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[1]邓慧华,洪滔,吴承祯,等.马尾松(Pinus massoniana)内生真菌的产油脂效果[J].应用与环境生物学报,2014,20(03):377-381.[doi:10.3724/SP.J.1145.2014.10030]
 DENG Huihua,HONG Tao,WU Chengzhen,et al.Lipid production of endophytic fungi isolated from Pinus massoniana[J].Chinese Journal of Applied & Environmental Biology,2014,20(03):377-381.[doi:10.3724/SP.J.1145.2014.10030]
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马尾松(Pinus massoniana)内生真菌的产油脂效果()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年03期
页码:
377-381
栏目:
研究论文
出版日期:
2014-06-25

文章信息/Info

Title:
Lipid production of endophytic fungi isolated from Pinus massoniana
作者:
邓慧华洪滔吴承祯谢安强林晗洪伟
1福建农林大学林学院 福州 350002 2龙岩市林业科学研究所 龙岩 364000 3福建省高校森林生态系统过程与经营重点实验室 福州 350002 4武夷学院 南平 354300
Author(s):
DENG Huihua HONG Tao WU Chengzhen XIE Anqiang LIN Han HONG Wei
1College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2Longyan City Research Institute of Forestry Sciences, Longyan 364000, China 3Key Laboratory for Forest Ecosystem Process and Management of Fujian Province, Fuzhou 350002, China 4Wuyi University, Nanping 354300, China
关键词:
马尾松内生真菌微生物油脂GC-MS
Keywords:
Pinus massoniana endophytic fungi micrbial lipid GC-MS
分类号:
Q939.9 : Q936
DOI:
10.3724/SP.J.1145.2014.10030
文献标志码:
A
摘要:
从马尾松根、茎、叶分离出21株内生真菌,应用酸热法提取4株优势菌株油脂,测定油脂含量,并采用气相色谱–质谱联用技术(GC-MS)分析油脂组分. 结果表明:4株马尾松内生真菌均含有油脂,油脂含量均达20%以上,最高含量达30.13%. 4株菌都含有脂肪酸,且均占油脂提取物的64%以上,不饱和脂肪酸含量均达46%以上. 油脂组分主要为棕榈酸酯、硬脂酸、油酸、亚油酸、反油酸等,其中油酸含量最大,达42.08%. 因此,马尾松内生真菌可作为一种新的微生物资源用于微生物油脂生产,具有很高的开发利用价值.
Abstract:
This research tried to report for the first time the production of lipid by endophytic fungi isolated from Pinus massoniana, with an objective to promote comprehensive exploitation and utilization of Pinus massoniana. Twenty-one endophytic fungi strains were isolated from the roots, stalks and leaves of Pinus massoniana. Lipids of four dominant strains were extracted by acid-heating method to determine the lipid content and analyze the lipid components by GC-MS. The results showed that all of the four endophytic fungi could produce lipid. The average lipid contents were up to 20%, and the highest content 30.13%. The fatty acid contents of the four endophytic fungi accounted for 64% of the lipids, and the unsaturated fatty acid contents over 46%. The main components of the lipid were palmitate, stearic acid, oleic acid, linoleic acid and elaidic acid etc., with oleic acid of the highest proportion, which was up to 42.08%. Therefore, the endophytic fungi isolated from Pinus massoniana can be used for producing lipid as a new kind of microbial resource with great value of exploitation and utilization.

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备注/Memo

备注/Memo:
国家自然科学基金项目(31070606)资助 Supported by the National Natural Science Foundation of China (31070606)
更新日期/Last Update: 2014-07-01