|本期目录/Table of Contents|

[1]孙建瑞,宋涛,孙显,等.富油小球藻Chlorella protothecoides胞内多糖和油脂提取工艺优化[J].应用与环境生物学报,2014,20(04):615-620.[doi:10.3724/SP.J.1145.2013.12039]
 SUN Jianrui,SONG Tao,SUN Xian,et al.Extraction optimization of intracellular polysaccharide and lipid from oleaginous Chlorella protothecoides[J].Chinese Journal of Applied & Environmental Biology,2014,20(04):615-620.[doi:10.3724/SP.J.1145.2013.12039]
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富油小球藻Chlorella protothecoides胞内多糖和油脂提取工艺优化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年04期
页码:
615-620
栏目:
研究论文
出版日期:
2014-08-25

文章信息/Info

Title:
Extraction optimization of intracellular polysaccharide and lipid from oleaginous Chlorella protothecoides
作者:
孙建瑞 宋涛 孙显 宋济君 王川 乔代蓉
四川大学生命科学学院微生物与代谢工程四川省重点实验室 成都 610064
Author(s):
SUN Jianrui SONG Tao SUN Xian SONG Jijun WANG Chuan QIAO Dairong
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China
关键词:
胞内多糖油脂提取液正交试验设计脂肪酸小球藻
Keywords:
intracellular polysaccharide lipid extraction solution orthogonal array design fatty acid composition Chlorella protothecoides
分类号:
P745 : Q949.21+7.06
DOI:
10.3724/SP.J.1145.2013.12039
文献标志码:
A
摘要:
对小球藻(Chlorella protothecoides)胞内多糖和油脂的提取工艺进行优化,旨在最大程度地提高其高价值代谢产物的综合利用价值. 结果显示,在先提取小球藻胞内多糖、后提取油脂的情况下,不仅胞内多糖可以得到充分的提取,油脂的损失量也很小. 当提取介质的pH为14时,胞内多糖提取量最高,剩余的油脂含量也最高,油脂损失最少. 对胞内多糖提取条件进行正交优化,料液比为主要影响因素,超声时间、提取时间和提取温度为次要因素,最佳工艺条件为:料液比1:25(m/V),超声时间20 min,提取时间180 min,提取温度70 ℃. 在此条件下得到的胞内多糖含量为4.72%,剩余的油脂含量为41.18%. 胞内多糖提取前后小球藻的脂肪酸组成基本上没有变化. 小球藻的脂肪酸组成主要为C16和C18,不饱和脂肪酸含量丰富,占总脂肪酸含量的80%以上.
Abstract:
This research studied intracellular polysaccharide and lipid in Chlorella protothecoides. Extracting intracellular polysaccharide before extraction of lipid could maximumly improve the comprehensive utilization of C. protothecoides, with intracellular polysaccharide fully extracted and the loss of lipid small. The results showed that the best extraction effect of intracellular polysaccharide was obtained with extraction solution pH as 14; in addition, the lipid content of the remaining algae remained at a high level. Extraction of intracellular polysaccharide was optimized by an L9 (34) orthogonal array design (OAD) with four factors at three levels. Solid to?liquid ratio was the main factor influencing extraction of intracellular polysaccharide, while ultrasound time, extraction?temperature and extraction time were the secondary factors. With the optimal extraction condition (solid to?liquid ratio 1:25 (m/V), ultrasonic time 20 min, extraction?temperature 70 oC, and extraction time 180 min), the content of intracellular polysaccharide was 4.72%, with remaining lipid content as 41.18%. The fatty acid composition of C. protothecoides had no significant change after extracting?intracellular polysaccharide. The fatty acids were mainly C16 and C18, rich in unsaturated fatty acids, accounting for?more than 80% of the total fatty acids. This study provides an important research basis and direction for the comprehensive utilization of high-value metabolites from microalgae.

参考文献/References:

1 Valenzuela J, Mazurie A, Carlson RP, Gerlach R, Cooksey KE, Peyton BM, Fields MW. Potential role of multiple carbon fixation pathways during lipid accumulation in Phaeodactylum tricornutum [J]. Biotechnol Biofuels, 2012, 5 (1): 40
2 Menetrez MY. An overview of algae biofuel production and potential environmental impact [J]. Environ Sci Technol, 2012, 46 (13): 7073-7085
3 Liang Y, Mai K, Sun S. Differences in growth, total lipid content and fatty acid composition among 60 clones of Cylindrotheca fusiformis [J]. J Appl Phycol, 2005, 17 (1): 61-65
4 Shi XM, Jiang Y, Chen F. High-yield production of lutein by the green microalga Chlorella protothecoides in heterotrophic fed-batch culture [J]. Biotechnol Prog, 2002, 18 (4): 723-727
5 Mendes-Pinto MM, Raposo MFJ, Bowen J, Young AJ, Morais R. Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects on astaxanthin recovery and implications for bio-availability [J]. J Appl Phycol, 2001, 13 (1): 19-24
6 Hejazi MA, Holwerda E, Wijffels RH. Milking microalga Dunaliella salina for beta-carotene production in two-phase bioreactors [J]. Biotechnol Bioeng, 2004, 85 (5): 475-481
7 Soni B, Trivedi U, Madamwar D. A novel method of single step hydrophobic interaction chromatography for the purification of phycocyanin from Phormidium fragile and its characterization for antioxidant property [J]. Bioresource Technol, 2008, 99 (1): 188-94
8 Hellio C, Marechal JP, Véron B, Bremer G, Clare AS, Le Gal Y. Seasonal variation of antifouling activities of marine algae from the Brittany coast (France) [J]. Mar Biotechnol, 2004, 6 (1): 67-82
9 Qishen P, Guo BJ, Koliman A. Radioprotective effect of extract from Spirulina platensis in mouse bone marrow cells studied by using the micronucleus test [J]. Toxicol Lett, 1989, 48 (2): 165-169
10 Yan C, Yin Y, Zhang D, Yang W, Yu R. Structural characterization and in vitro antitumor activity of a novel polysaccharide from Taxus yunnanensis [J]. Carbohyd Polym, 1993, 96 (2): 389-395
11 Hayashi T, Hayashi K, Maeda M, Kojima I. Calcium spirulan, an inhibitor of enveloped virus replication, from a blue-green alga Spirulina platensis [J]. Nat Prod, 1996, 59 (1): 83-87
12 Fabregas J, Garcia D, Fernandez-Alonso M, Rocha AI, Gómez-Puertas P, Escribano JM, Otero A, Coll JM. In vitro inhibition of the replication of Haemorrhagic septicaemia virus (VHSV) and African swine fever virus (ASFV) by extracts from marine microalgae [J]. Antivial Res, 1999, 44 (l): 67-79
13 陈颖, 李文彬, 孙勇如. 小球藻生物技术研究应用现状及展望[J]. 生物工程进展, 1997, 18 (6): 12-16
14 Wu T, Yan Jun, Liu RH, Marcone MF, Aisa HA, Tsao R. Optimization of microwave-assisted extraction of phenolics from potato and its downstream waste using orthogonal array design [J]. Food Chem, 2012, 133 (4): 1292-1298
15 Christie WW. Lipid Analysis: Isolation, Separation, Identification and Lipidomic Analysis [M]. 4th ed. Bridgewater: Oily Press, 2003
16 Mourente G, Lubián LM, Odriozola JM. Total fatty acid composition as a taxonomic index of some marine microalgae used as food in marine aquaculture [J]. Hydrobiologia, 1990, 203 (3): 147-154
17 De Philippis R, Vincenzini M. Exocellular polysaccharides from cyanobacteria and their possible applications [J]. FEMS Microbiol Rew, 1998, 22 (3): 151-175
18 方积年. 多糖研究的现状[J]. 药学学报, 1986, 21 (12): 944-951
19 曹丽雯, 张福. 正交实验优选盐生藻多糖的提取工艺[J]. 盐业与化工, 2008, 37 (1): 33-35 [Cao LW, Zhang F. Optimum extraction process of polysaccharides from algaes that grow in saltern by orthogonal design [J]. J Salt Chem In, 2008, 37 (1): 33-35]
20 郑维发, 陈才法, 鲍康德, 王义琴, 褚成才. 新月菱形藻胞外多糖的成分及其硫酸酯的制备[J]. 中草药, 2005, 36 (12): 1790-1793 [Zheng WF, Chen CF, Bao KD, Wang YQ, Chu CC. Components of exopolysaccharides of Nitzschia closterium and preparation of their sulfated products [J]. Chin Tradit Herbal Drugs, 2005, 36 (12): 1790-1793]
21 徐锡莲, 童微星, 雷引林, 姚善泾. 盐藻胞外多糖分离纯化方法研究[J]. 食品与生物技术学报, 2007, 26 (4): 28-33 [Xu XL, Tong WX, Lei YL, Yao SJ. Separation and purification of extracellular polysaccharides from Dunaliella salina [J]. J Food Sci Biotechnol, 2007, 26 (4): 28-33]

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备注/Memo

备注/Memo:
“十二五”国家科技支撑计划项目(SB2007FY400-4,2011BAD-14B05,2013BAD10B01)、四川省科技支撑计划项目(2013GZ0058,2012GZ0008)和国家自然科学基金项目(J1103518)资助 Supported by the National Science and Technology Pillar Program of China during the Twelfth Five-year Plan Period (SB2007FY400-4, 2011BAD14B05, 2013BAD10B01), the Science and Technology R & D Program of Sichuan (2013GZ0058, 2012GZ0008), and the National Natural Science Foundation of China (J1103518)
更新日期/Last Update: 2014-08-26