 and PR-1 using Arabidopsis thaliana as test material. The effect of Phomopsis sp. strain S4 on the infection of Sclerotinia sclerotiorum was observed through scanning electron microscopy. The results showed that Phomopsis sp. strain S4 could induce the expression of A.thaliana defense genes PDF1.2 and PR-1. In addition to improving plant resistance, Phomopsis sp. strain S4 had a certain control effect against sclerotinia, causing the markedly mycelial alternations of S. sclerotiorum. With the treatment of fermented product of Phomopsis sp. strain S4, the normal hyphae of S. sclerotiorum shriveled and crinkled. The fermented product of Phomopsis sp. strain S4 was greater in preventive effect than in curative effect against S. sclerotiorum. Spraying fermented product of Phomopsis sp. strain S4 before inoculation with pathogen caused more damage to the mycelia of S. sclerotiorum, and blocked infection process at a greater degree. The results indicated that Phomopsis sp. strain S4 can induce the resistance reactions of plants infected by S. sclerotiorum and reduce the severity of sclerotinia diseases.

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备注/Memo:
国际科技合作专项(2015DFR31060)资助 Supported by International Science & Technology Cooperation Program of China (2015DFR31060)
更新日期/Last Update: 2016-01-05