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[1]桑小雪,郭静,白林含.抑制多重耐药致病菌的丹参内生真菌的筛选、鉴定及次生代谢产物分析[J].应用与环境生物学报,2014,20(04):621-628.[doi:10.3724/SP.J.1145.2014.03042]
 SANG Xiaoxue,GUO Jing,BAI Linhan.Isolation, identification and analysis of secondary metabolites of multidrug-resistance inhibiting endophytic fungi of Salvia miltiorrhiza Bge[J].Chinese Journal of Applied & Environmental Biology,2014,20(04):621-628.[doi:10.3724/SP.J.1145.2014.03042]
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抑制多重耐药致病菌的丹参内生真菌的筛选、鉴定及次生代谢产物分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年04期
页码:
621-628
栏目:
研究论文
出版日期:
2014-08-25

文章信息/Info

Title:
Isolation, identification and analysis of secondary metabolites of multidrug-resistance inhibiting endophytic fungi of Salvia miltiorrhiza Bge
作者:
桑小雪 郭静 白林含
四川大学生命科学学院生物资源与生态环境教育部重点实验室 成都 610041
Author(s):
SANG Xiaoxue GUO Jing BAI Linhan
Key Laboratory of Bio-Resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China
关键词:
丹参内生真菌菌株分离鉴定多重耐药致病菌抗菌物质
Keywords:
Salvia miltiorrhiza Bge endophytic fungi isolation and identification multidrug resistant pathogens antibacterial substances
分类号:
Q948.12+2.3 : Q936
DOI:
10.3724/SP.J.1145.2014.03042
文献标志码:
A
摘要:
对四川道地药材丹参内生真菌的抑菌活性及其次生代谢产物进行研究,以期寻找出抑制多重耐药致病菌的新型抗菌物质. 通过分离纯化丹参茎和叶中的内生真菌,得到145株菌株,将菌株发酵液分别进行普通致病菌(肠炎沙门氏菌、鸡白痢沙门氏菌、绿脓杆菌、金黄色葡萄球菌、大肠杆菌O157:H7 和鼠伤寒沙门氏菌)和多重耐药致病菌(沙门氏菌耐药株、金黄色葡萄球菌耐药株和大肠杆菌耐药株)的抑菌试验,筛选得到3株具有显著抑菌效果的菌株. 其中抑菌效果最显著的一株菌株S4,经形态学观察和18S rDNA序列分析确定其为Phomopsis sp. 对S4发酵液的乙酸乙酯萃取相进行大类成分、LC-MS和GC-MS分析,结果显示发酵液中富含酚性物质、生物碱、对羟基苯乙醇等重要生物活性物质. 本研究表明丹参内生真菌抑菌效果显著,富含活性物质,为耐药菌的控制提供了新的资源. 表5 图4 参20
Abstract:
This research aimed to study the antibacterial activity of endophytic fungi isolated from Salvia miltiorrhiza Bge in order to find a new type of antimicrobial substances. One hundred and forty five endophytic fungi were isolated and purified from the stems and leaves of Salvia miltiorrhiza Bge. Three strains with good antibacterial effects were obtained through antibacterial experiments on common poultry pathogens (Salmonella enteritidis, Salmonella pullorum, Pseudomonas aeruginosa, Staphylococcus aureus ATCC 29213, Escherichia coli O157:H7 and Salmonella typhimurium) and multidrug resistant pathogens (the resistant strain of Salmonella, Staphylococcus aureus and Escherichia coli). At last, a strain S4 with the best antibacterial effects was obtained and identified as Phomopsis sp. by morphological observation and 18S rDNA sequence analysis methods. The follow-up experiment included preliminary analysis of the major categories of the chemical composition, LC-MS and GC-MS of the fermentation broth of the S4 strain. The results showed that the fermentation broth contains phenolic compounds, alkaloids and tyrosol with important biological activities. The results provide new resources for control of drug-resistance.

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备注/Memo

备注/Memo:
国家自然科学基金项目(30970043)和科技部国际科技合作专项(2011DFR31220)资助 Supported by the National Natural Science Foundation (30970043) and the International Science & Technology Cooperation Program of China (2011DFR31220)
更新日期/Last Update: 2014-08-26